PMID- 30763279
OWN - NLM
STAT- MEDLINE
DCOM- 20200501
LR  - 20200501
IS  - 1741-7899 (Electronic)
IS  - 1470-1626 (Linking)
VI  - 157
IP  - 5
DP  - 2019 May
TI  - Serum and tissue pregnanes and pregnenes after dexamethasone treatment of cows in 
      late gestation.
PG  - 413-422
LID - 10.1530/REP-18-0558 [doi]
AB  - Dexamethasone (DEX) initiates parturition by inducing progesterone withdrawal and 
      affecting placental steroidogenesis, but the effects of DEX in fetal and maternal 
      tissue steroid synthetic capacity remains poorly investigated. Blood was 
      collected from cows at 270 days of gestation before DEX or saline (SAL) 
      treatment, and blood and tissues were collected at slaughter 38 h later. Steroid 
      concentrations were determined by liquid chromatography tandem mass spectrometry 
      to detect multiple steroids including 5alpha-reduced pregnane metabolites of 
      progesterone. The activities of 3beta-hydroxysteroid dehydrogenase (3betaHSD) in 
      cotyledonary and luteal microsomes and mitochondria and cotyledonary microsomal 
      5alpha-reductase were assessed. Quantitative PCR was used to further assess 
      transcripts encoding enzymes and factors supporting steroidogenesis in 
      cotyledonary and luteal tissues. Serum progesterone, pregnenolone, 
      5alpha-dihydroprogesterone (DHP) and allopregnanolone (3alphaDHP) concentrations (all <5 
      ng/mL before treatment) decreased in cows after DEX. However, the 
      20alpha-hydroxylated metabolite of DHP, 20alphaDHP, was higher before treatment ( approximately 100 
      ng/mL) than at slaughter but not affected by DEX. Serum, cotyledonary and luteal 
      progesterone was lower in DEX- than SAL-treated cows. Progesterone was >100-fold 
      higher in luteal than cotyledonary tissues, and serum and luteal concentrations 
      were highly correlated in DEX-treated cows. 3betaHSD activity was >5-fold higher in 
      luteal than cotyledonary tissue, microsomes had more 3betaHSD than mitochondria in 
      luteal tissue but equal in cotyledonary sub-cellular fractions. DEX did not 
      affect either luteal or cotyledonary 3betaHSD activity but luteal steroidogenic 
      enzyme transcripts were lower in DEX-treated cows. DEX induced functional luteal 
      regression and progesterone withdrawal before any changes in placental 
      pregnene/pregnane synthesis and/or metabolism were detectable.
FAU - Conley, Alan J
AU  - Conley AJ
AD  - Department of Population Health & Reproduction, University of California, Davis, 
      California, USA.
FAU - Legacki, Erin L
AU  - Legacki EL
AD  - Department of Population Health & Reproduction, University of California, Davis, 
      California, USA.
FAU - Corbin, C Jo
AU  - Corbin CJ
AD  - Department of Population Health & Reproduction, University of California, Davis, 
      California, USA.
FAU - Stanley, Scott
AU  - Stanley S
AD  - Department of Molecular Biosciences, School of Veterinary Medicine, University of 
      California, Davis, California, USA.
FAU - Dahlen, Carl R
AU  - Dahlen CR
AD  - Center for Nutrition and Pregnancy and Department of Animal Sciences, North 
      Dakota State University, Fargo, North Dakota, USA.
FAU - Reynolds, Lawrence P
AU  - Reynolds LP
AD  - Center for Nutrition and Pregnancy and Department of Animal Sciences, North 
      Dakota State University, Fargo, North Dakota, USA.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Pregnanes)
RN  - 0 (Pregnenes)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - *Cattle/metabolism
MH  - Corpus Luteum/drug effects/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Female
MH  - Fetus/drug effects/metabolism
MH  - Gestational Age
MH  - Luteolysis/blood/drug effects/metabolism
MH  - Parturition/*drug effects/metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal/blood/drug effects/metabolism
MH  - Pregnanes/blood/*metabolism
MH  - Pregnenes/blood/*metabolism
MH  - Progesterone/metabolism
EDAT- 2019/02/15 06:00
MHDA- 2020/05/02 06:00
CRDT- 2019/02/15 06:00
PHST- 2018/10/26 00:00 [received]
PHST- 2019/02/14 00:00 [accepted]
PHST- 2019/02/15 06:00 [pubmed]
PHST- 2020/05/02 06:00 [medline]
PHST- 2019/02/15 06:00 [entrez]
AID - REP-18-0558.R2 [pii]
AID - 10.1530/REP-18-0558 [doi]
PST - ppublish
SO  - Reproduction. 2019 May;157(5):413-422. doi: 10.1530/REP-18-0558.