PMID- 30756390
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 9
DP  - 2019 Sep
TI  - Circular RNA expression profiles during the differentiation of human umbilical 
      cord-derived mesenchymal stem cells into cardiomyocyte-like cells.
PG  - 16412-16423
LID - 10.1002/jcp.28310 [doi]
AB  - So far, there were no reports on circular RNA (circRNA) expression profiles in 
      the differentiation of human umbilical cord-derived mesenchymal stem cells 
      (hUCMSCs) into cardiomyocyte-like cells induced by 5-aza. In this study, hUCMSCs 
      were isolated from umbilical cords and induced with 5-aza for 14 days. 
      Immunofluorescence staining, real-time reverse transcription polymerase chain 
      reaction (RT-PCR), and western blot of cardiac troponin I and alpha-sarcomeric actin 
      on hUCMSCs between Days 14 and 0 were performed. The expression profile of 
      circRNAs was analyzed by microarray and validated with RT-PCR. Gene Ontology (GO) 
      and Kyoto Encyclopedia of Genes and Genomes analyses were performed to identify 
      the functions of differentially expressed genes and related pathways. The 
      connections between circRNAs and microRNAs were explored by using Cytoscape. The 
      results showed that a total of 226 circRNAs were calculated as differentially 
      expressed during the differentiation. Among them, 127 were upregulated and 99 
      were downregulated. We selected circRNAs that were upregulated by more than 
      five-fold and downregulated by more than three-fold. Ultimately, 74 
      differentially expressed circRNAs that were highly conserved on Day 14 after 
      induction compared to Day 0 were identified. Among them, 41 were upregulated and 
      33 were downregulated. Four upregulated circRNAs (circRNA_01536, circRNA_04411, 
      circRNA_09169, and circRNA_09905) and four downregulated circRNAs (circRNA_00699, 
      circRNA_01183, circRNA_01978, and circRNA_16804) were randomly confirmed by 
      RT-PCR. GO analysis suggested a number of cell proliferation and differentiation 
      related physiological processes and pathways, such as the Wnt signaling pathway 
      and others. Network analysis uncovered three potential key circRNAs, that is, 
      circRNA_05432, circRNA_08441, and circRNA_01536.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Ruan, Zhong-Bao
AU  - Ruan ZB
AUID- ORCID: 0000-0003-0607-0010
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, P.R. China.
FAU - Chen, Ge-Cai
AU  - Chen GC
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, P.R. China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, P.R. China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Jiangsu Taizhou People's Hospital, Taizhou, P.R. China.
LA  - eng
GR  - BK20161370/Natural Science Foundation of Jiangsu Province/
PT  - Journal Article
DEP - 20190212
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
SB  - IM
OTO - NOTNLM
OT  - cardiomyocyte-like cells
OT  - circular RNAs
OT  - differentiation
OT  - expression profile
OT  - human umbilical cord-derived mesenchymal stem cells
EDAT- 2019/02/14 06:00
MHDA- 2019/02/14 06:01
CRDT- 2019/02/14 06:00
PHST- 2019/01/24 00:00 [revised]
PHST- 2018/12/03 00:00 [received]
PHST- 2019/01/28 00:00 [accepted]
PHST- 2019/02/14 06:00 [pubmed]
PHST- 2019/02/14 06:01 [medline]
PHST- 2019/02/14 06:00 [entrez]
AID - 10.1002/jcp.28310 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Sep;234(9):16412-16423. doi: 10.1002/jcp.28310. Epub 2019 
      Feb 12.