PMID- 30761631
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20190906
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 103
IP  - 3
DP  - 2019 May
TI  - Leucine-induced promotion of post-absorptive EAA utilization and hepatic 
      gluconeogenesis contributes to protein synthesis in skeletal muscle of dairy 
      calves.
PG  - 705-712
LID - 10.1111/jpn.13072 [doi]
AB  - The high rate of protein synthesis in skeletal muscle of dairy calves can benefit 
      their first lactation even lifetime milk yield. Since the rate of protein 
      synthesis is relatively low in the post-absorptive state, the aim of this 
      research was to determine whether leucine supplementation could increase the 
      post-absorptive essential amino acid (EAA) utilization and protein synthesis in 
      the skeletal muscle. Ten male neonatal dairy calves (38 +/- 3 kg) were randomly 
      assigned to either the control (CON, no leucine supplementation, n = 5) or 
      supplementation with 1.435 g leucine/L milk (LEU, n = 5). Results showed that 
      leucine significantly increased the length and protein concentration in 
      longissimus dorsi (LD) muscle, whereas it decreased creatinine concentration and 
      glutamic-oxalacetic transaminase (GOT) activity. Compared to the control group, 
      leucine supplementation also reduced the glutamic-pyruvic transaminase (GPT) 
      activity. Supplementation of leucine improved the phosphorylation of mammalian 
      target of rapamycin (mTOR), eukaryotic initiation factor 4E-binding protein 1 
      (4EBP1) and substrates ribosomal protein S6 kinase 1 (p70S6K). Supplementation of 
      leucine resulted in increased concentrations of glucose, methionine, threonine, 
      histidine and EAAs and decreased concentration of arginine in serum. Liver 
      glucose concentration was higher and pyranic acid was lower in LEU compared to 
      CON. In conclusion, leucine supplementation can promote post-absorptive EAA 
      utilization and hepatic gluconeogenesis, which contributes to protein synthesis 
      in skeletal muscle of dairy calves.
CI  - (c) 2019 Blackwell Verlag GmbH.
FAU - Zheng, Chen
AU  - Zheng C
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Yao, Junhu
AU  - Yao J
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Guo, Long
AU  - Guo L
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Cao, Yangchun
AU  - Cao Y
AUID- ORCID: 0000-0003-1033-2909
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Liang, Ziqi
AU  - Liang Z
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Yang, Xinjian
AU  - Yang X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
FAU - Cai, Chuanjiang
AU  - Cai C
AUID- ORCID: 0000-0002-2824-8329
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      China.
LA  - eng
GR  - 31472122/National Natural Science Foundation of China/
GR  - 31672451/National Natural Science Foundation of China/
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20190213
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (Amino Acids, Essential)
RN  - 0 (Muscle Proteins)
RN  - GMW67QNF9C (Leucine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Amino Acids, Essential/*metabolism
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Cattle
MH  - Diet/veterinary
MH  - Dietary Supplements
MH  - Gluconeogenesis/*physiology
MH  - Glucose/metabolism
MH  - Leucine/*pharmacology
MH  - Liver/*drug effects/metabolism
MH  - Male
MH  - Muscle Proteins/*biosynthesis
MH  - Muscle, Skeletal/*metabolism
MH  - Random Allocation
OTO - NOTNLM
OT  - amino acid metabolism
OT  - dairy calves
OT  - leucine
OT  - protein synthesis
OT  - skeletal muscle
EDAT- 2019/02/15 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/02/15 06:00
PHST- 2018/10/09 00:00 [received]
PHST- 2018/12/07 00:00 [revised]
PHST- 2019/01/15 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
AID - 10.1111/jpn.13072 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2019 May;103(3):705-712. doi: 10.1111/jpn.13072. 
      Epub 2019 Feb 13.