PMID- 30742765
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 1948-7185 (Electronic)
IS  - 1948-7185 (Linking)
VI  - 10
IP  - 5
DP  - 2019 Mar 7
TI  - Point Mutation of Anabaena Sensory Rhodopsin Enhances Ground-State Hydrogen 
      Out-of-Plane Wag Raman Activity.
PG  - 1012-1017
LID - 10.1021/acs.jpclett.8b03805 [doi]
AB  - The interaction between the retinal protonated Schiff base (RPSB) and surrounding 
      protein residues inside the retinal pocket is believed to play a major role in 
      the ultrafast isomerization of the former. Coherent time-resolved vibrational 
      spectroscopic techniques are applied to reveal the effect of changes in the 
      protein architecture by point mutations (V112N and L83Q) close to the RPSB in 
      Anabaena sensory rhodopsin (ASR). Our study reveals that such point mutations 
      have a minor effect on the low-frequency (<400 cm(-1)) torsional modes but 
      dramatically influence the ground-state vibrational Raman activity of the C(14)-H 
      out-of-plane (HOOP) wag mode (800-820 cm(-1)). In mutated ASR, the increase of 
      HOOP Raman activity in the ground state is experimentally observed for the all- 
      trans RPSB, which has shorter excited-state lifetime than in wild-type ASR. This 
      indicates that predistortion of the RPSB inside the mutated retinal pocket is a 
      major factor in the acceleration of the isomerization rate.
FAU - Roy, Partha Pratim
AU  - Roy PP
AD  - Physikalisch-Chemisches Institut , Ruprecht-Karls Universitat Heidelberg , 
      D-69120 Heidelberg , Germany.
FAU - Abe-Yoshizumi, Rei
AU  - Abe-Yoshizumi R
AD  - Department of Life Science and Applied Chemistry , Nagoya Institute of Technology 
      , Showa-ku, Nagoya 466-8555 , Japan.
FAU - Kandori, Hideki
AU  - Kandori H
AUID- ORCID: 0000-0002-4922-1344
AD  - Department of Life Science and Applied Chemistry , Nagoya Institute of Technology 
      , Showa-ku, Nagoya 466-8555 , Japan.
AD  - OptoBioTechnology Research Center , Nagoya Institute of Technology , Showa-ku, 
      Nagoya 466-8555 , Japan.
FAU - Buckup, Tiago
AU  - Buckup T
AUID- ORCID: 0000-0002-1194-0837
AD  - Physikalisch-Chemisches Institut , Ruprecht-Karls Universitat Heidelberg , 
      D-69120 Heidelberg , Germany.
LA  - eng
PT  - Journal Article
DEP - 20190220
PL  - United States
TA  - J Phys Chem Lett
JT  - The journal of physical chemistry letters
JID - 101526034
RN  - 0 (Sensory Rhodopsins)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Anabaena/*chemistry
MH  - Hydrogen/*chemistry
MH  - *Point Mutation
MH  - Sensory Rhodopsins/chemistry/*genetics
MH  - Spectrum Analysis, Raman
EDAT- 2019/02/12 06:00
MHDA- 2019/03/26 06:00
CRDT- 2019/02/12 06:00
PHST- 2019/02/12 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2019/02/12 06:00 [entrez]
AID - 10.1021/acs.jpclett.8b03805 [doi]
PST - ppublish
SO  - J Phys Chem Lett. 2019 Mar 7;10(5):1012-1017. doi: 10.1021/acs.jpclett.8b03805. 
      Epub 2019 Feb 20.