PMID- 30767702
OWN - NLM
STAT- MEDLINE
DCOM- 20190422
LR  - 20190422
IS  - 1532-2297 (Electronic)
IS  - 1082-6068 (Linking)
VI  - 49
IP  - 3
DP  - 2019
TI  - Novel attacin from Hermetia illucens: cDNA cloning, characterization, and 
      antibacterial properties.
PG  - 279-285
LID - 10.1080/10826068.2018.1541807 [doi]
AB  - In this study, we report a novel member of the attacin family from Hermetia 
      illucens. The cDNA clone encoding the attacin-like protein was isolated by 
      screening a cDNA library prepared from immunized fat body. The complete 510 bp 
      cDNA of HI-attacin was predicted to encode a protein of 169 amino acids with a 
      molecular weight of 17.7 kDa. The putative mature protein of H. illucens attacin 
      (HI-attacin) had 50% identity with that of Bactrocera dorsalis attacin B. 
      Phylogenetic analysis revealed that the HI-attacin was separated from the other 
      dipteran attacins with a high bootstrap percentage. Compared to that in the other 
      dipteran attacins, the G1 domain of HI-attacin was shorter and the sequence of 
      the G2 domain of HI-attacin was more conserved than that of the G1 domain. We 
      produced the recombinant attacin (rHI-attacin) protein using a prokaryotic 
      expression system to confirm its antibacterial character. The rHI-attacin was 
      produced as inclusion bodies and refolded by on-column refolding. rHI-attacin 
      exhibited antibacterial activity against both Escherichia coli and 
      methicillin-resistant Staphylococcus aureus (MRSA). Using real-time PCR, the 
      expression of HI-attacin was was barely detected before the immunization, but was 
      mostly evident in the fat body after immunization.
FAU - Shin, Hak Sup
AU  - Shin HS
AD  - a Department of Biological Sciences , Dankook University , Cheonan , Republic of 
      Korea.
FAU - Park, Soon-Ik
AU  - Park SI
AD  - a Department of Biological Sciences , Dankook University , Cheonan , Republic of 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20190215
PL  - England
TA  - Prep Biochem Biotechnol
JT  - Preparative biochemistry & biotechnology
JID - 9607037
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Insect Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (attacin antibacterial protein, insect)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/isolation & purification/*pharmacology
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Diptera/*chemistry
MH  - Escherichia coli/drug effects
MH  - Fat Body/chemistry
MH  - Gene Expression
MH  - Insect Proteins/chemistry/genetics/isolation & purification/*pharmacology
MH  - Methicillin-Resistant Staphylococcus aureus/drug effects
MH  - Phylogeny
MH  - RNA/isolation & purification
MH  - Recombinant Proteins/chemistry/genetics/isolation & purification/pharmacology
MH  - Sequence Alignment
OTO - NOTNLM
OT  - Animal biotechnology
OT  - characterization
OT  - chromatography
OT  - refolding
EDAT- 2019/02/16 06:00
MHDA- 2019/04/23 06:00
CRDT- 2019/02/16 06:00
PHST- 2019/02/16 06:00 [pubmed]
PHST- 2019/04/23 06:00 [medline]
PHST- 2019/02/16 06:00 [entrez]
AID - 10.1080/10826068.2018.1541807 [doi]
PST - ppublish
SO  - Prep Biochem Biotechnol. 2019;49(3):279-285. doi: 10.1080/10826068.2018.1541807. 
      Epub 2019 Feb 15.