PMID- 30784348
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200930
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 18
IP  - 6-7
DP  - 2019 Mar-Apr
TI  - NF90 regulation of immune factor expression in response to malaria antigens.
PG  - 708-722
LID - 10.1080/15384101.2019.1580496 [doi]
AB  - Nuclear factor 90 (NF90) is a dual DNA- and RNA-binding protein expressed 
      ubiquitously in mammalian cells, including monocytes. Here, to elucidate the 
      function of NF90 in the immune response, we analyzed systematically its influence 
      on gene expression programs in the human monocytic cell line THP-1 expressing 
      normal or reduced NF90 levels. RNA sequencing analysis revealed many mRNAs 
      showing differential abundance in NF90-silenced cells, many of them encoding 
      proteins implicated in the response to immune stimuli and malaria infection. The 
      transcription of some of them (e.g. TNF, LILRB1, and CCL2 mRNAs) was modulated by 
      silencing NF90. Ribonucleoprotein immunoprecipitation (RIP) analysis further 
      revealed that a subset of these mRNAs associated directly with NF90. To 
      understand how NF90 influenced globally the immune response to malaria infection, 
      lysates of red blood cells infected with Plasmodium falciparum (iRBC lysates) or 
      uninfected/mock-infected (uRBC lysates) were used to treat THP-1 cells as a 
      surrogate of malaria infection. NF90 affected the stability of a few target 
      mRNAs, but influenced more generally the translation and secretion of the encoded 
      cytokines after treatment with either uRBC or iRBC lysates. Taken together, these 
      results indicate that NF90 contributes to repressing the immune response in cells 
      responding to P. falciparum infection and suggest that NF90 can be a therapeutic 
      target in malaria.
FAU - Idda, M Laura
AU  - Idda ML
AD  - a Laboratory of Genetics and Genomics , National Institute on Aging Intramural 
      Research Program, National Institutes of Health , Baltimore , MD , USA.
AD  - b Istituto di Ricerca Genetica e Biomedica , Consiglio Nazionale delle Ricerche 
      (CNR) , Cagliari , Italy.
FAU - Lodde, Valeria
AU  - Lodde V
AD  - b Istituto di Ricerca Genetica e Biomedica , Consiglio Nazionale delle Ricerche 
      (CNR) , Cagliari , Italy.
AD  - c Department of Biomedical Sciences , University of Sassari , Sassari , Italy.
FAU - Galleri, Grazia
AU  - Galleri G
AUID- ORCID: 0000-0001-5379-9387
AD  - c Department of Biomedical Sciences , University of Sassari , Sassari , Italy.
FAU - Martindale, Jennifer L
AU  - Martindale JL
AD  - a Laboratory of Genetics and Genomics , National Institute on Aging Intramural 
      Research Program, National Institutes of Health , Baltimore , MD , USA.
FAU - Munk, Rachel
AU  - Munk R
AD  - a Laboratory of Genetics and Genomics , National Institute on Aging Intramural 
      Research Program, National Institutes of Health , Baltimore , MD , USA.
FAU - Abdelmohsen, Kotb
AU  - Abdelmohsen K
AD  - a Laboratory of Genetics and Genomics , National Institute on Aging Intramural 
      Research Program, National Institutes of Health , Baltimore , MD , USA.
FAU - Cucca, Francesco
AU  - Cucca F
AD  - b Istituto di Ricerca Genetica e Biomedica , Consiglio Nazionale delle Ricerche 
      (CNR) , Cagliari , Italy.
AD  - c Department of Biomedical Sciences , University of Sassari , Sassari , Italy.
FAU - Gorospe, Myriam
AU  - Gorospe M
AD  - a Laboratory of Genetics and Genomics , National Institute on Aging Intramural 
      Research Program, National Institutes of Health , Baltimore , MD , USA.
LA  - eng
GR  - Z01 AG000511/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190308
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Cytokines)
RN  - 0 (ILF3 protein, human)
RN  - 0 (Nuclear Factor 90 Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cytokines/immunology
MH  - Humans
MH  - Immunoprecipitation/methods
MH  - Malaria/*immunology
MH  - Nuclear Factor 90 Proteins/*immunology
MH  - Plasmodium falciparum/immunology
MH  - RNA, Messenger/immunology
MH  - RNA-Binding Proteins/immunology
MH  - THP-1 Cells
MH  - Transcription, Genetic/immunology
PMC - PMC6464584
OTO - NOTNLM
OT  - NF90
OT  - RNA-binding protein
OT  - malaria
OT  - monocytes
OT  - ribonucleoprotein complex
OT  - translational control
EDAT- 2019/02/21 06:00
MHDA- 2020/04/14 06:00
PMCR- 2020/03/08
CRDT- 2019/02/21 06:00
PHST- 2020/03/08 00:00 [pmc-release]
PHST- 2019/02/21 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2019/02/21 06:00 [entrez]
AID - 1580496 [pii]
AID - 10.1080/15384101.2019.1580496 [doi]
PST - ppublish
SO  - Cell Cycle. 2019 Mar-Apr;18(6-7):708-722. doi: 10.1080/15384101.2019.1580496. 
      Epub 2019 Mar 8.