PMID- 30788921
OWN - NLM
STAT- MEDLINE
DCOM- 20190509
LR  - 20190509
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 34
IP  - 5
DP  - 2018 May 8
TI  - [Effects of laser stimulation with different power on oxygen consumption rate of 
      C(2)C(12) myoblasts in mice].
PG  - 418-421
LID - 10.12047/j.cjap.5667.2018.095 [doi]
AB  - OBJECTIVE: To investigate the effects of 650 nm laser irradiation on cell oxygen 
      consumption rate in C(2)C(12) myoblasts following different doses. METHODS: 
      C(2)C(12) cells were irradiated with 650 nm laser(lambda=650 nm, p=5 mW) with energy 
      densities of 0, 0.4, and 0.8 J/cm(2). Cell oxidative function was measured by 
      oxygen consumption rate kit. Protein expression of myogenic determination factor 
      (MyoD), peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha), 
      mammalian target of rapamycin (mTOR) and its phosphorylation were detected by 
      Western blot. RESULTS: Compared to the control group, the expression levels of 
      MyoD, PGC-1alpha protein were increased and cell oxygen consumption rate was promoted 
      in the low dose group(P<0.05). MyoD and PGC-1alpha protein expressions were also 
      increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased 
      significantly in the high dose group(P<0.05). CONCLUSIONS: 650 nm laser 
      irradiation that dose is 0.4 J/cm(2) enhances cell oxidative function, it related 
      to that proper dose laser irradiation promoted the expression of PGC-1a protein.
FAU - Feng, Yu
AU  - Feng Y
AD  - Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China.
FAU - Cheng, Ze-Peng
AU  - Cheng ZP
AD  - Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China.
FAU - Sun, Peng
AU  - Sun P
AD  - Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China.
FAU - Shi, Reng-Fei
AU  - Shi RF
AD  - Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Mice
MH  - Myoblasts
MH  - Oxidation-Reduction
MH  - *Oxygen Consumption
MH  - Transcription Factors
OTO - NOTNLM
OT  - C2C12 cells
OT  - PGC-1alpha
OT  - laser irradiation
OT  - oxygen consumption rate
EDAT- 2019/02/23 06:00
MHDA- 2019/05/10 06:00
CRDT- 2019/02/22 06:00
PHST- 2019/02/22 06:00 [entrez]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/05/10 06:00 [medline]
AID - 10.12047/j.cjap.5667.2018.095 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 May 8;34(5):418-421. doi: 
      10.12047/j.cjap.5667.2018.095.