PMID- 30771952
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 197
DP  - 2019 May 15
TI  - Rapid and sensitive capillary electrophoresis method for the analysis of Ecstasy 
      in an oral fluid.
PG  - 390-396
LID - S0039-9140(19)30029-3 [pii]
LID - 10.1016/j.talanta.2019.01.029 [doi]
AB  - In the present study, a capillary electrophoresis method, with a native 
      fluorescence detection for the quantification of three amphetamine derivatives, 
      methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), 
      methylenedioxyethylamphetamine (MDEA) in an oral fluid is described. The reported 
      CE method has made it possible to assess Ecstasy abuse in approximately 15 min, 
      including a saliva sample collection, pretreatment procedures and capillary 
      electrophoresis (CE) analysis. The proof of the principle that was demonstrated 
      on a home-made lab scale instrument has had the potential to be easily translated 
      onto a truly portable instrument for on-site measurements. The baseline CE 
      separation of the three illegal drugs was achieved in 10 min, by applying an 
      aqueous background electrolyte (BGE) that was composed of 40 mM phosphoric acid 
      and 10 mM triethanolamine. The amphetamine derivatives were detected at their 
      lambda(ex)/lambda(em) maximum (280/326 nm) with LOD values of about 3 ng/mL for each 
      amphetamine. The recovery of the compounds from the collection pad was about 40% 
      of the LOQ concentrations and the inter-day precision was less than 6% for all of 
      the analytes. The procedure was applied to a quantitation of oral fluid (OF) 
      samples that were collected during the Baltic Weekend Music Festival that was 
      held in Estonia.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Saar-Reismaa, Piret
AU  - Saar-Reismaa P
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia.
FAU - Tretjakova, Anastassia
AU  - Tretjakova A
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia.
FAU - Mazina-Sinkar, Jekaterina
AU  - Mazina-Sinkar J
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia.
FAU - Vaher, Merike
AU  - Vaher M
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia.
FAU - Kaljurand, Mihkel
AU  - Kaljurand M
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia.
FAU - Kulp, Maria
AU  - Kulp M
AD  - Tallinn University of Technology, Department of Chemistry and Biotechnology, 
      Akadeemia Tee 15, Tallinn, Estonia. Electronic address: maria.kulp@taltech.ee.
LA  - eng
PT  - Journal Article
DEP - 20190108
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Body Fluids/*chemistry
MH  - Electrophoresis, Capillary
MH  - Humans
MH  - Molecular Structure
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis
OTO - NOTNLM
OT  - Capillary electrophoresis
OT  - Ecstasy
OT  - Fluorescence
OT  - Oral fluid
EDAT- 2019/02/18 06:00
MHDA- 2019/04/09 06:00
CRDT- 2019/02/18 06:00
PHST- 2018/08/28 00:00 [received]
PHST- 2019/01/03 00:00 [revised]
PHST- 2019/01/07 00:00 [accepted]
PHST- 2019/02/18 06:00 [entrez]
PHST- 2019/02/18 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
AID - S0039-9140(19)30029-3 [pii]
AID - 10.1016/j.talanta.2019.01.029 [doi]
PST - ppublish
SO  - Talanta. 2019 May 15;197:390-396. doi: 10.1016/j.talanta.2019.01.029. Epub 2019 
      Jan 8.