PMID- 30788920
OWN - NLM
STAT- MEDLINE
DCOM- 20190509
LR  - 20190509
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 34
IP  - 5
DP  - 2018 May 8
TI  - [Effects of astragaloside-IV on the expression of inflammatory factor and 
      proliferation of glomerular mesangial cells induced by angiotensin Ⅱ].
PG  - 414-417 421
LID - 10.12047/j.cjap.5660.2018.094 [doi]
AB  - OBJECTIVE: To study the effects of astragaloside-IV (As-IV) on the expression of 
      inflammatory factor and proliferation of glomerular mesangial cells (GMCs) 
      induced by angiotensin Ⅱ(AngⅡ). METHODS: The in vitro model of diabetic 
      nephropathy(DN) was mimic by angiotensin Ⅱ (10(-6)mol/L)inducing GMCs injury. 
      Then the GMCs were treated with As-IV at different concentrations(25,50,100 
      mumol/L)for 48 hours. The proliferation of GMCs was detected by MTT. The level of 
      reactive oxidative species (ROS) was measured by flow cytometry. The expression 
      of monocyte chemoattractant protein-1(MCP-1) protein in supernatant was detected 
      by enzyme- linked immunosorbent assay (ELISA). The expression of transforming 
      growth factor-beta1(TGF-beta1) in GMCs was measured by Western blot. RESULTS: Compared 
      with model group, the proliferation of GMCs was inhibited in As-IV group. As-IV 
      decreased the level of intercellular ROS, down-regulated the secretion of MCP-1 
      and the expression of TGF-beta1 proteins. CONCLUSIONS: As-IV could inhibit cell 
      proliferation and inflammatory factors expression on GMCs induced by AngⅡ.
FAU - Xiong, Li
AU  - Xiong L
AD  - Department of Diagnostic, Wannan Medical College, Wuhu 241001, China.
FAU - Lyu, Meng-Juan
AU  - Lyu MJ
AD  - Department of Central Lab, Wannan Medical College, Wuhu 241001, China.
FAU - Dou, De-Yu
AU  - Dou DY
AD  - Department of Central Lab, Wannan Medical College, Wuhu 241001, China.
FAU - Ma, Yu-Hong
AU  - Ma YH
AD  - Department of Diagnostic, Wannan Medical College, Wuhu 241001, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Transforming Growth Factor beta1)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II
MH  - Blotting, Western
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - *Diabetic Nephropathies
MH  - Humans
MH  - *Mesangial Cells
MH  - Transforming Growth Factor beta1
OTO - NOTNLM
OT  - astragaloside-IV
OT  - monocyte chemoattractant protein-1
OT  - rat glomerular mesangial cells
OT  - reactive oxygen species
EDAT- 2019/02/23 06:00
MHDA- 2019/05/10 06:00
CRDT- 2019/02/22 06:00
PHST- 2019/02/22 06:00 [entrez]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/05/10 06:00 [medline]
AID - 10.12047/j.cjap.5660.2018.094 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 May 8;34(5):414-417 421. doi: 
      10.12047/j.cjap.5660.2018.094.