PMID- 30779211
OWN - NLM
STAT- MEDLINE
DCOM- 20190502
LR  - 20200930
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 42
IP  - 4
DP  - 2019 Apr
TI  - Improvement of a diagnostic procedure in surveillance of the listed fish diseases 
      IHN and VHS.
PG  - 559-572
LID - 10.1111/jfd.12968 [doi]
AB  - Infectious haematopoietic necrosis (IHN) and viral haemorrhagic septicaemia (VHS) 
      are OIE-listed and notifiable viral fish diseases which are controlled by 
      eradication and surveillance programmes globally. The present study provides 
      improved RT-qPCR procedures based on recently described OIE protocols. 
      Improvements comprise the design of a new TaqMan(R) probe, replacing a TaqMan(R) MGB 
      probe that turned out to show impaired binding. Reason for this is SNPs detected 
      in the nucleoprotein N gene sequences of IHNV strains targeted by the RT-qPCR. 
      Furthermore, the IHNV and VHSV RT-qPCR assays were realized as one-step and 
      one-run procedures supplemented by an endogenous control system. The IHNV and 
      VHSV RT-qPCR assays are characterized by a technical sensitivity of 19 and 190 
      gene equivalents (cRNA) and an analytical sensitivity of 2-7 and 13 TCID(50) /ml, 
      respectively. For verification purposes, 105 IHNV and 165 VHSV isolates and 
      several non-targeted viral and bacterial pathogens were included and returned 
      adequate results. However, in field samples divergent results left 14 samples of 
      154 undetected for IHNV and one sample of 127 for VHSV using cell culture. The 
      study shows that RT-qPCR assays ensure facilitated and reliable testing on IHNV 
      and VHSV in eradication and surveillance programmes.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Hoferer, Marc
AU  - Hoferer M
AD  - Chemisches und Veterinaruntersuchungsamt Stuttgart (Chemical and Veterinary 
      Investigations Office Stuttgart), Fellbach, Germany.
FAU - Akimkin, Valerij
AU  - Akimkin V
AD  - Chemisches und Veterinaruntersuchungsamt Stuttgart (Chemical and Veterinary 
      Investigations Office Stuttgart), Fellbach, Germany.
FAU - Skrypski, Julia
AU  - Skrypski J
AD  - Chemisches und Veterinaruntersuchungsamt Stuttgart (Chemical and Veterinary 
      Investigations Office Stuttgart), Fellbach, Germany.
FAU - Schutze, Heike
AU  - Schutze H
AD  - Federal Research Institute for Animal Health, Institute of Infectology, Friedrich 
      Loeffler Institute, Greifswald-Insel Riems, Germany.
FAU - Sting, Reinhard
AU  - Sting R
AUID- ORCID: 0000-0002-1437-5036
AD  - Chemisches und Veterinaruntersuchungsamt Stuttgart (Chemical and Veterinary 
      Investigations Office Stuttgart), Fellbach, Germany.
LA  - eng
SI  - GENBANK/MK241784
SI  - GENBANK/MK234583
SI  - GENBANK/MK234584
SI  - GENBANK/MK234585
SI  - GENBANK/MK234586
SI  - GENBANK/AY331657
SI  - GENBANK/X89213
SI  - GENBANK/L40882
SI  - GENBANK/L40883
SI  - GENBANK/NC001652
SI  - GENBANK/L40880
SI  - GENBANK/U15170
SI  - GENBANK/L40881
SI  - GENBANK/X73873
SI  - GENBANK/AF343572
SI  - GENBANK/Y18263
SI  - GENBANK/KF783182
PT  - Journal Article
DEP - 20190219
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Nucleoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Epidemiological Monitoring/*veterinary
MH  - Fish Diseases/*diagnosis/epidemiology/virology
MH  - Fishes/virology
MH  - Hemorrhagic Septicemia, Viral/*diagnosis/epidemiology
MH  - Infectious hematopoietic necrosis virus/genetics
MH  - Novirhabdovirus/genetics
MH  - Nucleoproteins/genetics
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Rhabdoviridae Infections/diagnosis/epidemiology/*veterinary
MH  - Sensitivity and Specificity
MH  - Viral Proteins/genetics
OTO - NOTNLM
OT  - RT-qPCR
OT  - diagnostic validation
OT  - endogenous control system
OT  - field validation
OT  - infectious haematopoietic necrosis virus
OT  - viral haemorrhagic septicaemia virus
EDAT- 2019/02/20 06:00
MHDA- 2019/05/03 06:00
CRDT- 2019/02/20 06:00
PHST- 2018/08/24 00:00 [received]
PHST- 2018/12/19 00:00 [revised]
PHST- 2018/12/20 00:00 [accepted]
PHST- 2019/02/20 06:00 [pubmed]
PHST- 2019/05/03 06:00 [medline]
PHST- 2019/02/20 06:00 [entrez]
AID - 10.1111/jfd.12968 [doi]
PST - ppublish
SO  - J Fish Dis. 2019 Apr;42(4):559-572. doi: 10.1111/jfd.12968. Epub 2019 Feb 19.