PMID- 30744121
OWN - NLM
STAT- MEDLINE
DCOM- 20190529
LR  - 20200225
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 17
IP  - 2
DP  - 2019 Feb 9
TI  - Immunostimulatory Phosphatidylmonogalactosyldiacylglycerols (PGDG) from the 
      Marine Diatom Thalassiosira weissflogii: Inspiration for a Novel Synthetic 
      Toll-Like Receptor 4 Agonist.
LID - 10.3390/md17020103 [doi]
LID - 103
AB  - An unprecedented phosphatidylmonogalactosyldiacylglycerol pool (PGDG, 1) rich in 
      polyunsaturated fatty acids was isolated from the marine diatoms Thalassiosira 
      weissflogii. Here we report for the first time the NMR characterization of this 
      rare lipid from marine organisms along with a synthetic strategy for the 
      preparation of a PGDG analog (2). PGDG 1 exhibited immunostimulatory activity in 
      human dendritic cells (DCs) and the synthetic PGDG 2 was prepared to explore its 
      mechanism of action. A Toll-like receptor-4 (TLR-4) agonistic activity was 
      evidenced in human and murine DCs underlying the antigen-specific T-cell 
      activation of this class of molecules.
FAU - Manzo, Emiliano
AU  - Manzo E
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. emanzo@icb.cnr.it.
FAU - Gallo, Carmela
AU  - Gallo C
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. carmen.gallo@icb.cnr.it.
FAU - Sartorius, Rossella
AU  - Sartorius R
AD  - CNR-Institute of Protein Biochemistry, via Pietro Castellino, 111 - 80131 Napoli, 
      Italy. r.sartorius@ibp.cnr.it.
FAU - Nuzzo, Genoveffa
AU  - Nuzzo G
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. nuzzo.genoveffa@icb.cnr.it.
FAU - Sardo, Angela
AU  - Sardo A
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. angela.sardo@icb.cnr.it.
FAU - De Berardinis, Piergiuseppe
AU  - De Berardinis P
AD  - CNR-Institute of Protein Biochemistry, via Pietro Castellino, 111 - 80131 Napoli, 
      Italy. p.deberardinis@ibp.cnr.it.
FAU - Fontana, Angelo
AU  - Fontana A
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. afontana@icb.cnr.it.
FAU - Cutignano, Adele
AU  - Cutignano A
AD  - CNR-Institute of Biomolecular Chemistry, via Campi Flegrei, 34 - 80078 Pozzuoli 
      (Napoli), Italy. acutignano@icb.cnr.it.
LA  - eng
GR  - PON01_00117/Italian MIUR/International
PT  - Journal Article
DEP - 20190209
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Glycolipids)
RN  - 0 (Phosphatidylglycerols)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (phosphatidylmonoglycosyl diacylglycerol)
SB  - IM
MH  - Adjuvants, Immunologic/chemical synthesis/*chemistry/isolation & 
      purification/*pharmacology
MH  - Animals
MH  - Carbon-13 Magnetic Resonance Spectroscopy
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/immunology
MH  - Diatoms/*chemistry
MH  - Female
MH  - Glycolipids/chemical synthesis/*chemistry/isolation & purification/*pharmacology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Structure
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Phosphatidylglycerols/chemical synthesis/*chemistry/isolation & 
      purification/*pharmacology
MH  - Toll-Like Receptor 4/*agonists/immunology
PMC - PMC6409857
OTO - NOTNLM
OT  - Thalassiosira weissflogii
OT  - Toll-like receptor 4
OT  - dendritic cells
OT  - immunostimulatory lipid
OT  - marine diatom
OT  - phosphogalactolipids
OT  - phosphoglycolipid synthesis
COIS- The authors declare no conflict of interest.
EDAT- 2019/02/13 06:00
MHDA- 2019/05/30 06:00
PMCR- 2019/02/01
CRDT- 2019/02/13 06:00
PHST- 2019/01/16 00:00 [received]
PHST- 2019/02/04 00:00 [revised]
PHST- 2019/02/05 00:00 [accepted]
PHST- 2019/02/13 06:00 [entrez]
PHST- 2019/02/13 06:00 [pubmed]
PHST- 2019/05/30 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - md17020103 [pii]
AID - marinedrugs-17-00103 [pii]
AID - 10.3390/md17020103 [doi]
PST - epublish
SO  - Mar Drugs. 2019 Feb 9;17(2):103. doi: 10.3390/md17020103.