PMID- 30788834
OWN - NLM
STAT- MEDLINE
DCOM- 20190711
LR  - 20190711
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1940
DP  - 2019
TI  - Mouse Lung Tissue Slice Culture.
PG  - 297-311
LID - 10.1007/978-1-4939-9086-3_21 [doi]
AB  - Precision-cut lung slices (PCLS) represent an ex vivo model widely used in 
      visualizing interactions between lung structure and function. The major advantage 
      of this technique is that the presence, differentiation state, and localization 
      of the more than 40 cell types that make up the lung are in accordance with the 
      physiological situation found in lung tissue, including the right localization 
      and patterning of extracellular matrix elements. Here we describe the methodology 
      involved in preparing and culturing PCLS followed by detailed practical 
      information about their possible applications.
FAU - Wu, Xinhui
AU  - Wu X
AD  - Faculty of Science and Engineering, Department of Molecular Pharmacology, 
      University of Groningen, Groningen, The Netherlands.
AD  - Groningen Research Institute for Asthma and COPD, University Medical Center 
      Groningen, University of Groningen, Groningen, The Netherlands.
FAU - van Dijk, Eline M
AU  - van Dijk EM
AD  - Faculty of Science and Engineering, Department of Molecular Pharmacology, 
      University of Groningen, Groningen, The Netherlands.
AD  - Groningen Research Institute for Asthma and COPD, University Medical Center 
      Groningen, University of Groningen, Groningen, The Netherlands.
FAU - Bos, I Sophie T
AU  - Bos IST
AD  - Faculty of Science and Engineering, Department of Molecular Pharmacology, 
      University of Groningen, Groningen, The Netherlands.
AD  - Groningen Research Institute for Asthma and COPD, University Medical Center 
      Groningen, University of Groningen, Groningen, The Netherlands.
FAU - Kistemaker, Loes E M
AU  - Kistemaker LEM
AD  - Faculty of Science and Engineering, Department of Molecular Pharmacology, 
      University of Groningen, Groningen, The Netherlands.
AD  - Groningen Research Institute for Asthma and COPD, University Medical Center 
      Groningen, University of Groningen, Groningen, The Netherlands.
FAU - Gosens, Reinoud
AU  - Gosens R
AD  - Faculty of Science and Engineering, Department of Molecular Pharmacology, 
      University of Groningen, Groningen, The Netherlands. r.gosens@rug.nl.
AD  - Groningen Research Institute for Asthma and COPD, University Medical Center 
      Groningen, University of Groningen, Groningen, The Netherlands. r.gosens@rug.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - Extracellular Matrix/physiology
MH  - Lung/*cytology
MH  - Mice
MH  - Organ Culture Techniques/*methods
MH  - Tissue Culture Techniques
OTO - NOTNLM
OT  - Ex vivo
OT  - Lung tissue functions
OT  - Precision-cut lung slices (PCLS)
EDAT- 2019/02/23 06:00
MHDA- 2019/07/12 06:00
CRDT- 2019/02/22 06:00
PHST- 2019/02/22 06:00 [entrez]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/07/12 06:00 [medline]
AID - 10.1007/978-1-4939-9086-3_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1940:297-311. doi: 10.1007/978-1-4939-9086-3_21.