PMID- 30781605 OWN - NLM STAT- MEDLINE DCOM- 20190531 LR - 20231006 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 20 IP - 4 DP - 2019 Feb 18 TI - Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. LID - 10.3390/ijms20040873 [doi] LID - 873 AB - This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 muL/nostril, 100 mg/mL, 10% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 x 10(6) MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-alpha; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-beta (TGF-beta) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination. FAU - Ebrahim, Nesrine AU - Ebrahim N AD - Department of Histology and Cell Biology, Benha University, Benha, Qalyubia 13518, Egypt. nesrien.salem@fmed.bu.edu.eg. AD - Stem Cell Unit, Benha University, Benha, Qalyubia 13518, Egypt. nesrien.salem@fmed.bu.edu.eg. FAU - Mandour, Yasser Mohammad Hassan AU - Mandour YMH AD - Department of Otorhinolaryngology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. yasser.mandour@fmed.bu.edu.eg. FAU - Farid, Ayman Samir AU - Farid AS AUID- ORCID: 0000-0002-7844-7967 AD - Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalyubia 13736, Egypt. ayman.samir@fvtm.bu.edu.eg. FAU - Nafie, Ebtesam AU - Nafie E AD - Zoology Department, Faculty of Science, Benha University, Benha 13518, Egypt. ebtesam.nafe@fsc.bu.edu.eg. FAU - Mohamed, Amira Zaky AU - Mohamed AZ AD - Department of Microbiology, Faculty of Science, Tanta University, Tanta 31527, Egypt. a.shenawy.32016@alexu.edu.eg. FAU - Safwat, Miriam AU - Safwat M AD - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. Miriam.safwat@kaseralainy.edu.eg. FAU - Taha, Radwa AU - Taha R AD - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. radwa.taha@kaseralainy.edu.eg. FAU - Sabry, Dina AU - Sabry D AUID- ORCID: 0000-0002-6720-3385 AD - Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. dinasabry@kasralainy.edu.eg. AD - Molecular Biology and Stem Cell Unit, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. dinasabry@kasralainy.edu.eg. FAU - Sorour, Safwa M AU - Sorour SM AD - Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. safwa.sorour@fmed.bu.edu.eg. FAU - Refae, Ahmed AU - Refae A AD - Department of Otorhinolaryngology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt. ahmed.alrefaai@fmed.bu.edu.eg. LA - eng PT - Journal Article DEP - 20190218 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Chemokine CCL11) RN - 0 (Transforming Growth Factor beta) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Vascular Cell Adhesion Molecule-1) RN - 207137-56-2 (Interleukin-4) SB - IM MH - Adipose Tissue/*cytology MH - Animals MH - Cells, Cultured MH - Chemokine CCL11/genetics/metabolism MH - Disease Models, Animal MH - Interleukin-4/genetics/metabolism MH - Male MH - *Mesenchymal Stem Cell Transplantation MH - Mesenchymal Stem Cells/*cytology MH - Nasal Mucosa/pathology/ultrastructure MH - Rats MH - Rhinitis, Allergic/blood/genetics/*immunology/*therapy MH - Transforming Growth Factor beta/genetics/metabolism MH - Tumor Necrosis Factor-alpha/genetics/metabolism MH - Vascular Cell Adhesion Molecule-1/genetics/metabolism PMC - PMC6412869 OTO - NOTNLM OT - CCL11 OT - Montelukast OT - VCAM-1 OT - allergic rhinitis OT - antileukotriene OT - immunoglobulins OT - mesenchymal stem cells COIS- The authors declare no conflicts of interest. EDAT- 2019/02/20 06:00 MHDA- 2019/06/01 06:00 PMCR- 2019/02/01 CRDT- 2019/02/21 06:00 PHST- 2019/01/30 00:00 [received] PHST- 2019/02/13 00:00 [revised] PHST- 2019/02/14 00:00 [accepted] PHST- 2019/02/21 06:00 [entrez] PHST- 2019/02/20 06:00 [pubmed] PHST- 2019/06/01 06:00 [medline] PHST- 2019/02/01 00:00 [pmc-release] AID - ijms20040873 [pii] AID - ijms-20-00873 [pii] AID - 10.3390/ijms20040873 [doi] PST - epublish SO - Int J Mol Sci. 2019 Feb 18;20(4):873. doi: 10.3390/ijms20040873.