PMID- 30782247
OWN - NLM
STAT- MEDLINE
DCOM- 20190329
LR  - 20190329
IS  - 0031-7144 (Print)
IS  - 0031-7144 (Linking)
VI  - 74
IP  - 1
DP  - 2019 Jan 1
TI  - Species-specificity in the interaction of IgE with FcepsilonRI between humans and rats.
PG  - 29-33
LID - 10.1691/ph.2019.8771 [doi]
AB  - The interaction of immunoglobulin E (IgE) to its high-affinity receptor (FcepsilonRI) 
      plays a key role in triggering allergic reactions. However, it is still 
      controversial whether species specificity influences the binding ability between 
      humans and rodents. Recombinant hFcepsilonRIalpha / RBL-2H3 and hFcepsilonRIalpha / MGC-803 cells 
      were prepared and sensitized with hIgE/anti-hIgE antibody or DNP-IgE/DNP-BSA, 
      respectively. Species-specificity was investigated using immuno-fluorescent 
      analysis, beta-hexosaminidase release rate assay, intracellular calcium 
      concentration assay and apoptosis assay, and the results showed that there is 
      species-specificity in IgE/FcepsilonRI in humans and rats, and no cross-recognition 
      between IgE and FcepsilonRI in two species. These results should provide the 
      experimental evidence for further research on the pathogenesis and the drug 
      development of allergic diseases.
FAU - Li, Fei
AU  - Li F
FAU - Wang, Nannan
AU  - Wang N
FAU - Yang, Yanlei
AU  - Yang Y
FAU - Zhang, Su
AU  - Zhang S
FAU - Cui, Zhe
AU  - Cui Z
FAU - Zhang, Yanfen
AU  - Zhang Y
FAU - Liu, Zhongcheng
AU  - Liu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pharmazie
JT  - Die Pharmazie
JID - 9800766
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - Calcium/metabolism
MH  - Cell Line, Tumor
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/*immunology
MH  - Rats
MH  - Receptors, IgE/*immunology
MH  - Species Specificity
EDAT- 2019/02/21 06:00
MHDA- 2019/03/30 06:00
CRDT- 2019/02/21 06:00
PHST- 2019/02/21 06:00 [entrez]
PHST- 2019/02/21 06:00 [pubmed]
PHST- 2019/03/30 06:00 [medline]
AID - 10.1691/ph.2019.8771 [doi]
PST - ppublish
SO  - Pharmazie. 2019 Jan 1;74(1):29-33. doi: 10.1691/ph.2019.8771.