PMID- 30781538
OWN - NLM
STAT- MEDLINE
DCOM- 20190529
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 4
DP  - 2019 Feb 16
TI  - Circadian Expression of TIMP3 Is Disrupted by UVB Irradiation and Recovered by 
      Green Tea Extracts.
LID - 10.3390/ijms20040862 [doi]
LID - 862
AB  - The human skin is the outermost physical barrier and has its own circadian 
      machinery that works either cooperatively with the central clock, or 
      autonomously. Circadian rhythms have been observed in many functions related to 
      epidermal homeostasis including hydration and inflammation, and this functional 
      oscillation is disturbed by ultraviolet radiation (UVR), which is a strong 
      environmental cue. Among the genes estimated to show circadian expression in the 
      skin, metalloproteinase inhibitor 3 (TIMP3), has a rhythmic expression in 
      synchronized human keratinocytes similar to that of the core clock gene PER1 and 
      an epidermal circadian regulatory gene, aquaporin 3 (AQP3) but was antiphase to 
      the core clock gene BMAL1. Tumor necrosis factor-alpha (TNF-alpha), the regulatory target 
      of TIMP3 via a disintegrin and metalloproteinase domain 17 (ADAM17), was 
      inversely regulated when TIMP3 expression was downregulated by ultraviolet B 
      (UVB) treatment. When synthetic TIMP3 peptides were applied to the cells, the 
      secretion of TNF-alpha did not increase following the UVB treatment. Similar to TIMP3 
      peptides, Camellia sinensis leaf-derived extracts showed a distinguishing 
      efficacy in recovering TIMP3 expression, downregulated by UVB treatment. 
      Together, our results suggest that TIMP3 reversely mediates UVR-induced 
      inflammation by being highly expressed during the daytime; therefore, recovering 
      the circadian expression of TIMP3 using synthetic TIMP3 peptides or bioactive 
      natural ingredients could at least in part inhibit the UVR-induced cellular 
      phenomena.
FAU - Park, Sunyoung
AU  - Park S
AD  - Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 
      Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, Korea. 
      sypark@amorepacific.com.
FAU - Lee, Eun-Soo
AU  - Lee ES
AUID- ORCID: 0000-0002-1600-3615
AD  - Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 
      Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, Korea. 
      soopian@amorepacific.com.
FAU - Park, Nok-Hyun
AU  - Park NH
AD  - Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 
      Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, Korea. 
      aquareve@amorepacific.com.
FAU - Hwang, Kyeonghwan
AU  - Hwang K
AD  - Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 
      Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, Korea. 
      khhwang@amorepacific.com.
FAU - Cho, Eun-Gyung
AU  - Cho EG
AUID- ORCID: 0000-0002-3615-5763
AD  - Basic Research & Innovation Division, R&D Unit, AmorePacific Corporation, 1920 
      Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, Korea. 
      egcho@amorepacific.com.
LA  - eng
PT  - Journal Article
DEP - 20190216
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (AQP3 protein, human)
RN  - 0 (ARNTL Transcription Factors)
RN  - 0 (PER1 protein, human)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Plant Extracts)
RN  - 0 (TIMP3 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 158801-98-0 (Aquaporin 3)
RN  - EC 3.4.24.86 (ADAM17 Protein)
SB  - IM
MH  - ADAM17 Protein/genetics
MH  - ARNTL Transcription Factors/genetics
MH  - Aquaporin 3/genetics
MH  - Camellia sinensis/*chemistry
MH  - Cell Line
MH  - Cell Survival/drug effects/radiation effects
MH  - Circadian Rhythm/drug effects/genetics/radiation effects
MH  - Gene Expression Regulation, Developmental/drug effects/radiation effects
MH  - Humans
MH  - Inflammation/*drug therapy/genetics/pathology
MH  - Period Circadian Proteins/genetics
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Tissue Inhibitor of Metalloproteinase-3/*genetics
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Ultraviolet Rays
PMC - PMC6412890
OTO - NOTNLM
OT  - AQP3
OT  - Camellia sinensis leaves
OT  - TIMP3
OT  - UVB
OT  - anti-inflammation
OT  - circadian rhythm
COIS- All authors are employees of AmorePacific Corporation.
EDAT- 2019/02/20 06:00
MHDA- 2019/05/30 06:00
PMCR- 2019/02/01
CRDT- 2019/02/21 06:00
PHST- 2018/12/19 00:00 [received]
PHST- 2019/01/29 00:00 [revised]
PHST- 2019/02/12 00:00 [accepted]
PHST- 2019/02/21 06:00 [entrez]
PHST- 2019/02/20 06:00 [pubmed]
PHST- 2019/05/30 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - ijms20040862 [pii]
AID - ijms-20-00862 [pii]
AID - 10.3390/ijms20040862 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Feb 16;20(4):862. doi: 10.3390/ijms20040862.