PMID- 30781012
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 91
IP  - 2
DP  - 2007 Feb
TI  - A New Potyvirus found in Passiflora incence in Florida.
PG  - 227
LID - 10.1094/PDIS-91-2-0227A [doi]
AB  - During March of 2004 (Alachua County) and again during February of 2006 
      (Highlands County), specimens of the plant Passiflora incence (passionfruit) with 
      chlorotic symptoms were submitted to the Division of Plant Industry, Gainesville, 
      FL for diagnosis. Cytoplasmic cylindrical inclusions seen in epidermal strips of 
      plant leaves stained in Luxol brilliant green and calcomine orange but not seen 
      in those stained in Azure A indicated the presence of a potyvirus infection. Leaf 
      dips made for electron microscopy also showed virus particles consistent with a 
      potyvirus infection. Reverse-transcription (RT)-PCR using degenerate potyvirus 
      primers (4) produced a target  approximately 1.7-kb potyvirus band. Approximately 1.4 kb of the 
      PCR fragments from both specimens was sequenced and was 100% identical. The 
      1.4-kb fragment contained the 3' end of the NIb region, the coat protein, and the 
      beginning of the 3'UTR. A GenBank BLAST search found that the two most similar 
      potyviruses were Bean common mosaic necrosis virus (BCMNV), also known as 
      serotype A of Bean common mosaic virus (BCMV), and Soybean mosaic virus (SMV). 
      Molecular analysis of the 1.4-kb sequence using MegAlign (DNASTAR, Madison, WI) 
      indicated a 73% identity with BCMNV and 68.8% with SMV. Analysis of the coat 
      protein showed the highest identity (87%) with BCMNV, and for the NIb region, the 
      highest identity occurred with SMV at 78%. In double-antibody sandwich 
      (DAS)-ELISA, the virus did not react with antisera to either BCMV or BCMNV 
      (BioReba). In sodium dodecyl sulfate (SDS)-immunodiffusion tests, however, the 
      virus reacted heterologously with antiserum to Peanut stripe virus, now 
      considered a member of the serotype B group of BCMV (1). In host range studies, 
      this virus induced systemic chlorosis in Chenopodium quinoa but did not cause 
      symptoms on any other host inoculated, including eight leguminous species and 
      Nicotiana benthamiana. N. bethamiana is susceptible to BCMV and three other known 
      Passiflora potyviruses, Passionfruit crinkle virus (PCV), Passionfruit woodiness 
      virus (PWV), and Passionfruit mottle virus (PFMoV) (2,3). The cylindrical 
      inclusions of this virus seen with the light microscope appeared as loosely 
      aggregated medium length plate or needle-like structures as opposed to long, 
      compact, bundle-shaped aggregates (PFMoV and PCV) or short plate-like structures 
      (PWV) (2,3). The virus did not react with antisera (W. Zettler, University of 
      Florida) to the three aforementioned passionfruit potyviruses in 
      SDS-immunodiffusion tests. This virus, like PCV, PWV, and PFMoV, is related to 
      the Bean common mosaic virus group. However, based on cylindrical inclusion 
      morphology, host range, serology, and genetic sequencing, the virus appears to be 
      a new potyvirus infecting Passiflora and is tentatively named Passiflora 
      chlorosis virus. The sequence was deposited in GenBank as Accession No. DQ860147. 
      References: (1) A. A Brunt et al. Plant Viruses Online: Descriptions and Lists 
      from the VIDE Database. Version 20, August 1996. (2) C. A. Chang. Phytopathology. 
      82:1358, 1992 (3) C. A. Chang et al. Plant Prot. Bull. 38:339, 1996. (4) A. Gibbs 
      and A. Mackenzie. J. Virol. Methods 63:9, 1997.
FAU - Baker, C A
AU  - Baker CA
AD  - Florida Department of Agriculture and Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
FAU - Jones, L
AU  - Jones L
AD  - Florida Department of Agriculture and Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
EDAT- 2007/02/01 00:00
MHDA- 2007/02/01 00:01
CRDT- 2019/02/21 06:00
PHST- 2019/02/21 06:00 [entrez]
PHST- 2007/02/01 00:00 [pubmed]
PHST- 2007/02/01 00:01 [medline]
AID - 10.1094/PDIS-91-2-0227A [doi]
PST - ppublish
SO  - Plant Dis. 2007 Feb;91(2):227. doi: 10.1094/PDIS-91-2-0227A.