PMID- 30758701 OWN - NLM STAT- MEDLINE DCOM- 20190701 LR - 20200225 IS - 1573-5168 (Electronic) IS - 0920-1742 (Linking) VI - 45 IP - 2 DP - 2019 Apr TI - Identification of proteins differentially expressed in the gills of grass carp (Ctenopharyngodon idella) after hypoxic stress by two-dimensional gel electrophoresis analysis. PG - 743-752 LID - 10.1007/s10695-018-0599-5 [doi] AB - Two-dimensional gel electrophoresis (2-DE) was combined with liquid chromatography-mass spectrometry (LC-MS/MS) to identify the differential proteomics of grass carp gills after hypoxic stress to better understand the roles of proteins in the hypoxic response and to explore the possible molecular mechanisms. Protein spots were obtained from a hypoxia-stressed group (372 +/- 11 individuals) and a control group (406 +/- 14 individuals) using the lmage Master 2D Platinum 7.0 analysis software. Fifteen protein spots were expressed differentially in the hypoxia-stressed group and varied significantly after exposure to the hypoxic conditions. In addition, these differential proteins were identified by mass spectrometry and then searched in a database. We found the expression and upregulation of the toll-like receptor 4, ephx1 protein, isocitrate dehydrogenase, L-lactate dehydrogenase, GTP-binding nuclear protein Ran, and glyceraldehyde-3-phosphate dehydrogenase; however, the expression of the keratin type II cytoskeletal 8, type I cytokeratin, ARP3 actin-related protein 3 homolog, thyroid hormone receptor alpha-A, ATP synthase subunit beta, citrate synthase, tropomyosin 2, and tropomyosin 3 were downregulated. Six proteins were found in the hypoxia-inducible factor-1 (HIF-1) signaling pathway. We concluded that the grass carp gill is involved in response processes, including energy generation, metabolic processes, cellular structure, antioxidation, immunity, and signal transduction, to hypoxic stress. To our knowledge, this is the first study to conduct a proteomics analysis of expressed proteins in the gills of grass carp, and this study will help increase the understanding of the molecular mechanisms involved in hypoxic stress responses in fish at the protein level. FAU - Xu, Zhan-Ning AU - Xu ZN AD - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. AD - Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. FAU - Zheng, Guo-Dong AU - Zheng GD AD - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. AD - Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. FAU - Wu, Cheng-Bin AU - Wu CB AD - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. AD - Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. FAU - Jiang, Xia-Yun AU - Jiang XY AD - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. jiangxy@shou.edu.cn. AD - Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. jiangxy@shou.edu.cn. AD - Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China. jiangxy@shou.edu.cn. FAU - Zou, Shu-Ming AU - Zou SM AUID- ORCID: 0000-0003-3522-8390 AD - Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. smzou@shou.edu.cn. AD - Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. smzou@shou.edu.cn. AD - Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China. smzou@shou.edu.cn. LA - eng GR - 31572220/National Natural Science Foundation of China/ GR - 2012BAD26B02/Key Technologies Research and Development Program of China/ PT - Journal Article DEP - 20190213 PL - Netherlands TA - Fish Physiol Biochem JT - Fish physiology and biochemistry JID - 100955049 RN - 0 (Fish Proteins) RN - 0 (Hypoxia-Inducible Factor 1) RN - 059QF0KO0R (Water) RN - S88TT14065 (Oxygen) SB - IM MH - Adaptation, Physiological MH - Animals MH - Carps/*metabolism MH - Fish Proteins/genetics/*metabolism MH - Gene Expression Regulation/*drug effects MH - Gills/*anatomy & histology/*metabolism MH - Hypoxia-Inducible Factor 1/genetics/metabolism MH - Oxygen/*administration & dosage/chemistry/pharmacology MH - Signal Transduction/drug effects MH - Water/chemistry OTO - NOTNLM OT - Gill OT - Grass carp OT - Hypoxic stress OT - Mass spectrum OT - Two-dimensional electrophoresis EDAT- 2019/02/14 06:00 MHDA- 2019/07/02 06:00 CRDT- 2019/02/14 06:00 PHST- 2018/07/10 00:00 [received] PHST- 2018/11/29 00:00 [accepted] PHST- 2019/02/14 06:00 [pubmed] PHST- 2019/07/02 06:00 [medline] PHST- 2019/02/14 06:00 [entrez] AID - 10.1007/s10695-018-0599-5 [pii] AID - 10.1007/s10695-018-0599-5 [doi] PST - ppublish SO - Fish Physiol Biochem. 2019 Apr;45(2):743-752. doi: 10.1007/s10695-018-0599-5. Epub 2019 Feb 13.