PMID- 30767578 OWN - NLM STAT- MEDLINE DCOM- 20190321 LR - 20200225 IS - 2058-7384 (Electronic) IS - 0394-6320 (Print) IS - 0394-6320 (Linking) VI - 33 DP - 2019 Jan-Dec TI - Heat stress combined with lipopolysaccharide alter the activity and superficial molecules of peripheral monocytes. PG - 2058738419828891 LID - 10.1177/2058738419828891 [doi] LID - 2058738419828891 AB - The purpose of this study was to focus on the underlying relationship between the hyperactivity for the peripheral monocytes and heat stroke by investigating the inflammatory oxidative activity of and the expression of superficial molecules. Peripheral blood samples were collected from 10 healthy adult volunteers. Human blood monocytes were isolated by density gradient centrifugation and sequent adherent culture. The objectives were divided into four groups: 43 degrees C heat stress combined with lipopolysaccharide (LPS) group, 43 degrees C heat stress group, LPS group, and control group. There were 10 cases in each group. An enzyme-linked immunosorbent assay (ELISA) test was used to measure the concentrations of supernatant inflammatory mediators (tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-10 (IL-10)). After loaded by 2,7-Dichlorodi-hydrofluorescein-diacetate (DCFHDA) fluorescent probe, intracellular reactive oxygen species (ROS) levels were determined by a flow cytometry. After fluorescent microspheres incubation, the phagocytosis of monocytes was observed under a fluorescent microscope. Respectively, the flow cytometry and Western blot were used to evaluate the level of triggering receptor expressed on myeloid cells-1 (TREM-1) and Toll-like receptor-4 (TLR-4) on the monocytes. Furthermore, the mRNA expression of TREM-1 and TLR-4 was detected by real-time polymerase chain reaction (RT-PCR). The heat stress combined with LPS stimulation promoted the peripheral monocytes to produce inflammatory mediators (TNF-alpha, IL-1beta, and IL-10) and release ROS. Otherwise, such complex strike significantly suppressed the phagocytic activity of monocytes in peripheral blood. Moreover, the expression of TREM-1, TLR-4 and CD86 was measured by the flow cytometry on peripheral monocytes which were respectively promoted by the union of heat stress and LPS. The results of Western blot and RT-PCR demonstrated the similar kinetics on these superficial molecules (TREM-1, TLR-4, and CD86) stimulated by the combination of heat stress and LPS. The underlying mechanism of the dysfunction for the peripheral monocytes may be related to the abnormal expression of superficial molecules TREM-1, TLR-4, and CD86 on the monocytes induced by heat stress and LPS. FAU - Luo, Jiajing AU - Luo J AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Chen, Yi AU - Chen Y AUID- ORCID: 0000-0002-3811-3460 AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Ding, Chengjia AU - Ding C AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Qiu, Jialing AU - Qiu J AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Chen, Yulan AU - Chen Y AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Lin, Youping AU - Lin Y AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. FAU - Su, Lei AU - Su L AD - 2 Department of Critical Care Medicine, General Hospital of Southern Theater Command, Key Laboratory of Tropical Trauma Care and Tissue Repair of PLA, Guangzhou, China. FAU - Jiang, Dongxin AU - Jiang D AD - 1 Department of Critical Care Medicine, The Fifth Peoples' Hospital of Dongguan, Dongguan Hospital Affiliated to Jinan University, Dongguan, China. LA - eng PT - Journal Article PL - England TA - Int J Immunopathol Pharmacol JT - International journal of immunopathology and pharmacology JID - 8911335 RN - 0 (B7-2 Antigen) RN - 0 (Interleukin-1beta) RN - 0 (Lipopolysaccharides) RN - 0 (Membrane Glycoproteins) RN - 0 (RNA, Messenger) RN - 0 (Toll-Like Receptor 1) RN - 0 (Triggering Receptor Expressed on Myeloid Cells-1) RN - 0 (Tumor Necrosis Factor-alpha) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Adult MH - B7-2 Antigen/metabolism MH - Heat-Shock Response/*physiology MH - Humans MH - Interleukin-10/metabolism MH - Interleukin-1beta/metabolism MH - Leukocytes, Mononuclear/drug effects/metabolism MH - Lipopolysaccharides/*pharmacology MH - Male MH - Membrane Glycoproteins/metabolism MH - Monocytes/*drug effects/metabolism MH - Phagocytes/drug effects/metabolism MH - RNA, Messenger/metabolism MH - Toll-Like Receptor 1/metabolism MH - Triggering Receptor Expressed on Myeloid Cells-1/metabolism MH - Tumor Necrosis Factor-alpha/metabolism MH - Young Adult PMC - PMC6378461 OTO - NOTNLM OT - CD86 OT - TLR-4 OT - TREM-1 OT - heat stroke OT - monocytes COIS- Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2019/02/16 06:00 MHDA- 2019/03/22 06:00 PMCR- 2019/02/15 CRDT- 2019/02/16 06:00 PHST- 2019/02/16 06:00 [entrez] PHST- 2019/02/16 06:00 [pubmed] PHST- 2019/03/22 06:00 [medline] PHST- 2019/02/15 00:00 [pmc-release] AID - 10.1177_2058738419828891 [pii] AID - 10.1177/2058738419828891 [doi] PST - ppublish SO - Int J Immunopathol Pharmacol. 2019 Jan-Dec;33:2058738419828891. doi: 10.1177/2058738419828891.