PMID- 30743980 OWN - NLM STAT- MEDLINE DCOM- 20190308 LR - 20190308 IS - 1879-1026 (Electronic) IS - 0048-9697 (Linking) VI - 660 DP - 2019 Apr 10 TI - Significance of the goby Zosterisessor ophiocephalus as a sentinel species for Venice Lagoon contamination: Combining biomarker responses and bioaccumulation. PG - 959-973 LID - S0048-9697(19)30039-7 [pii] LID - 10.1016/j.scitotenv.2019.01.033 [doi] AB - The Venice Lagoon is an interesting example of an ecosystem suffering for a considerable anthropogenic impact, resulting in high concentrations of persistent organic pollutants (POPs) in lagoon sediments and seafood. In this context, biomonitoring is a crucially important task. The present study aimed at evaluating the validity of a multiple biomarker approach in a benthic fish species. A total of 567 Zosterisessor ophiocephalus (Gobiidae) fish were collected in spring and autumn from three areas of Venice Lagoon (Porto Marghera, Val di Brenta, and Ca Roman) showing high, intermediate and low amounts of POPs, respectively. Aryl hydrocarbon receptor (AHR) and cytochrome P450 1A (CYP1A) mRNA levels, CYP1A protein amount and ethoxyresorufin O-deethylase activity (EROD) were measured in pooled liver and gills (mRNA levels only). Such biological data were then compared with polychlorinated biphenyls (PCBs) residues, measured in grass goby muscle by gas chromatography. Aryl hydrocarbon receptor and CYP1A mRNAs, protein and EROD were upregulated in accordance with PCB amounts measured in Z. ophiocephalus muscles. In fact, the highest AHR and CYP1A induction was observed in fish sampled in close proximity of the industrial area of Porto Marghera. Overall, the present study confirm the grass goby as a reliable sentinel species for Venice Lagoon, and AHR/CYP1A/EROD as a sensitive set of biomarkers of exposure for AHR ligands. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Pauletto, Marianna AU - Pauletto M AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: marianna.pauletto@unipd.it. FAU - Lopparelli, Rosa M AU - Lopparelli RM AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: rosa.lopparelli@unipd.it. FAU - Pegolo, Sara AU - Pegolo S AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: sara.pegolo@unipd.it. FAU - Armani, Mariachiara AU - Armani M AD - Veterinary and Public Health Institute, viale dell'Universita 10, I-35020 Legnaro, Padua, Italy. Electronic address: mariachiara.armani@apss.tn.it. FAU - Zorzan, Eleonora AU - Zorzan E AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: eleonora.zorzan@unipd.it. FAU - Giantin, Mery AU - Giantin M AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: mery.giantin@unipd.it. FAU - Bertotto, Daniela AU - Bertotto D AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: daniela.bertotto@unipd.it. FAU - Gallocchio, Federica AU - Gallocchio F AD - Veterinary and Public Health Institute, viale dell'Universita 10, I-35020 Legnaro, Padua, Italy. Electronic address: fgallocchio@izsvenezie.it. FAU - Zancanella, Vanessa AU - Zancanella V AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. FAU - Capolongo, Francesca AU - Capolongo F AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: francesca.capolongo@unipd.it. FAU - Binato, Giovanni AU - Binato G AD - Veterinary and Public Health Institute, viale dell'Universita 10, I-35020 Legnaro, Padua, Italy. Electronic address: gbinato@izsvenezie.it. FAU - Mutinelli, Franco AU - Mutinelli F AD - Veterinary and Public Health Institute, viale dell'Universita 10, I-35020 Legnaro, Padua, Italy. Electronic address: fmutinelli@izsvenezie.it. FAU - Dacasto, Mauro AU - Dacasto M AD - Department of Comparative Biomedicine and Food Science, University of Padua, viale dell'Universita 16, I-35020 Legnaro, Padua, Italy. Electronic address: mauro.dacasto@unipd.it. LA - eng PT - Journal Article DEP - 20190107 PL - Netherlands TA - Sci Total Environ JT - The Science of the total environment JID - 0330500 RN - 0 (Biomarkers) RN - 0 (Receptors, Aryl Hydrocarbon) RN - 0 (Water Pollutants, Chemical) RN - DFC2HB4I0K (Polychlorinated Biphenyls) RN - EC 1.14.14.1 (Cytochrome P-450 CYP1A1) SB - IM MH - Animals MH - Biomarkers/metabolism MH - Cytochrome P-450 CYP1A1 MH - Environmental Monitoring/*methods MH - Perciformes/*physiology MH - Polychlorinated Biphenyls MH - Receptors, Aryl Hydrocarbon MH - Sentinel Species MH - Water Pollutants, Chemical/*toxicity OTO - NOTNLM OT - Aryl hydrocarbon receptor OT - Biomonitoring OT - Cytochrome P450 1A OT - EROD OT - Pollutants OT - Polychlorinated biphenyls EDAT- 2019/02/13 06:00 MHDA- 2019/03/09 06:00 CRDT- 2019/02/13 06:00 PHST- 2018/09/26 00:00 [received] PHST- 2019/01/04 00:00 [revised] PHST- 2019/01/04 00:00 [accepted] PHST- 2019/02/13 06:00 [entrez] PHST- 2019/02/13 06:00 [pubmed] PHST- 2019/03/09 06:00 [medline] AID - S0048-9697(19)30039-7 [pii] AID - 10.1016/j.scitotenv.2019.01.033 [doi] PST - ppublish SO - Sci Total Environ. 2019 Apr 10;660:959-973. doi: 10.1016/j.scitotenv.2019.01.033. Epub 2019 Jan 7.