PMID- 30760630
OWN - NLM
STAT- MEDLINE
DCOM- 20200128
LR  - 20200309
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 2
DP  - 2019 Feb 28
TI  - Increase of glandular epithelial cell clusters by an external volume expansion 
      device promotes adipose tissue regeneration by recruiting macrophages.
LID - BSR20181776 [pii]
LID - 10.1042/BSR20181776 [doi]
AB  - Background: There is a clinical need for the use of engineered adipose tissue in 
      place of surgical reconstruction. We previously found that the external volume 
      expansion (EVE) device increased special cell clusters in well-vascularized 
      connective stroma during adipose regeneration. However, the origin of these cell 
      clusters and their role in adipose tissue regeneration remain unknown. Aim: In 
      the present study, we evaluated EVE in the construction of expanded prefabricated 
      adipose tissue (EPAT) in a rat model. Methods: Rats were randomized into an EVE 
      suction group and a control group, with 24 rats in each group. The structure and 
      origin of the special cell clusters were determined by hematoxylin and eosin 
      staining, and immunohistochemistry; their role in adipose tissue regeneration was 
      investigated by immunohistochemistry and Western blot analyses. Results: Special 
      cell clusters began to increase at week 1 with a peak at week 4, and then receded 
      from weeks 8 to 12. Clusters were identified as glandular epithelial cells as 
      determined by their gland-like structure and expression of specific markers. The 
      cell clusters induced significant infiltration of macrophage antigen-2 (Mac-2) 
      positive macrophages by secreting monocyte chemoattractant protein-1 (MCP-1) at 
      the early stage of suction. Subsequently, these infiltrated macrophages expressed 
      massive vascular endothelial growth factor (VEGF) to promoted angiogenesis. 
      Conclusion: EVE generated glandular epithelial cell clusters, which recruited 
      macrophages to promote angiogenesis and subsequent adipose tissue regeneration. 
      These findings shed light on the mechanisms underlying the effects of EVE devices 
      on adipose tissue regeneration.
CI  - (c) 2019 The Author(s).
FAU - Chen, Xihang
AU  - Chen X
AUID- ORCID: 0000-0002-6698-9080
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China.
FAU - He, Yunfan
AU  - He Y
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China.
FAU - Xu, Anqi
AU  - Xu A
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China.
FAU - Deng, Zilong
AU  - Deng Z
AD  - Department of Stomatology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, China.
AD  - College of Stomatology, Southern Medical University, Guangzhou, China.
FAU - Feng, Jingwei
AU  - Feng J
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China.
FAU - Lu, Feng
AU  - Lu F
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China yuanyips@gmail.com doctorlufeng@hotmail.com.
FAU - Yuan, Yi
AU  - Yuan Y
AUID- ORCID: 0000-0003-0446-098X
AD  - Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern 
      Medical University, Guangzhou, China yuanyips@gmail.com doctorlufeng@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190226
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adipose Tissue/blood supply/cytology/*physiology
MH  - Animals
MH  - Epithelial Cells/*cytology/metabolism
MH  - Macrophages/*cytology/metabolism
MH  - Male
MH  - Neovascularization, Physiologic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Regeneration
MH  - *Tissue Expansion Devices
MH  - Vascular Endothelial Growth Factor A/metabolism
PMC - PMC6390125
OTO - NOTNLM
OT  - adipose tissue regeneration
OT  - angiogenesis
OT  - external volume expansion
OT  - glandular epithelial cell clusters
OT  - macrophage
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2019/02/15 06:00
MHDA- 2020/01/29 06:00
PMCR- 2019/02/26
CRDT- 2019/02/15 06:00
PHST- 2018/10/04 00:00 [received]
PHST- 2019/01/27 00:00 [revised]
PHST- 2019/02/07 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2020/01/29 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
PHST- 2019/02/26 00:00 [pmc-release]
AID - BSR20181776 [pii]
AID - 10.1042/BSR20181776 [doi]
PST - epublish
SO  - Biosci Rep. 2019 Feb 26;39(2):BSR20181776. doi: 10.1042/BSR20181776. Print 2019 
      Feb 28.