PMID- 30747410
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20190405
LR  - 20200212
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Linking)
VI  - 30
IP  - 4
DP  - 2019 Apr
TI  - Quantitation of Glutathione by Quinoline-5, 8-Dione-Based Tag Strategy Using 
      MALDI Mass Spectrometry.
PG  - 625-633
LID - 10.1007/s13361-019-02135-4 [doi]
AB  - In the present work, we developed an UV-absorptive and highly reactive tag 
      aromatic molecule, quinoline-5,8-dione (QLD), for robust quantitative analysis of 
      GSH by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The 
      QLD could react with GSH with high efficiency at room temperature, and the 
      resultant QLD-GSH conjugate could be readily detected by MALDI MS without 
      interferences. By using the QLD tag, the detection limit of GSH was lowered to 
      10 fmol muL(-1), which was four orders of magnitude higher than that detected 
      without using the QLD tag. Furthermore, accurate quantitative measurements of GSH 
      in solution were successfully demonstrated by using glutamic 
      acid-cysteine-alanine (ECA) as an internal standard. By properly adjusting the 
      ECA concentrations, the intensity ratio value of QLD-tagged GSH (QLD-GSH) to 
      QLD-tagged ECA (QLD-ECA) displayed a good linearity with GSH concentrations in a 
      broad range from 4 to 4000 muM. Finally, the GSH level in HeLa cell lysates was 
      also successfully detected, and the results are consistent with that obtained by 
      a colorimetric assay. In summary, the proposed QLD-based tag method should be a 
      rapid, cost-/time-effective, and sensitive new method for quantitative 
      determination of GSH by MALDI MS. Graphical Abstract.
FAU - Guo, Liming
AU  - Guo L
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, China.
FAU - Xiao, Chunsheng
AU  - Xiao C
AD  - Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, 
      Chinese Academy of Sciences, Changchun, 130022, China. xiaocs@ciac.ac.cn.
FAU - Wang, Sheng
AU  - Wang S
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, China.
FAU - Gao, Tianyang
AU  - Gao T
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, China.
FAU - Ling, Ling
AU  - Ling L
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, China.
FAU - Guo, Xinhua
AU  - Guo X
AUID- ORCID: 0000-0002-3503-0440
AD  - State Key Laboratory of Supramolecular Structure and Materials, College of 
      Chemistry, Jilin University, Changchun, 130012, China. guoxh@jlu.edu.cn.
AD  - Key Laboratory for Molecular Enzymology and Engineering of the Ministry of 
      Education, College of Life Science, Jilin University, Changchun, 130012, China. 
      guoxh@jlu.edu.cn.
LA  - eng
GR  - 21874054/National Natural Science Foundation of China/
GR  - 21675060/National Natural Science Foundation of China/
GR  - 21175056/National Natural Science Foundation of China/
GR  - 2017266/Youth Innovation Promotion Association of the Chinese Academy of 
      Sciences/
PT  - Journal Article
DEP - 20190211
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
OTO - NOTNLM
OT  - Cells
OT  - Glutathione
OT  - Matrix-assisted laser desorption/ionization mass spectrometry
OT  - Quantification
EDAT- 2019/02/13 06:00
MHDA- 2019/02/13 06:01
CRDT- 2019/02/13 06:00
PHST- 2018/10/19 00:00 [received]
PHST- 2019/01/08 00:00 [accepted]
PHST- 2018/12/28 00:00 [revised]
PHST- 2019/02/13 06:00 [pubmed]
PHST- 2019/02/13 06:01 [medline]
PHST- 2019/02/13 06:00 [entrez]
AID - 10.1007/s13361-019-02135-4 [pii]
AID - 10.1007/s13361-019-02135-4 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2019 Apr;30(4):625-633. doi: 10.1007/s13361-019-02135-4. 
      Epub 2019 Feb 11.