PMID- 30773095
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20201223
IS  - 1538-0254 (Electronic)
IS  - 0739-1102 (Linking)
VI  - 38
IP  - 2
DP  - 2020 Feb
TI  - Determining the interaction behavior of calf thymus DNA with berberine 
      hydrochloride in the presence of linker histone: a biophysical study.
PG  - 364-381
LID - 10.1080/07391102.2019.1574240 [doi]
AB  - The binding of small molecules with histone-DNA complexes can cause an 
      interference in vital cellular processes such as cell division and the growth of 
      cancerous cells that results in apoptosis. It is significant to study the 
      interaction of small molecules with histone-DNA complex for the purpose of better 
      understanding their mechanism of action, as well as designing novel and more 
      effective drug compounds. The fluorescence quenching of ct-DNA upon interaction 
      with Berberine has determined the binding of Berberine to ct-DNA with 
      K(sv) = 9.46 x 10(7) M(-1). K(sv) value of ct-DNA-Berberine in the presence of H1 
      has been observed to be 3.10 x 10(7) M(-1), indicating that the H1 has caused a 
      reduction in the binding affinity of Berberine to ct-DNA. In the competitive 
      emission spectrum, ethidium bromide (EB) and acridine orange (AO) have been 
      examined as intercalators through the addition of Berberine to ct-DNA complexes, 
      which includes ctDNA-EB and ctDNA-AO. Although in the presence of histone H1 , we 
      have observed signs of competition through the induced changes within the 
      emission spectra, yet there has been apparently no competition between the 
      ligands and probes. The viscosity results have confirmed the different behaviors 
      of interaction between ctDNA and Berberine throughout the binary and ternary 
      systems. We have figured out the IC50 and viability percent values at three 
      different time durations of interaction between Berberine and MCF7 cell line. The 
      molecular experiments have been completed by achieving the results of MTT assay, 
      which have been confirmed to be in good agreement with molecular modeling 
      studies.Communicated by Ramaswamy H. Sarma.
FAU - Roudini, Louisa
AU  - Roudini L
AD  - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, Mashhad, Iran.
FAU - NayebZadeh Eidgahi, Negar
AU  - NayebZadeh Eidgahi N
AD  - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, Mashhad, Iran.
FAU - Rahimi, Hamid Reza
AU  - Rahimi HR
AD  - Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
AD  - Department of Modern Sciences & Technologies, School of Medicine, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Saberi, Mohammad Reza
AU  - Saberi MR
AD  - Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Amiri Tehranizadeh, Zeinab
AU  - Amiri Tehranizadeh Z
AD  - Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Beigoli, Sima
AU  - Beigoli S
AD  - Endoscopic and Minimally Invasive Surgery Research Center, Mashhad University of 
      Medical Sciences, Mashhad, Iran.
FAU - Chamani, Jamshidkhan
AU  - Chamani J
AD  - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad 
      University, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190217
PL  - England
TA  - J Biomol Struct Dyn
JT  - Journal of biomolecular structure & dynamics
JID - 8404176
RN  - 0 (Histones)
RN  - 0I8Y3P32UF (Berberine)
RN  - 1C4QK22F9J (Potassium Iodide)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Animals
MH  - Berberine/chemistry/*metabolism/pharmacology
MH  - Binding, Competitive
MH  - *Biophysical Phenomena
MH  - Cattle
MH  - Cell Death/drug effects
MH  - DNA/chemistry/*metabolism
MH  - Ethidium/chemistry
MH  - Histones/chemistry/*metabolism
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Kinetics
MH  - MCF-7 Cells
MH  - Molecular Docking Simulation
MH  - Osmolar Concentration
MH  - Potassium Iodide/pharmacology
MH  - Protein Structure, Secondary
MH  - Scattering, Radiation
MH  - Sodium Chloride/pharmacology
MH  - Spectrometry, Fluorescence
MH  - Thermodynamics
MH  - Viscosity
OTO - NOTNLM
OT  - Berberine chloride
OT  - DNA interaction
OT  - MTT assay
OT  - cell culture
OT  - linker histone
OT  - molecular modeling
OT  - spectroscopy
EDAT- 2019/02/19 06:00
MHDA- 2020/12/29 06:00
CRDT- 2019/02/19 06:00
PHST- 2019/02/19 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2019/02/19 06:00 [entrez]
AID - 10.1080/07391102.2019.1574240 [doi]
PST - ppublish
SO  - J Biomol Struct Dyn. 2020 Feb;38(2):364-381. doi: 10.1080/07391102.2019.1574240. 
      Epub 2019 Feb 17.