PMID- 30775864
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2379-8068 (Electronic)
IS  - 2379-8068 (Linking)
VI  - 1
IP  - 2
DP  - 2016 Mar
TI  - Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones.
PG  - 359-371
LID - 10.1002/cppb.20022 [doi]
AB  - Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of 
      chromosome-specific DNA probes to identify large chromosome regions, chromosome 
      arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in 
      plants, most often, contigs of chromosome-specific bacterial artificial 
      chromosomes (BAC) from the species of origin or a related species are used as 
      painting probes. CP enables visualization and tracing of particular chromosome 
      regions and/or chromosomes throughout all mitotic and meiotic stages as well as 
      the corresponding interphase chromosome territories. CCP enables identification 
      of large-scale homeologous chromosome regions and chromosomes shared among two or 
      more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis 
      thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of 
      Arabidopsis chromosome-specific BAC contigs is described. (c) 2016 by John Wiley & 
      Sons, Inc.
CI  - Copyright (c) 2016 John Wiley & Sons, Inc.
FAU - Mandakova, Terezie
AU  - Mandakova T
AD  - Central European Institute of Technology (CEITEC), Masaryk University, Czech 
      Republic.
FAU - Lysak, Martin A
AU  - Lysak MA
AD  - Central European Institute of Technology (CEITEC), Masaryk University, Czech 
      Republic.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Plant Biol
JT  - Current protocols in plant biology
JID - 101685882
OTO - NOTNLM
OT  - Arabidopsis
OT  - BAC FISH
OT  - Brassicaceae
OT  - chromosome painting
OT  - fluorescence in situ hybridization (FISH)
OT  - nick translation
OT  - pachytene chromosomes
EDAT- 2016/03/01 00:00
MHDA- 2016/03/01 00:01
CRDT- 2019/02/19 06:00
PHST- 2019/02/19 06:00 [entrez]
PHST- 2016/03/01 00:00 [pubmed]
PHST- 2016/03/01 00:01 [medline]
AID - 10.1002/cppb.20022 [doi]
PST - ppublish
SO  - Curr Protoc Plant Biol. 2016 Mar;1(2):359-371. doi: 10.1002/cppb.20022.