PMID- 30760775
OWN - NLM
STAT- MEDLINE
DCOM- 20200825
LR  - 20200825
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Feb 13
TI  - Argon reduces the pulmonary vascular tone in rats and humans by GABA-receptor 
      activation.
PG  - 1902
LID - 10.1038/s41598-018-38267-y [doi]
LID - 1902
AB  - Argon exerts neuroprotection. Thus, it might improve patients' neurological 
      outcome after cerebral disorders or cardiopulmonary resuscitation. However, 
      limited data are available concerning its effect on pulmonary vessel and airways. 
      We used rat isolated perfused lungs (IPL) and precision-cut lung slices (PCLS) of 
      rats and humans to assess this topic. IPL: Airway and perfusion parameters, 
      oedema formation and the pulmonary capillary pressure (P(cap)) were measured and 
      the precapillary and postcapillary resistance (R(post)) was calculated. In IPLs 
      and PCLS, the pulmonary vessel tone was enhanced with ET-1 or remained unchanged. 
      IPLs were ventilated and PCLS were gassed with argon-mixture or room-air. IPL: 
      Argon reduced the ET-1-induced increase of P(cap), R(post) and oedema formation 
      (p < 0.05). PCLS (rat): Argon relaxed naive pulmonary arteries (PAs) (p < 0.05). 
      PCLS (rat/human): Argon attenuated the ET-1-induced contraction in PAs 
      (p < 0.05). Inhibition of GABA(B)-receptors abolished argon-induced relaxation 
      (p < 0.05) in naive or ET-1-pre-contracted PAs; whereas inhibition of 
      GABA(A)-receptors only affected ET-1-pre-contracted PAs (p < 0.01). 
      GABA(A/B)-receptor agonists attenuated ET-1-induced contraction in PAs and 
      baclofen (GABA(B)-agonist) even in pulmonary veins (p < 0.001). PLCS (rat): Argon 
      did not affect the airways. Finally, argon decreases the pulmonary vessel tone by 
      activation of GABA-receptors. Hence, argon might be applicable in patients with 
      pulmonary hypertension and right ventricular failure.
FAU - Suleiman, Said
AU  - Suleiman S
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Klassen, Sergej
AU  - Klassen S
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Katz, Ira
AU  - Katz I
AD  - Medical Research & Development, Air Liquide Sante Internationale, Centre de 
      Recherche Paris-Saclay, 78354, Jouy-en-Josas, France.
FAU - Balakirski, Galina
AU  - Balakirski G
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Krabbe, Julia
AU  - Krabbe J
AUID- ORCID: 0000-0003-4205-9783
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - von Stillfried, Saskia
AU  - von Stillfried S
AD  - Institute of Pathology, Medical Faculty RWTH Aachen, 52074, Aachen, Germany.
FAU - Kintsler, Svetlana
AU  - Kintsler S
AD  - Institute of Pathology, Medical Faculty RWTH Aachen, 52074, Aachen, Germany.
FAU - Braunschweig, Till
AU  - Braunschweig T
AD  - Institute of Pathology, Medical Faculty RWTH Aachen, 52074, Aachen, Germany.
FAU - Babendreyer, Aaron
AU  - Babendreyer A
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Spillner, Jan
AU  - Spillner J
AD  - Department of Cardiac and Thoracic Surgery, Medical Faculty RWTH Aachen, 52074, 
      Aachen, Germany.
FAU - Kalverkamp, Sebastian
AU  - Kalverkamp S
AD  - Department of Cardiac and Thoracic Surgery, Medical Faculty RWTH Aachen, 52074, 
      Aachen, Germany.
FAU - Schroder, Thomas
AU  - Schroder T
AD  - Department of Surgery, Luisenhospital Aachen, 52064, Aachen, Germany.
FAU - Moeller, Manfred
AU  - Moeller M
AD  - Institute for Occupational, Social and Environmental Medicine, Medical Faculty 
      RWTH Aachen, 52074, Aachen, Germany.
FAU - Coburn, Mark
AU  - Coburn M
AD  - Department of Anaesthesiology, Medical Faculty RWTH Aachen, 52074, Aachen, 
      Germany.
FAU - Uhlig, Stefan
AU  - Uhlig S
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Martin, Christian
AU  - Martin C
AD  - Institute of Pharmacology and Toxicology, Medical Faculty RWTH-Aachen, 52074, 
      Aachen, Germany.
FAU - Rieg, Annette D
AU  - Rieg AD
AUID- ORCID: 0000-0002-7043-5494
AD  - Department of Anaesthesiology, Medical Faculty RWTH Aachen, 52074, Aachen, 
      Germany. arieg@ukaachen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190213
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Endothelin-1)
RN  - 0 (GABA-B Receptor Agonists)
RN  - 0 (Receptors, GABA-B)
RN  - 67XQY1V3KH (Argon)
RN  - H789N3FKE8 (Baclofen)
SB  - IM
MH  - Animals
MH  - Argon/*pharmacology
MH  - Baclofen/pharmacology
MH  - Blood Pressure/drug effects
MH  - Edema/chemically induced/prevention & control
MH  - Endothelin-1/pharmacology
MH  - Female
MH  - GABA-B Receptor Agonists/pharmacology
MH  - Hemodynamics/drug effects
MH  - Humans
MH  - Lung/pathology/physiology
MH  - Muscle Contraction/drug effects
MH  - Pulmonary Artery/*drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, GABA-B/chemistry/*metabolism
PMC - PMC6374423
COIS- Said Suleiman has no financial and non-financial interests. Sergej Klassen has no 
      financial and non-financial interests. Ira Katz works for Air Liquide. Even he 
      could have a commercial interest; he did not influence the design or the outcome 
      of the study. Ira Katz was very helpful with his experience in view of 
      physiochemical properties of laboratory gases to explain and interpret some 
      unexpected effects of argon on the airway parameters. Galina Balakirski has no 
      financial and non-financial interests. Julia Krabbe has no financial and 
      non-financial interests. Saskia von Stillfried has no financial and non-financial 
      interests. Svetlana Kintsler has no financial and non-financial interests. Till 
      Braunschweig has no financial and non-financial interests. Aaron Babendreyer has 
      no financial and non-financial interests. Jan Spillner has no financial and 
      non-financial interests. Sebastian Kalverkamp has no financial and non-financial 
      interests. Thomas Schroder has no financial and non-financial interests. Manfred 
      Moeller has no financial and non-financial interests. Mark Coburn has no 
      financial and non-financial interests. Stefan Uhlig has no financial and 
      non-financial interests. Christian Martin has no financial and non-financial 
      interests. Annette D Rieg has no financial and non-financial interests.
EDAT- 2019/02/15 06:00
MHDA- 2020/08/26 06:00
PMCR- 2019/02/13
CRDT- 2019/02/15 06:00
PHST- 2018/09/12 00:00 [received]
PHST- 2018/12/21 00:00 [accepted]
PHST- 2019/02/15 06:00 [entrez]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2020/08/26 06:00 [medline]
PHST- 2019/02/13 00:00 [pmc-release]
AID - 10.1038/s41598-018-38267-y [pii]
AID - 38267 [pii]
AID - 10.1038/s41598-018-38267-y [doi]
PST - epublish
SO  - Sci Rep. 2019 Feb 13;9(1):1902. doi: 10.1038/s41598-018-38267-y.