PMID- 30769432
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 92
IP  - 9
DP  - 2008 Sep
TI  - Natural Infection of Swinglea glutinosa by the Citrus leprosis virus Cytoplasmic 
      Type (CiLV-C) in Colombia.
PG  - 1364
LID - 10.1094/PDIS-92-9-1364C [doi]
AB  - Swinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is 
      originally from southeast Asia but which is now grown worldwide. In Colombia, it 
      is used as an ornamental and principally as a living fence around rural 
      properties and farms in several regions of the country. Citrus leprosis virus 
      cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian 
      Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of 
      the potential for country-wide infection of citrus, some measures are being taken 
      to avoid CiLV-C spread to other regions of Colombia. Further surveys made from 
      June to December 2005 to evaluate the extent of the spread of CiLV-C in the 
      Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus 
      orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, 
      similar to those caused by CiLV-C on sweet oranges leaves. These plants were 
      found near citrus orchards in the municipalities of Guamal and in some urban 
      areas of Villavicencio City in the Meta Department. The possibility that these 
      symptoms were caused by CiLV-C was investigated soon after sample collection by 
      the same procedures as described previously for sweet orange (2). In the leaf 
      lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic 
      viroplasm were found with electron microscopy. Total RNA extracted from 
      symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers 
      (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically 
      amplify a region within the CiLV-C putative methyltransferase gene and this 
      yielded a single fragment of the expected 402 bp (3). Analysis of the consensus 
      sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 
      96% nucleotide and 92% amino acid sequence identity to the sequence of a 
      Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), 
      respectively. Recently, published work described mite transmission of CiLV-C to 
      some nonrutaceous plants (1), but to our knowledge, this is the first report of a 
      nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus 
      orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which 
      are likely the most important vector of CiLV-C in citrus in Colombia, were 
      observed feeding on healthy and symptomatic S. glutinosa, indicating that S. 
      glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living 
      fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa 
      plants may play a role in the epidemiology of leprosis in commercial citrus by 
      serving as an inoculum source for this lethal virus. References: (1) M. Bastianel 
      et al. Summa Phytopathol. 32:211, 2006. (2) G. A. Leon et al. Plant Dis. 90:682, 
      2006. (3) E. C. Locali et al. Plant Dis. 87:1317, 2003.
FAU - Leon, M G
AU  - Leon MG
AD  - Centro de Investigacion La Libertad, CORPOICA, Villavicencio, Colombia.
FAU - Becerra, C H
AU  - Becerra CH
AD  - ICA, Seccional Villavicencio, Colombia.
FAU - Freitas-Astua, J
AU  - Freitas-Astua J
AD  - Embrapa Cassava and Tropical Fruits and CAPTASM-IAC, CP 4, 13490-970 
      Cordeiropolis, SP, Brazil.
FAU - Salaroli, R B
AU  - Salaroli RB
AD  - Departamento de Entomologia, Fitopatologia e Zoologia Agricola, ESALQ, CP 9, 
      13418-900 Piracicaba, SP, Brazil.
FAU - Kitajima, E W
AU  - Kitajima EW
AD  - Departamento de Entomologia, Fitopatologia e Zoologia Agricola, ESALQ, CP 9, 
      13418-900 Piracicaba, SP, Brazil.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
EDAT- 2008/09/01 00:00
MHDA- 2008/09/01 00:01
CRDT- 2019/02/17 06:00
PHST- 2019/02/17 06:00 [entrez]
PHST- 2008/09/01 00:00 [pubmed]
PHST- 2008/09/01 00:01 [medline]
AID - 10.1094/PDIS-92-9-1364C [doi]
PST - ppublish
SO  - Plant Dis. 2008 Sep;92(9):1364. doi: 10.1094/PDIS-92-9-1364C.