PMID- 30742923
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20191210
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 129
DP  - 2019 May 15
TI  - Circular RNA circPTK2 regulates oxygen-glucose deprivation-activated 
      microglia-induced hippocampal neuronal apoptosis via 
      miR-29b-SOCS-1-JAK2/STAT3-IL-1beta signaling.
PG  - 488-496
LID - S0141-8130(18)36945-9 [pii]
LID - 10.1016/j.ijbiomac.2019.02.041 [doi]
AB  - Oxygen-glucose deprivation (OGD)-activated microglia contribute to neuronal 
      apoptosis via releasing pro-inflammatory cytokines, and some miRNAs have been 
      reported to be involved in this process. Circular RNAs (circRNAs) have been 
      reported to function as miRNA sponges, but it remains unknown whether and how 
      circRNAs contribute to OGD-activated microglia-induced neuronal apoptosis. Here, 
      we investigated the function and relationship of miR-29b and circPTK2 in 
      OGD-activated microglia-induced neuronal apoptosis. We found upregulation of 
      TNF-alpha and IL-1beta, and downregulation of miR-29b in OGD-activated microglia. 
      miR-29b inhibited OGD-activated microglia-induced neuronal apoptosis. Meanwhile, 
      miR-29b promoted SOCS-1 expression, and suppressed JAK2/STAT3 signaling. In 
      addition, inhibition of JAK2/STAT3 signaling downregulated IL-1beta expression, 
      while upregulation of miR-29b or SOCS-1 also inhibited IL-1beta production. IL-1beta 
      was confirmed to be an apoptosis inducer of hippocampal neurons. Moreover, either 
      SOCS-1 upregulation or blockade of JAK2/STAT3 signaling suppressed OGD-activated 
      microglia-induced neuronal apoptosis. These data suggest that miR-29b inhibits 
      OGD-activated microglia-induced neuronal apoptosis via inducing SOCS-1 
      expression, blocking JNK2/STAT3 signaling, and inhibiting IL-1beta production. 
      circPTK2 was confirmed to inhibit miR-29b expression in OGD model by directly 
      binding to miR-29b. Function assay showed that circPTK2 regulated 
      microglia-induced neuronal apoptosis via sponging miR-29b. Collectively, these 
      findings suggest that circPTK2 regulates OGD-activated microglia-induced neuronal 
      apoptosis via miR-29b-SOCS-1-JAK2/STAT3-IL-1beta signaling.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Wang, Huilin
AU  - Wang H
AD  - Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Li, Zheng
AU  - Li Z
AD  - Clinical Science Institute of Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Gao, Jingyan
AU  - Gao J
AD  - Department of Human Anatomy and Histo-Embryology, Shanghai Medical College of 
      Fudan University, Shanghai, China.
FAU - Liao, Qingwu
AU  - Liao Q
AD  - Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 
      China. Electronic address: liao.qingwu@zs-hospital.sh.cn.
LA  - eng
PT  - Journal Article
DEP - 20190208
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Interleukin-1beta)
RN  - 0 (MIRN29 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Socs1 protein, rat)
RN  - 0 (Suppressor of Cytokine Signaling 1 Protein)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (Janus Kinase 2)
RN  - EC 2.7.10.2 (Ptk2 protein, rat)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Hypoxia/genetics
MH  - Female
MH  - Focal Adhesion Kinase 1/genetics
MH  - Glucose/*metabolism
MH  - Hippocampus/cytology
MH  - Interleukin-1beta/metabolism
MH  - Janus Kinase 2/metabolism
MH  - MicroRNAs/*genetics
MH  - Microglia/*cytology
MH  - Neurons/*cytology
MH  - Oxygen/*metabolism
MH  - Pregnancy
MH  - RNA/*genetics
MH  - RNA, Circular
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - STAT3 Transcription Factor/metabolism
MH  - Signal Transduction/genetics
MH  - Suppressor of Cytokine Signaling 1 Protein/metabolism
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Hippocampal neuron
OT  - Microglia
OT  - Oxygen-glucose deprivation
OT  - circPTK2
OT  - miR-29b
EDAT- 2019/02/12 06:00
MHDA- 2019/07/13 06:00
CRDT- 2019/02/12 06:00
PHST- 2018/12/13 00:00 [received]
PHST- 2019/02/07 00:00 [revised]
PHST- 2019/02/07 00:00 [accepted]
PHST- 2019/02/12 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
PHST- 2019/02/12 06:00 [entrez]
AID - S0141-8130(18)36945-9 [pii]
AID - 10.1016/j.ijbiomac.2019.02.041 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 May 15;129:488-496. doi: 
      10.1016/j.ijbiomac.2019.02.041. Epub 2019 Feb 8.