PMID- 30785478
OWN - NLM
STAT- MEDLINE
DCOM- 20190313
LR  - 20200225
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 52
IP  - 2
DP  - 2019 Feb 14
TI  - Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes 
      cell autophagy, but has no influence on cell apoptosis.
PG  - e7843
LID - S0100-879X2019000200610 [pii]
LID - 10.1590/1414-431X20187843 [doi]
LID - e7843
AB  - Gastric cancer remains a serious threat to human health worldwide. Kaempferol is 
      a plant-derived flavonoid compound with a wide range of pharmacological 
      activities. This study aimed to investigate the effects of kaempferol on gastric 
      cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as 
      underlying potential mechanisms. Viability, proliferation, and apoptosis of 
      SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 
      assay, 5-btomo-2'-deoxyuridine incorporation assay, and annexin V-FITC/PI 
      staining, respectively. Quantitative reverse transcription PCR was performed to 
      measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 
      cells. Cell transfection was used to down-regulate the expression of miR-181a. 
      The protein expression levels of cyclin D1, bcl-2, bax, caspase 3, caspase 9, 
      autophagy-related gene 7, microtubule-associated protein 1 light chain 3-I 
      (LC3-I), LC3-II, Beclin 1, p62, mitogen-activated protein kinase (MAPK), 
      extracellular regulated protein kinases (ERK), and phosphatidylinositol 3 kinase 
      (PI3K) in SNU-216 cells were detected using western blotting. Results showed that 
      kaempferol significantly suppressed SNU-216 cell viability and proliferation but 
      had no influence on cell apoptosis. Further results suggested that kaempferol 
      significantly induced SNU-216 cell autophagy. The expression of miR-181a in 
      SNU-216 cells after kaempferol treatment was enhanced. Kaempferol significantly 
      inactivated MAPK/ERK and PI3K pathways in SNU-216 cells. Suppression of miR-181a 
      significantly reversed the kaempferol-induced MAPK/ERK and PI3K pathways 
      inactivation in SNU-216 cells. This research demonstrated that kaempferol 
      suppressed proliferation and promoted autophagy of human gastric cancer SNU-216 
      cells by up-regulating miR-181a and inactivating MAPK/ERK and PI3K pathways.
FAU - Zhang, Fan
AU  - Zhang F
AUID- ORCID: 0000-0002-1084-1183
AD  - Teaching and Research Department of Diagnostics, Jining Medical University, 
      Jining, China.
FAU - Ma, Cuimei
AU  - Ma C
AUID- ORCID: 0000-0002-8817-0789
AD  - Department of Gastroenterology, Affiliated Hospital of Jining Medical University, 
      Jining, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20190214
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Kaempferols)
RN  - 731P2LE49E (kaempferol)
SB  - IM
RIN - Braz J Med Biol Res. 2020;53(9):e7843retraction. PMID: 32756813
MH  - Apoptosis/*drug effects
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Humans
MH  - Kaempferols/*pharmacology
MH  - Stomach Neoplasms/*pathology
PMC - PMC6376319
EDAT- 2019/02/21 06:00
MHDA- 2019/03/14 06:00
PMCR- 2019/02/14
CRDT- 2019/02/21 06:00
PHST- 2018/07/22 00:00 [received]
PHST- 2018/11/19 00:00 [accepted]
PHST- 2019/02/21 06:00 [entrez]
PHST- 2019/02/21 06:00 [pubmed]
PHST- 2019/03/14 06:00 [medline]
PHST- 2019/02/14 00:00 [pmc-release]
AID - S0100-879X2019000200610 [pii]
AID - 10.1590/1414-431X20187843 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2019 Feb 14;52(2):e7843. doi: 10.1590/1414-431X20187843.