PMID- 30744815
OWN - NLM
STAT- MEDLINE
DCOM- 20190301
LR  - 20190301
IS  - 1618-0623 (Electronic)
IS  - 0944-5013 (Linking)
VI  - 220
DP  - 2019 Mar
TI  - Screening, plant growth promotion and root colonization pattern of two 
      rhizobacteria (Pseudomonas fluorescens Ps006 and Bacillus amyloliquefaciens 
      Bs006) on banana cv. Williams (Musa acuminata Colla).
PG  - 12-20
LID - S0944-5013(18)30729-8 [pii]
LID - 10.1016/j.micres.2018.11.006 [doi]
AB  - Banana is the second largest export crop in Colombia. To meet the demand of 
      international markets, high amounts of chemical fertilizers are required, which 
      represent high costs and can be hazardous to the environment. Plant growth 
      promoting rhizobacteria (PGPR) can, at least partially, replace chemical 
      fertilizers. In this paper, we evaluated the effect of nine PGPR of the genera 
      Bacillus and Pseudomonas on banana growth. Banana seedlings were produced through 
      tissue culture and acclimatized in the greenhouse core. Plants were inoculated 
      with the rhizobacteria and growth parameters (plant height, leaf number, leaf 
      area, pseudostem thickness, root and shoot fresh weight, root and shoot dry 
      weight) were assessed after 55 days. The two best performing PGPR, Bs006 and 
      Ps006 previously identified as Bacillus amyloliquefaciens and Pseudomonas 
      fluorescens, respectively, promoted banana growth similarly or even slightly 
      superior to 100% chemical fertilization, and were selected for further 
      characterization of root colonization by both eletron microscopy and confocal 
      microscopy of fluorescence in situ hybridization (FISH)-stained root tissues. 
      Both P. fluorescens Ps006 and B. amyloquifaciens Bs006 showed ability to colonize 
      banana roots, but Bs006 appeared faster than Ps006 in the colonization dynamics. 
      This work demonstrated that inoculation of rhizobacteria Bacillus 
      amyloliquefaciens Bs006 and Pseudomonas fluorescens Ps006 could partially replace 
      the chemical fertilization of tissue cultured banana plants, and therefore could 
      be used for the formulation of a new biofertilizer.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Gamez, R
AU  - Gamez R
AD  - Colombian Corporation of Agricultural Research, AGROSAVIA, C.I. Turipana, 
      Monteria, Colombia; University of La Sabana, Chia, Colombia.
FAU - Cardinale, M
AU  - Cardinale M
AD  - Institute of Applied Microbiology, Justus-Liebig-University, Giessen, Germany. 
      Electronic address: Massimiliano.Cardinale@umwelt.uni-giessen.de.
FAU - Montes, M
AU  - Montes M
AD  - Colombian Corporation of Agricultural Research, AGROSAVIA, C.I. Turipana, 
      Monteria, Colombia.
FAU - Ramirez, S
AU  - Ramirez S
AD  - Colombian Corporation of Agricultural Research, AGROSAVIA, C.I. Tibaitata, 
      Mosquera, Colombia.
FAU - Schnell, S
AU  - Schnell S
AD  - Institute of Applied Microbiology, Justus-Liebig-University, Giessen, Germany.
FAU - Rodriguez, F
AU  - Rodriguez F
AD  - Colombian Corporation of Agricultural Research, AGROSAVIA, C.I. Tibaitata, 
      Mosquera, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20181123
PL  - Germany
TA  - Microbiol Res
JT  - Microbiological research
JID - 9437794
RN  - 0 (Fertilizers)
RN  - 0 (Soil)
SB  - IM
MH  - Bacillus amyloliquefaciens/genetics/isolation & purification/*physiology
MH  - Colombia
MH  - Fertilizers
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Electron, Scanning
MH  - Musa/*growth & development/*microbiology
MH  - *Plant Development
MH  - Plant Leaves/growth & development
MH  - Plant Roots/*microbiology
MH  - Pseudomonas fluorescens/genetics/isolation & purification/*physiology
MH  - Seedlings
MH  - Soil/chemistry
MH  - Soil Microbiology
OTO - NOTNLM
OT  - Bacillus
OT  - Banana cv. Williams
OT  - Fluorescence in situ hybridization-confocal laser scanning microscopy (FISH-CLSM)
OT  - Plant growth promoting rhizobacteria (PGPR)
OT  - Pseudomonas
OT  - Scanning electron microscopy (SEM)
EDAT- 2019/02/13 06:00
MHDA- 2019/03/02 06:00
CRDT- 2019/02/13 06:00
PHST- 2018/06/20 00:00 [received]
PHST- 2018/10/10 00:00 [revised]
PHST- 2018/11/22 00:00 [accepted]
PHST- 2019/02/13 06:00 [entrez]
PHST- 2019/02/13 06:00 [pubmed]
PHST- 2019/03/02 06:00 [medline]
AID - S0944-5013(18)30729-8 [pii]
AID - 10.1016/j.micres.2018.11.006 [doi]
PST - ppublish
SO  - Microbiol Res. 2019 Mar;220:12-20. doi: 10.1016/j.micres.2018.11.006. Epub 2018 
      Nov 23.