PMID- 30780011
OWN - NLM
STAT- MEDLINE
DCOM- 20190318
LR  - 20190318
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1110-1111
DP  - 2019 Mar 15
TI  - Determination of the total tulathromycin residues in bovine muscle, fat, and 
      liver by liquid chromatography-tandem mass spectrometry.
PG  - 51-58
LID - S1570-0232(18)31672-6 [pii]
LID - 10.1016/j.jchromb.2019.02.011 [doi]
AB  - A reliable and accurate liquid chromatography-tandem mass spectrometry 
      (LC-MS/MS)-based method was developed to quantify total tulathromycin residues in 
      bovine tissues. Specifically, the above method relied on the quantification of 
      CP-60,300, a marker produced by tulathromycin hydrolysis, for which maximum 
      residue limits (MRLs) were established by the European Union and several other 
      countries. Sample preparation and LC-MS/MS conditions were thoroughly optimized 
      to allow for accurate quantification. The optimized procedure involved sample 
      homogenization with 2 mol/L hydrochloric acid and ethyl acetate, heating of the 
      resulting aqueous layer to convert tulathromycin and its metabolites into the 
      marker residue, cleanup by a polymer-based cation-exchange cartridge, and 
      subsequent analysis by LC-MS/MS. The developed method was validated for 
      tulathromycin A and the marker residue in bovine muscle, fat, and liver at two 
      levels, namely at the MRL set in Japan and at 0.01 mg/kg. Excellent analytical 
      performance was observed, with the average recoveries of tulathromycin A and the 
      marker residue ranging from 98 to 107%, and relative standard deviations ranging 
      from 1 to 3%. Matrix effects were negligible, and analyte loss during sample 
      preparation was minimal for all matrices tested, which allowed for accurate 
      determination by external standard calibration using a solvent standard. No 
      interfering peaks were observed close to the retention time of the marker residue 
      for all matrices, which was indicative of high specificity. Overall, the 
      developed method was proven suitable for regulatory purpose analysis of total 
      tulathromycin residues.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Saito-Shida, Shizuka
AU  - Saito-Shida S
AD  - Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, 
      Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: 
      shizsaito@nihs.go.jp.
FAU - Kashiwabara, Nao
AU  - Kashiwabara N
AD  - Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, 
      Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
FAU - Nemoto, Satoru
AU  - Nemoto S
AD  - Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, 
      Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
FAU - Akiyama, Hiroshi
AU  - Akiyama H
AD  - Division of Foods, National Institute of Health Sciences, Tonomachi 3-25-26, 
      Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
LA  - eng
PT  - Journal Article
DEP - 20190212
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Disaccharides)
RN  - 0 (Heterocyclic Compounds)
RN  - Q839I13422 (tulathromycin)
SB  - IM
MH  - Adipose Tissue/chemistry
MH  - Animals
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Disaccharides/*analysis/pharmacokinetics
MH  - Drug Residues/*analysis/pharmacokinetics
MH  - Heterocyclic Compounds/*analysis/pharmacokinetics
MH  - Limit of Detection
MH  - Linear Models
MH  - Liver/*chemistry
MH  - Muscle, Skeletal/*chemistry
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
MH  - Tissue Distribution
OTO - NOTNLM
OT  - Bovine tissue
OT  - Hydrolysis
OT  - LC-MS/MS
OT  - Tulathromycin
EDAT- 2019/02/20 06:00
MHDA- 2019/03/19 06:00
CRDT- 2019/02/20 06:00
PHST- 2018/11/06 00:00 [received]
PHST- 2019/01/19 00:00 [revised]
PHST- 2019/02/10 00:00 [accepted]
PHST- 2019/02/20 06:00 [pubmed]
PHST- 2019/03/19 06:00 [medline]
PHST- 2019/02/20 06:00 [entrez]
AID - S1570-0232(18)31672-6 [pii]
AID - 10.1016/j.jchromb.2019.02.011 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Mar 15;1110-1111:51-58. doi: 
      10.1016/j.jchromb.2019.02.011. Epub 2019 Feb 12.