PMID- 30761780
OWN - NLM
STAT- MEDLINE
DCOM- 20190829
LR  - 20190829
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 47
IP  - 6
DP  - 2019 Jun
TI  - Image quantification technology of the heterochromatin and euchromatin region for 
      differential diagnosis in the lobular endocervical glandular hyperplasia.
PG  - 553-563
LID - 10.1002/dc.24155 [doi]
AB  - BACKGROUND: Lobular endocervical glandular hyperplasia (LEGH) was first described 
      by Nucci et al. in 1999 and is believed to be a precancerous lesion of minimal 
      deviation adenocarcinoma and gastric-type adenocarcinoma in the uterine cervix. 
      LEGH lesions do not always exhibit apparent cellular and structural atypia, so 
      are difficult to distinguish from normal endocervical cells (EC cells) with 
      cytological examination. Therefore, we often struggle to make a definite 
      diagnosis of LEGH. METHODS: We used microscopy images of cytological specimens 
      that were diagnosed as EC cells and LEGH cells. Signal intensity in whole nuclear 
      area and in heterochromatin and euchromatin regions, euchromatin area ratio, and 
      nuclear morphological features were quantified in each cell nucleus of the cases. 
      Statistical analyses were conducted to determine statistical significance. 
      Finally, we performed linear support vector machine (LSVM) modeling as a 
      discriminant analysis using the quantified features. RESULTS: Signal intensity in 
      whole nuclear area, and heterochromatin and euchromatin regions of EC cell nuclei 
      were higher than that of the LEGH cell nuclei. Morphologically, EC cell nuclei 
      were larger than LEGH cell nuclei, and nuclei of LEGH cells had irregular nuclear 
      respectively membrane structure and an elongated shape. The LSVM accuracy of 
      10-fold cross validation and leave-one-case-out cross-validation (LOCOCV) using 
      all measured features were 84.7% to 89.3% and 78.6% to 86.0%, respectively. 
      CONCLUSIONS: The LVSM analysis using features extracted from signal intensity and 
      morphological analysis was useful for discrimination of EC cells vs LEGH cells. 
      We therefore believe that this image analysis method could be used for early 
      detection of LEGH.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Kimura, Fumikazu
AU  - Kimura F
AUID- ORCID: 0000-0001-7269-2497
AD  - Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu 
      University.
FAU - Kobayashi, Takaki
AU  - Kobayashi T
AD  - Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu 
      University.
FAU - Kanai, Ryo
AU  - Kanai R
AD  - Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu 
      University.
FAU - Kobayashi, Yukihiro
AU  - Kobayashi Y
AD  - Department of Laboratory Medicine, Shinshu University Hospital.
FAU - Yuhi, Ohtani
AU  - Yuhi O
AD  - Department of Information Processing, Interdisciplinary Graduate School of 
      Science and Engineering, Tokyo Institute of Technology.
FAU - Ota, Hiroyoshi
AU  - Ota H
AD  - Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu 
      University.
FAU - Yamaguchi, Masahiro
AU  - Yamaguchi M
AD  - Department of Information Processing, Interdisciplinary Graduate School of 
      Science and Engineering, Tokyo Institute of Technology.
FAU - Yokokawa, Yoshiharu
AU  - Yokokawa Y
AD  - Division of Physical Therapy, Shinshu University School of Health Sciences.
FAU - Uehara, Takeshi
AU  - Uehara T
AD  - Department of Laboratory Medicine, Shinshu University School of Medicine.
FAU - Ishii, Keiko
AU  - Ishii K
AD  - Division of Diagnostic Pathology, Okaya City Hospital.
LA  - eng
PT  - Journal Article
DEP - 20190213
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (Euchromatin)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Cell Nucleus/pathology
MH  - Diagnosis, Differential
MH  - Euchromatin/*chemistry
MH  - Female
MH  - Heterochromatin/*chemistry
MH  - Humans
MH  - Hyperplasia
MH  - *Image Processing, Computer-Assisted
MH  - Reproducibility of Results
MH  - Uterine Cervical Neoplasms/*diagnosis/*pathology
OTO - NOTNLM
OT  - euchromatin
OT  - heterochromatin
OT  - linear support vector machine
OT  - lobular endocervical glandular hyperplasia
OT  - signal intensity
EDAT- 2019/02/15 06:00
MHDA- 2019/08/30 06:00
CRDT- 2019/02/15 06:00
PHST- 2018/09/07 00:00 [received]
PHST- 2019/01/18 00:00 [revised]
PHST- 2019/01/22 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2019/08/30 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
AID - 10.1002/dc.24155 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2019 Jun;47(6):553-563. doi: 10.1002/dc.24155. Epub 2019 Feb 
      13.