PMID- 30744018
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20200225
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 19
IP  - 3
DP  - 2019 Feb 8
TI  - Ultrasensitive Immunosensor Array for TNF-alpha Detection in Artificial Saliva using 
      Polymer-Coated Magnetic Microparticles onto Screen-Printed Gold Electrode.
LID - 10.3390/s19030692 [doi]
LID - 692
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a biomarker of inflammation that occurs in 
      patients suffering from heart failure (HF). Saliva can be sampled in a 
      non-invasive way, and it is currently gaining importance as matrix alternative to 
      blood in diagnostic and therapy monitoring. This work presents the development of 
      an immunosensor array based on eight screen-printed gold electrodes to detect 
      TNF-alpha in saliva samples. Two different functionalization strategies of electrodes 
      were compared. In the first, anti-TNF-alpha antibodies were chemically bonded onto 
      the electrode by functionalization with 4-carboxymethylaniline. The other 
      functionalization procedure involved the binding of antibodies onto 
      polymer-coated magnetic microparticles, which were then deposited onto the 
      electrode by pulsed chronoamperometry. Finally, the chronoamperometry technique 
      was applied to characterize the modified SPEAu. The use of a secondary antibody 
      anti-TNF-alpha (Ab-TNF-alpha-HRP) labelled with horseradish peroxidase (HRP, 2 microg.mL(-1)) 
      was investigated using tetramethylbenzidine (TMB, pH = 3.75) as electrochemical 
      substrate containing 0.2 mM of H(2)O(2). A sandwich-type detection strategy with a 
      secondary antibody anti-TNF-alpha provided chronoamperometric analyses in 10 s for 
      each sample. Linearity, precision, limit of detection, and selectivity of devices 
      were investigated. Interferences were evaluated by analyzing solutions containing 
      other cytokine produced during the acute stage of inflammation. The immunosensor 
      showed good performance within the clinically relevant concentration range, with 
      a precision of 8%, and a limit of detection of 0.3 pg/mL. Therefore, it may 
      represent a promising tool for monitoring HF in a non-invasive way.
FAU - Barhoumi, Lassaad
AU  - Barhoumi L
AD  - NANOMISENE Lab, LR16CRMN01, Centre for Research on Microelectronics and 
      Nanotechnology of Sousse, Technopole of Sousse B.P. 334, Sahloul 4034, Sousse, 
      Tunisia. barhoumilassaad@yahoo.fr.
AD  - University of Sousse, Higher Institute of Applied Sciences and Technology of 
      Sousse, GREENS-ISSAT, Cite Ettafala, Ibn Khaldoun 4003, Sousse, Tunisia. 
      barhoumilassaad@yahoo.fr.
FAU - Bellagambi, Francesca G
AU  - Bellagambi FG
AD  - Department of Chemistry and Industrial Chemistry, University of Pisa, via 
      Giuseppe Moruzzi 13, 56124 Pisa, Italy. francesca.bellagambi@dcci.unipi.it.
FAU - Vivaldi, Federico M
AU  - Vivaldi FM
AD  - Department of Chemistry and Industrial Chemistry, University of Pisa, via 
      Giuseppe Moruzzi 13, 56124 Pisa, Italy. federicomaria.vivaldi@phd.unipi.it.
FAU - Baraket, Abdoullatif
AU  - Baraket A
AD  - Universite de Lyon 1, Institut des Sciences Analytiques, UMR 5280, CNRS, 5 rue de 
      la Doua, 69100 Villeurbanne, France. a.baraket@gmail.com.
FAU - Clement, Yohann
AU  - Clement Y
AD  - Universite de Lyon 1, Institut des Sciences Analytiques, UMR 5280, CNRS, 5 rue de 
      la Doua, 69100 Villeurbanne, France. yohann.clement@isa-lyon.fr.
FAU - Zine, Nadia
AU  - Zine N
AD  - Universite de Lyon 1, Institut des Sciences Analytiques, UMR 5280, CNRS, 5 rue de 
      la Doua, 69100 Villeurbanne, France. nadia.zine@univ-lyon1.fr.
FAU - Ben Ali, Mounir
AU  - Ben Ali M
AD  - NANOMISENE Lab, LR16CRMN01, Centre for Research on Microelectronics and 
      Nanotechnology of Sousse, Technopole of Sousse B.P. 334, Sahloul 4034, Sousse, 
      Tunisia. mounirbenali@yahoo.com.
AD  - University of Sousse, Higher Institute of Applied Sciences and Technology of 
      Sousse, GREENS-ISSAT, Cite Ettafala, Ibn Khaldoun 4003, Sousse, Tunisia. 
      mounirbenali@yahoo.com.
FAU - Elaissari, Abdelhamid
AU  - Elaissari A
AD  - Univ Lyon, University Claude Bernard Lyon-1, CNRS, LAGEP-UMR 5007, F69622 Lyon, 
      France. abdelhamid.elaissari@univ-lyon1.fr.
FAU - Errachid, Abdelhamid
AU  - Errachid A
AD  - Universite de Lyon 1, Institut des Sciences Analytiques, UMR 5280, CNRS, 5 rue de 
      la Doua, 69100 Villeurbanne, France. abdelhamid.errachid@univ-lyon1.fr.
LA  - eng
GR  - 768686/KardiaTool/
GR  - 643694/HEARTEN/
PT  - Journal Article
DEP - 20190208
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Ferrous Compounds)
RN  - 0 (Polymers)
RN  - 0 (Saliva, Artificial)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antibodies, Immobilized/chemistry
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Electrodes
MH  - Ferrous Compounds
MH  - Humans
MH  - Immunoassay/*instrumentation/methods
MH  - Limit of Detection
MH  - Linear Models
MH  - Microspheres
MH  - Polymers/chemistry
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
MH  - Saliva, Artificial/*chemistry
MH  - Tumor Necrosis Factor-alpha/*analysis
PMC - PMC6387098
OTO - NOTNLM
OT  - chronoamperometry
OT  - heart failure
OT  - immunosensor
OT  - magnetic microparticles
OT  - saliva analysis
OT  - tumor necrosis factor-alpha
COIS- The authors declare no conflict of interest.
EDAT- 2019/02/13 06:00
MHDA- 2019/03/05 06:00
PMCR- 2019/02/01
CRDT- 2019/02/13 06:00
PHST- 2018/11/26 00:00 [received]
PHST- 2019/01/29 00:00 [revised]
PHST- 2019/02/01 00:00 [accepted]
PHST- 2019/02/13 06:00 [entrez]
PHST- 2019/02/13 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - s19030692 [pii]
AID - sensors-19-00692 [pii]
AID - 10.3390/s19030692 [doi]
PST - epublish
SO  - Sensors (Basel). 2019 Feb 8;19(3):692. doi: 10.3390/s19030692.