PMID- 30744186 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 8 IP - 2 DP - 2019 Feb 10 TI - Frequent Alteration of Annexin A9 and A10 in HPV-Negative Head and Neck Squamous Cell Carcinomas: Correlation with the Histopathological Differentiation Grade. LID - 10.3390/jcm8020229 [doi] LID - 229 AB - The annexin protein superfamily has been implicated in multiple physiological and pathological processes, including carcinogenesis. Altered expression of various annexins has frequently been observed and linked to the development and progression of various human malignancies. However, information is lacking on the expression and clinical significance of annexin A9 (ANXA9) and A10 (ANXA10) in head and neck squamous cell carcinomas (HNSCC). ANXA9 and ANXA10 expression was evaluated in a large cohort of 372 surgically treated HPV-negative HNSCC patients and correlated with the clinicopathologic parameters and disease outcomes. Down-regulation of ANXA9 expression was found in 42% of HNSCC tissue samples, compared to normal epithelia. ANXA9 expression in tumors was significantly associated with oropharyngeal location and histological differentiation grade (P < 0.001). In marked contrast, ANXA10 expression was absent in normal epithelium, but variably detected in the cytoplasm of cancer cells. Positive ANXA10 expression was found in 64% of tumors, and was significantly associated with differentiation grade (P < 0.001), being also more frequent in oropharyngeal tumors (P = 0.019). These results reveal that the expression of both ANXA9 and ANXA10 is frequently altered in HNSCC and associated to the tumor differentiation grade, suggesting that they could be implicated in the pathogenesis of these cancers. FAU - Salom, Cecilia AU - Salom C AD - Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. mariaceciliasalom@gmail.com. FAU - Alvarez-Teijeiro, Saul AU - Alvarez-Teijeiro S AD - Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. saul.teijeiro@gmail.com. AD - CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain. saul.teijeiro@gmail.com. FAU - Fernandez, M Pilar AU - Fernandez MP AD - Department of Biochemistry and Molecular Biology and Institute of Biotechnology of Asturias, University of Oviedo, Julian Claveria, 33006, Oviedo, Spain. pfernandez@uniovi.es. FAU - Morgan, Reginald O AU - Morgan RO AD - Department of Biochemistry and Molecular Biology and Institute of Biotechnology of Asturias, University of Oviedo, Julian Claveria, 33006, Oviedo, Spain. morganreginald@uniovi.es. FAU - Allonca, Eva AU - Allonca E AD - Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. ynkc1@hotmail.com. AD - CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain. ynkc1@hotmail.com. FAU - Vallina, Aitana AU - Vallina A AD - Department of Pathology, Hospital Universitario Central de Asturias and Instituto Universitario de Oncologia del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. alaicla@hotmail.es. FAU - Lorz, Corina AU - Lorz C AD - CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain. clorz@ciemat.es. AD - Molecular Oncology Unit, CIEMAT (ed 70A), Av. Complutense 40, 28040 Madrid, Spain. clorz@ciemat.es. FAU - de Villalain, Lucas AU - de Villalain L AD - Department of Oral Surgery, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. lvillalain@hotmail.com. FAU - Fernandez-Garcia, M Soledad AU - Fernandez-Garcia MS AD - Department of Pathology, Hospital Universitario Central de Asturias and Instituto Universitario de Oncologia del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. solefghdr@hotmail.com. FAU - Rodrigo, Juan P AU - Rodrigo JP AD - Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. jprodrigo@uniovi.es. AD - CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain. jprodrigo@uniovi.es. FAU - Garcia-Pedrero, Juana M AU - Garcia-Pedrero JM AD - Department of Otolaryngology, Hospital Universitario Central de Asturias and Instituto de Investigacion Sanitaria del Principado de Asturias, University of Oviedo, Avda. Roma, 33011, Oviedo, Spain. juanagp.finba@gmail.com. AD - CIBERONC, Av. Monforte de Lemos, 3-5. 28029, Madrid, Spain. juanagp.finba@gmail.com. LA - eng GR - PI13/00259 and PI16/00280/Instituto de Salud Carlos III/ GR - CIBERONC (CB16/12/00390)/Instituto de Salud Carlos III/ PT - Journal Article DEP - 20190210 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC6406441 OTO - NOTNLM OT - annexin A10 OT - annexin A9 OT - differentiation grade OT - head and neck squamous cell carcinoma OT - immunohistochemistry COIS- The authors declare no conflicts of interest. EDAT- 2019/02/13 06:00 MHDA- 2019/02/13 06:01 PMCR- 2019/02/10 CRDT- 2019/02/13 06:00 PHST- 2018/12/03 00:00 [received] PHST- 2019/01/30 00:00 [revised] PHST- 2019/02/04 00:00 [accepted] PHST- 2019/02/13 06:00 [entrez] PHST- 2019/02/13 06:00 [pubmed] PHST- 2019/02/13 06:01 [medline] PHST- 2019/02/10 00:00 [pmc-release] AID - jcm8020229 [pii] AID - jcm-08-00229 [pii] AID - 10.3390/jcm8020229 [doi] PST - epublish SO - J Clin Med. 2019 Feb 10;8(2):229. doi: 10.3390/jcm8020229.