PMID- 30771955
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20190408
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 197
DP  - 2019 May 15
TI  - Laser ablation ICP-MS for simultaneous quantitative imaging of iron and 
      ferroportin in hippocampus of human brain tissues with Alzheimer's disease.
PG  - 413-421
LID - S0039-9140(19)30056-6 [pii]
LID - 10.1016/j.talanta.2019.01.056 [doi]
AB  - Laser ablation inductively coupled plasma - mass spectrometry (LA-ICP-MS) is 
      proposed for a better understanding of metals and proteins distribution in 
      micrometre structures of human brain tissues. Simultaneous absolute quantitative 
      imaging of Fe and ferroportin (FPN), in 5 microm thick tissue sections of the stratum 
      pyramidale of hippocampus CA1 region, was carried out for Alzheimer disease (AD) 
      patients and healthy controls (HC). For the imaging of FPN by LA-ICP-MS, 
      antibodies were labelled via carbodiimide crosslinking with fluorescent gold 
      nanoclusters (AuNCs) of 2.2 nm diameter, enabling a high amplification (314 gold 
      atoms per NC). Laboratory made gelatin standards containing Fe and Au were used 
      for LA-ICP-MS calibration. Results showed that iron presents an increased 
      concentration in AD donors compared with HC donors, whereas similar 
      concentrations of FPN in AD donors with respect to HC donors were obtained. The 
      average absolute FPN concentrations in selected areas obtained with the proposed 
      AuNCs method were compared with the levels obtained by densitometric analysis 
      with a traditional IHC approach, observing a similar trend in all cases.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Cruz-Alonso, Maria
AU  - Cruz-Alonso M
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria 8, 33006 Oviedo, Spain.
FAU - Fernandez, Beatriz
AU  - Fernandez B
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria 8, 33006 Oviedo, Spain. Electronic address: 
      fernandezbeatriz@uniovi.es.
FAU - Navarro, Ana
AU  - Navarro A
AD  - Department of Morphology and Cell Biology, Faculty of Medicine, University of 
      Oviedo, Julian Claveria 6, 33006 Oviedo, Spain.
FAU - Junceda, Susana
AU  - Junceda S
AD  - Service of Anatomo-Pathology, Hospital Universitario Central de Asturias - HUCA, 
      Av Roma s/n, 33011 Oviedo, Spain.
FAU - Astudillo, Aurora
AU  - Astudillo A
AD  - Service of Anatomo-Pathology, Hospital Universitario Central de Asturias - HUCA, 
      Av Roma s/n, 33011 Oviedo, Spain.
FAU - Pereiro, Rosario
AU  - Pereiro R
AD  - Department of Physical and Analytical Chemistry, Faculty of Chemistry, University 
      of Oviedo, Julian Claveria 8, 33006 Oviedo, Spain.
LA  - eng
PT  - Journal Article
DEP - 20190115
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Cation Transport Proteins)
RN  - 0 (metal transporting protein 1)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Alzheimer Disease/*metabolism
MH  - Case-Control Studies
MH  - Cation Transport Proteins/*analysis
MH  - Hippocampus/*chemistry
MH  - Humans
MH  - Iron/*analysis
MH  - *Laser Therapy
MH  - Mass Spectrometry
MH  - *Optical Imaging
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Ferroportin
OT  - Gold nanoclusters
OT  - Iron accumulation
OT  - Laser ablation ICP-MS
OT  - Quantitative bioimaging
EDAT- 2019/02/18 06:00
MHDA- 2019/04/09 06:00
CRDT- 2019/02/18 06:00
PHST- 2018/12/03 00:00 [received]
PHST- 2019/01/11 00:00 [revised]
PHST- 2019/01/14 00:00 [accepted]
PHST- 2019/02/18 06:00 [entrez]
PHST- 2019/02/18 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
AID - S0039-9140(19)30056-6 [pii]
AID - 10.1016/j.talanta.2019.01.056 [doi]
PST - ppublish
SO  - Talanta. 2019 May 15;197:413-421. doi: 10.1016/j.talanta.2019.01.056. Epub 2019 
      Jan 15.