PMID- 30785129
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20190909
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 7
IP  - 5
DP  - 2019 Apr 23
TI  - Cationic polymer-derived carbon dots for enhanced gene delivery and cell imaging.
PG  - 1940-1948
LID - 10.1039/c8bm01578c [doi]
AB  - Carbon dots have attracted rapidly growing interest in recent years. In this 
      report, we prepared two cationic polymer-derived carbon dots (Taea-CD and 
      Cyclen-CD, collectively called C-dots) via a hydrothermal method. Transmission 
      electron microscopy (TEM) results show that the C-dots were sphere-like and the 
      size distribution was 1.8 +/- 0.4 nm for Taea-CD and 5.4 +/- 2 nm for Cyclen-CD. The 
      C-dots emitted bright blue fluorescence under UV light (365 nm). Confocal laser 
      scanning microscopy (CLSM) assay indicates that the C-dots-mediated transfection 
      process could be detected in real time, and their tunable fluorescence emission 
      under different wavelengths could satisfy varying requirements. Luciferase assay 
      indicates that the transformation from the polymer to CD is an effective strategy 
      to improve the transfection efficiency (TE) of the materials. Moreover, the 
      C-dots also exhibit higher serum tolerance and cell viability than commercially 
      available polyethyleneimine (PEI). These results demonstrate that the preparation 
      of carbon dots from polymers is a promising method for developing multifunctional 
      gene vectors with high TE and biocompatibility.
FAU - He, Xi
AU  - He X
AD  - Key Laboratory of Green Chemistry and Technology (Ministry of Education), College 
      of Chemistry, Sichuan University, Chengdu 610064, P. R. China. xqyu@scu.edu.cn 
      jzhang@scu.edu.cn.
FAU - Chen, Ping
AU  - Chen P
FAU - Zhang, Ji
AU  - Zhang J
FAU - Luo, Tian-Ying
AU  - Luo TY
FAU - Wang, Hai-Jiao
AU  - Wang HJ
FAU - Liu, Yan-Hong
AU  - Liu YH
FAU - Yu, Xiao-Qi
AU  - Yu XQ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Drug Carriers)
RN  - 7440-44-0 (Carbon)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Carbon/*chemistry
MH  - Drug Carriers/*chemistry
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - *Molecular Imaging
MH  - Nanoparticles/*chemistry
MH  - Particle Size
MH  - Plasmids/chemistry/genetics
MH  - Polyethyleneimine/*chemistry
MH  - *Transfection
EDAT- 2019/02/21 06:00
MHDA- 2019/09/10 06:00
CRDT- 2019/02/21 06:00
PHST- 2019/02/21 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2019/02/21 06:00 [entrez]
AID - 10.1039/c8bm01578c [doi]
PST - ppublish
SO  - Biomater Sci. 2019 Apr 23;7(5):1940-1948. doi: 10.1039/c8bm01578c.