PMID- 30775337
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220331
IS  - 2248-2997 (Print)
IS  - 2248-2997 (Electronic)
IS  - 2248-2997 (Linking)
VI  - 8
IP  - 4
DP  - 2018 Dec
TI  - High level aminoglycoside resistant enterococci in hospital-acquired urinary 
      tract infections in Mansoura, Egypt.
PG  - 186-190
LID - 10.18683/germs.2018.1145 [doi]
AB  - INTRODUCTION: Resistance to different antimicrobial agents is increasing in 
      enterococci and effective treatment represents a major health concern. The aim of 
      this study was to determine the antimicrobial resistance patterns and the 
      frequency of high level aminoglycoside resistance (HLAR) among enterococci. 
      METHODS: A total of 80 enterococcal isolates, (73 Enterococcus faecalis, 7 
      Enterococcus faecium) were collected from patients with hospital acquired urinary 
      tract infections (UTI) at Mansoura University hospitals in Egypt. Antimicrobial 
      susceptibility testing was performed via the disc diffusion method. PCR was used 
      for identification of species and detection of aminoglycoside-modifying enzymes 
      genes (AME). RESULTS: All enterococcal isolates were sensitive to vancomycin and 
      linezolid. Fifty-three isolates exhibited HLAR. Our results show that HLAR was 
      mediated by the presence of multiple AMEs genes. The aac(6')-Ie-aph(2')-Ia gene 
      was associated with aph(3')-IIIa and ant(6)-Ia gene in 69% of HLAR isolates. 
      CONCLUSION: This study showed that enterococci isolated from hospital acquired 
      UTI were resistant to multiple antibiotics. Furthermore, the frequency of high 
      level gentamicin resistance (HLGR) was higher than high level of streptomycin 
      resistance (HLSR). The most common AME genes were aph(3')-IIIa and ant(6)-Ia 
      followed by aac(6')-Ie-aph(2')-Ia.
FAU - El-Mahdy, Rasha
AU  - El-Mahdy R
AD  - MD, Department of Medical Microbiology and Immunology, Faculty of Medicine, 
      Mansoura University, Mansoura 35516, Egypt.
FAU - Mostafa, Ahmed
AU  - Mostafa A
AD  - MSc, Department of Medical Microbiology and Immunology, Faculty of Medicine, 
      Mansoura University, Mansoura 35516, Egypt.
FAU - El-Kannishy, Ghada
AU  - El-Kannishy G
AD  - MD, Department of Internal Medicine, Faculty of Medicine, Mansoura University, 
      Mansoura 35516, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20181203
PL  - Romania
TA  - Germs
JT  - Germs
JID - 101596099
PMC - PMC6363399
OTO - NOTNLM
OT  - High level aminoglycoside resistance
OT  - enterococci
OT  - urinary tract infection
COIS- Conflicts of interest: All authors - none to declare.
EDAT- 2019/02/19 06:00
MHDA- 2019/02/19 06:01
PMCR- 2018/12/03
CRDT- 2019/02/19 06:00
PHST- 2018/09/30 00:00 [received]
PHST- 2018/11/11 00:00 [revised]
PHST- 2018/11/14 00:00 [accepted]
PHST- 2019/02/19 06:00 [entrez]
PHST- 2019/02/19 06:00 [pubmed]
PHST- 2019/02/19 06:01 [medline]
PHST- 2018/12/03 00:00 [pmc-release]
AID - germs.2018.1145 [pii]
AID - 10.18683/germs.2018.1145 [doi]
PST - epublish
SO  - Germs. 2018 Dec 3;8(4):186-190. doi: 10.18683/germs.2018.1145. eCollection 2018 
      Dec.