PMID- 3074744
OWN - NLM
STAT- MEDLINE
DCOM- 19890710
LR  - 20071115
IS  - 0004-069X (Print)
IS  - 0004-069X (Linking)
VI  - 36
IP  - 4
DP  - 1988
TI  - Antigenic phenotype of chronic granulocytic leukaemia progenitor cells in chronic 
      phase and in blastic transformation characterized by means of monoclonal 
      antibodies.
PG  - 471-85
AB  - We studied the surface antigens of "early" and "late" granulocyte-macrophage 
      progenitor cells (CFU-GM) from bone marrow and peripheral blood of 18 patients 
      with chronic granulocytic leukaemia in chronic phase (CGL-CP) using 14 selected 
      murine monoclonal antibodies (McAbs) in complement dependent cytotoxicity assay 
      followed by culture in methyl cellulose. The same panel of McAbs was used to 
      determine the antigens on leukaemic blast cells from peripheral blood of 15 
      patients with CGL in blastic transformation (BT) by complement mediated lysis and 
      in vitro culture technique for clonogenic blasts (CFU-L) and immunofluorescence 
      assay for total blast population. McAb for HLA-DR antigens (L243) and McAbs MY9, 
      S3-13, S17-25 and 53/6 reacted with CFV-GM and CFU-L. In contrast, McAbs PM81 and 
      AML-2-23 recognizing antigens on more mature myeloid cells did not react with 
      these progenitor cells. McAb S4-7 reacted with the majority of CFU-L and a small 
      proportion of "early" and "late" CFU-GM. This McAb may be useful for the 
      prediction of blastic transformation in CGL patients. Generally, the reactivity 
      of most McAbs was more heterogeneous with CFU-L than with CFU-GM in individual 
      patients. The majority of McAbs included in our study reacted with a higher 
      percentage of CFU-L and CFU-GM than predominant blast cell population in 
      individual patients perhaps because the detected antigens are expressed more 
      strongly on dividing progenitors than on relatively nonproliferative progeny. 
      Thus we interpret the results of these studies showing that the antigenic 
      phenotypes of the blast colony progenitor cells in CGL-BT are very similar to but 
      not identical with those of CFU-GM from CGL-CP patients.
FAU - Robak, T
AU  - Robak T
AD  - Department of Pharmacology, Medical Academy, Lodz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Arch Immunol Ther Exp (Warsz)
JT  - Archivum immunologiae et therapiae experimentalis
JID - 0114365
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Antibodies, Monoclonal
MH  - Antigens, Neoplasm/*analysis
MH  - Antigens, Surface/immunology
MH  - Blast Crisis/*immunology
MH  - Bone Marrow Cells
MH  - Cells, Cultured
MH  - Cytotoxicity Tests, Immunologic
MH  - Fluorescent Antibody Technique
MH  - Hematopoietic Stem Cells/*immunology
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*blood/pathology
MH  - Middle Aged
MH  - Phenotype
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Arch Immunol Ther Exp (Warsz). 1988;36(4):471-85.