PMID- 30770986
OWN - NLM
STAT- MEDLINE
DCOM- 20190709
LR  - 20200225
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 474
IP  - 6
DP  - 2019 Jun
TI  - The stem cell markers expression CD44v6 and podoplanin in lip cancer: clinical 
      significance.
PG  - 745-754
LID - 10.1007/s00428-019-02539-3 [doi]
AB  - This study aimed to analyze the immunoexpression of cancer stem cell markers, 
      CD44v6, and podoplanin in 91 patients with lip squamous cell carcinomas (LSCC). 
      The immunostaining of podoplanin and CD44v6 was evaluated in ten high-power 
      fields (x 400 magnification) at the invasive front of LSCC, using a 
      semi-quantitative score method. Chi-square test or Fisher's exact test was used 
      to verify the association of podoplanin and CD44v6 expressions with 
      clinicopathologic variables. Spearman's correlation test was used to analyze the 
      correlation between the two antibodies in lip cancer. Disease-free survival 
      probabilities in 5 and 10 years were estimated according to the Kaplan-Meier 
      method and compared using the log-rank test. The independent effects of the 
      significant variables were analyzed by Cox proportional hazards regression model. 
      A strong podoplanin expression was observed in the membrane and cytoplasm of most 
      lip tumor cells, and this was inversely associated with locoregional recurrence 
      (p = 0.028) and with histopathological grade of malignancy (p = 0.026). 
      Additionally, CD44v6 immunostaining was strongly expressed in the membrane of 
      tumor cells in 95.4% of the LSCC. Patients with strong membranous (p = 0.016) or 
      strong cytoplasmic (p = 0.030) podoplanin-positive tumors resulted in 
      significantly better disease-free survival than those who had podoplanin 
      weak/negative tumors, confirming podoplanin expression as a favorable independent 
      prognostic factor. Podoplanin and CD44v6 were strongly expressed by tumor cells 
      and podoplanin immunoexpression can help to determine lip cancer patients with 
      lower risk for disease recurrence.
FAU - Garcia, Alexandre Simoes
AU  - Garcia AS
AD  - Department of Surgery, Stomatology, Pathology and Radiology, Area of Pathology, 
      Bauru School of Dentistry, University of Sao Paulo, Alameda Octavio Pinheiro 
      Brisola, 9-75, Bauru, SP, 17012-901, Brazil.
FAU - Assao, Agnes
AU  - Assao A
AD  - Department of Surgery, Stomatology, Pathology and Radiology, Area of Pathology, 
      Bauru School of Dentistry, University of Sao Paulo, Alameda Octavio Pinheiro 
      Brisola, 9-75, Bauru, SP, 17012-901, Brazil.
FAU - Carvalho, Andre Lopes
AU  - Carvalho AL
AD  - Fundacao Pio XII Institution - Cancer Hospital of Barretos, Brazil, R. Antenor 
      Duarte Vilela, 1331, Barretos, SP, 14784-400, Brazil.
FAU - Soares, Fernando Augusto
AU  - Soares FA
AD  - Rede D'Or Hospitals Network - Pathology Division - Brazil, R. das Perobas, 344, 
      Jabaquara, Sao Paulo, SP, 04321-120, Brazil.
FAU - Kowalski, Luiz Paulo
AU  - Kowalski LP
AD  - Department of Head and Neck Surgery and Otorhinolaryngology, A.C. Camargo Cancer 
      Hospital, R. Professor Antonio Prudente, 211, Liberdade, Sao Paulo, SP, 
      01509-010, Brazil.
FAU - Oliveira, Denise Tostes
AU  - Oliveira DT
AUID- ORCID: 0000-0002-4628-7129
AD  - Department of Surgery, Stomatology, Pathology and Radiology, Area of Pathology, 
      Bauru School of Dentistry, University of Sao Paulo, Alameda Octavio Pinheiro 
      Brisola, 9-75, Bauru, SP, 17012-901, Brazil. denisetostes@usp.br.
LA  - eng
GR  - #2012/13411-6/Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo/
GR  - 001/Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior/
PT  - Journal Article
DEP - 20190215
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (PDPN protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Squamous Cell/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Hyaluronan Receptors/*metabolism
MH  - Lip Neoplasms/*metabolism/pathology
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/metabolism/pathology
MH  - Stem Cells/*metabolism
OTO - NOTNLM
OT  - Lip
OT  - Podoplanin
OT  - Squamous cell carcinoma
OT  - cd44 antigens
EDAT- 2019/02/17 06:00
MHDA- 2019/07/10 06:00
CRDT- 2019/02/17 06:00
PHST- 2018/11/21 00:00 [received]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2019/01/09 00:00 [revised]
PHST- 2019/02/17 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PHST- 2019/02/17 06:00 [entrez]
AID - 10.1007/s00428-019-02539-3 [pii]
AID - 10.1007/s00428-019-02539-3 [doi]
PST - ppublish
SO  - Virchows Arch. 2019 Jun;474(6):745-754. doi: 10.1007/s00428-019-02539-3. Epub 
      2019 Feb 15.