PMID- 30760482
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20191230
IS  - 1477-9129 (Electronic)
IS  - 0950-1991 (Linking)
VI  - 146
IP  - 5
DP  - 2019 Mar 1
TI  - Alternative use of miRNA-biogenesis co-factors in plants at low temperatures.
LID - dev172932 [pii]
LID - 10.1242/dev.172932 [doi]
AB  - Plants use molecular mechanisms to sense temperatures, trigger quick adaptive 
      responses and thereby cope with environmental changes. MicroRNAs (miRNAs) are key 
      regulators of plant development under such conditions. The catalytic action of 
      DICER LIKE 1 (DCL1), in conjunction with HYPONASTIC LEAVES 1 (HYL1) and SERRATE 
      (SE), produces miRNAs from double-stranded RNAs. As plants lack a stable internal 
      temperature to which enzymatic reactions could be optimized during evolution, 
      reactions such as miRNA processing have to be adjusted to fluctuating 
      environmental temperatures. Here, we report that with decreasing ambient 
      temperature, the plant miRNA biogenesis machinery becomes more robust, producing 
      miRNAs even in the absence of the key DCL1 co-factors HYL1 and SE. This reduces 
      the morphological and reproductive defects of se and hyl1 mutants, restoring seed 
      production. Using small RNA-sequencing and bioinformatics analyses, we have 
      identified specific miRNAs that become HYL1/SE independent for their production 
      in response to temperature decrease. We found that the secondary structure of 
      primary miRNAs is key for this temperature recovery. This finding may have 
      evolutionary implications as a potential adaptation-driving mechanism to a 
      changing climate.
CI  - (c) 2019. Published by The Company of Biologists Ltd.
FAU - Re, Delfina A
AU  - Re DA
AD  - Instituto de Agrobiotecnologia del Litoral (CONICET-UNL), Facultad de Bioquimica 
      y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe 3000, 
      Argentina.
FAU - Lang, Patricia L M
AU  - Lang PLM
AD  - Instituto de Agrobiotecnologia del Litoral (CONICET-UNL), Facultad de Bioquimica 
      y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe 3000, 
      Argentina.
AD  - Max Planck Institute for Developmental Biology, Tubingen D-72076, Germany.
FAU - Yones, Cristian
AU  - Yones C
AD  - Research Institute for Signals, Systems and Computational Intelligence [sinc(i)], 
      Research Institute for Signals, Systems and Computational Intelligence 
      (CONICET-UNL), Ciudad Universitaria, Santa Fe 3000, Argentina.
FAU - Arce, Agustin L
AU  - Arce AL
AUID- ORCID: 0000-0003-3619-3898
AD  - Instituto de Agrobiotecnologia del Litoral (CONICET-UNL), Facultad de Bioquimica 
      y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe 3000, 
      Argentina.
FAU - Stegmayer, Georgina
AU  - Stegmayer G
AD  - Research Institute for Signals, Systems and Computational Intelligence [sinc(i)], 
      Research Institute for Signals, Systems and Computational Intelligence 
      (CONICET-UNL), Ciudad Universitaria, Santa Fe 3000, Argentina.
FAU - Milone, Diego
AU  - Milone D
AD  - Research Institute for Signals, Systems and Computational Intelligence [sinc(i)], 
      Research Institute for Signals, Systems and Computational Intelligence 
      (CONICET-UNL), Ciudad Universitaria, Santa Fe 3000, Argentina.
FAU - Manavella, Pablo A
AU  - Manavella PA
AUID- ORCID: 0000-0003-3589-4414
AD  - Instituto de Agrobiotecnologia del Litoral (CONICET-UNL), Facultad de Bioquimica 
      y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe 3000, Argentina 
      pablomanavella@ial.santafe-conicet.gov.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190301
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (HYL1 protein, Arabidopsis)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SE protein, Arabidopsis)
RN  - EC 3.1.26.3 (DCL1 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Arabidopsis/*metabolism
MH  - Arabidopsis Proteins/*metabolism
MH  - Cell Cycle Proteins/*metabolism
MH  - Cold Temperature
MH  - Computational Biology
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant
MH  - MicroRNAs/*metabolism
MH  - Mutation
MH  - Phenotype
MH  - Pollen/metabolism
MH  - Protein Structure, Secondary
MH  - RNA-Binding Proteins/*metabolism
MH  - Ribonuclease III/*metabolism
MH  - Sequence Analysis, RNA
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - Biogenesis
OT  - HYL1
OT  - Temperature
OT  - microRNA
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2019/02/15 06:00
MHDA- 2019/12/31 06:00
CRDT- 2019/02/15 06:00
PHST- 2018/10/16 00:00 [received]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
AID - dev.172932 [pii]
AID - 10.1242/dev.172932 [doi]
PST - epublish
SO  - Development. 2019 Mar 1;146(5):dev172932. doi: 10.1242/dev.172932.