PMID- 30760619
OWN - NLM
STAT- MEDLINE
DCOM- 20200109
LR  - 20200109
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 202
IP  - 7
DP  - 2019 Apr 1
TI  - Human Blood and Tonsil Plasmacytoid Dendritic Cells Display Similar Gene 
      Expression Profiles but Exhibit Differential Type I IFN Responses to Influenza A 
      Virus Infection.
PG  - 2069-2081
LID - 10.4049/jimmunol.1801191 [doi]
AB  - Influenza A virus (IAV) infection constitutes an annual health burden across the 
      globe. Plasmacytoid dendritic cells (PDCs) are central in antiviral defense 
      because of their superior capacity to produce type I IFNs in response to viruses. 
      Dendritic cells (DCs) differ depending on their anatomical location. However, 
      only limited host-pathogen data are available from the initial site of infection 
      in humans. In this study, we investigated how human tonsil PDCs, likely exposed 
      to virus because of their location, responded to IAV infection compared with 
      peripheral blood PDCs. In tonsils, unlike in blood, PDCs are the most frequent DC 
      subset. Both tonsil and blood PDCs expressed several genes necessary for pathogen 
      recognition and immune response, generally in a similar pattern. MxA, a protein 
      that renders cells resistant to IAV infection, was detected in both tonsil and 
      blood PDCs. However, despite steady-state MxA expression and contrary to previous 
      reports, at high IAV concentrations (typically cytopathic to other immune cells), 
      both tonsil and blood PDCs supported IAV infection. IAV exposure resulted in PDC 
      maturation by upregulation of CD86 expression and IFN-alpha secretion. Interestingly, 
      blood PDCs secreted 10-fold more IFN-alpha in response to IAV compared with tonsil 
      PDCs. Tonsil PDCs also had a dampened cytokine response to purified TLR ligands 
      compared with blood PDCs. Our findings suggest that tonsil PDCs may be less 
      responsive to IAV than blood PDCs, highlighting the importance of studying immune 
      cells at their proposed site of function.
CI  - Copyright (c) 2019 by The American Association of Immunologists, Inc.
FAU - Vangeti, Sindhu
AU  - Vangeti S
AUID- ORCID: 0000-0003-3404-6878
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Gertow, Jens
AU  - Gertow J
AUID- ORCID: 0000-0002-5663-7209
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Yu, Meng
AU  - Yu M
AUID- ORCID: 0000-0002-9798-6624
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Liu, Sang
AU  - Liu S
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Baharom, Faezzah
AU  - Baharom F
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Scholz, Saskia
AU  - Scholz S
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden.
FAU - Friberg, Danielle
AU  - Friberg D
AD  - Department of Surgical Sciences, Uppsala University, 751 85 Uppsala, Sweden.
FAU - Starkhammar, Magnus
AU  - Starkhammar M
AD  - Capio Ear, Nose and Throat Clinic Globen, 121 77 Johanneshov, Sweden; and.
FAU - Ahlberg, Alexander
AU  - Ahlberg A
AUID- ORCID: 0000-0001-5554-912X
AD  - Division of Ear, Nose and Throat Diseases, Department of Clinical Science, 
      Intervention and Technology, Karolinska University Hospital Huddinge, Huddinge, 
      141 86 Stockholm, Sweden.
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
AUID- ORCID: 0000-0001-6966-7039
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet, 171 64 Stockholm, Sweden; anna.smed.sorensen@ki.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190213
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Interferon-alpha)
SB  - IM
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Influenza A virus/immunology
MH  - Influenza, Human/*immunology
MH  - Interferon-alpha/*immunology
MH  - Palatine Tonsil/*immunology
MH  - Transcriptome
EDAT- 2019/02/15 06:00
MHDA- 2020/01/10 06:00
CRDT- 2019/02/15 06:00
PHST- 2018/08/27 00:00 [received]
PHST- 2019/01/25 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2020/01/10 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
AID - jimmunol.1801191 [pii]
AID - 10.4049/jimmunol.1801191 [doi]
PST - ppublish
SO  - J Immunol. 2019 Apr 1;202(7):2069-2081. doi: 10.4049/jimmunol.1801191. Epub 2019 
      Feb 13.