PMID- 30790389
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20191230
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
VI  - 25
IP  - 4
DP  - 2019 May
TI  - Effects of miRNA-342-3p in modulating Hedgehog signaling pathway of human 
      umbilical cord mesenchymal stem cells by down-regulating Sufu.
PG  - 1147-1157
LID - 10.1111/odi.13068 [doi]
AB  - OBJECTIVE: Previously, we have shown that miRNA-342-3p was increased during 
      osteogenic differentiation of human umbilical mesenchymal stem cells (hUCMSCs) 
      via regulating the sonic hedgehog (Shh) pathway. In this study, our objective is 
      to further investigate the role of miRNA-342-3p in activation of Shh pathway by 
      targeting suppressor of fused protein (Sufu), a suppressor of transcriptional 
      factor Gli, as well as the potential interaction with transforming growth factor 
      beta (TGF-beta) signaling pathway during osteogenic induction of hUCMSCs. MATERIALS 
      AND METHODS: HUCMSCs that stable overexpression or knockdown of miRNA-342-3p were 
      established by infection with lentiviral vectors. mRNA and protein levels of 
      Hedgehog signaling pathway and osteogenic genes were measured by RT-qPCR and 
      western blot assays. Luciferase reporter assay was performed to test the direct 
      binding site of Sufu 5'UTR targeted by miRNA-342-3p. RESULTS: Overexpression of 
      miRNA-342-3p in hUCMSCs enhanced the expression of osteogenic genes by targeting 
      Sufu. And the potential of osteogenic differentiation of hUCMSCs was inhibited 
      while knocking down miRNA-342-3p. Meanwhile, induced the TGF-beta expression level 
      was also observed upon overexpressing miRNA-342-3p, suggesting activation of 
      TGF-beta signaling pathway was a potential mechanism of miRNA-342-3p-mediated 
      osteogenesis in hUCMSCs. CONCLUSIONS: Our findings provide new mechanistic 
      evidence that miRNA-342-3p might be a valuable therapeutic target in bone 
      regeneration.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights 
      reserved.
FAU - Qing, Ying
AU  - Qing Y
AUID- ORCID: 0000-0002-9551-8907
AD  - Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Huang, Mengqi
AU  - Huang M
AD  - Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Cao, Yingguang
AU  - Cao Y
AD  - Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Du, Tianfeng
AU  - Du T
AD  - Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Song, Ke
AU  - Song K
AD  - Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
LA  - eng
GR  - 81400490/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190311
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (Hedgehog Proteins)
RN  - 0 (MIRN342 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Repressor Proteins)
RN  - 0 (SUFU protein, human)
MH  - Bone Regeneration
MH  - Cell Differentiation
MH  - *Hedgehog Proteins
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs
MH  - *Osteogenesis
MH  - *Repressor Proteins
MH  - Signal Transduction
MH  - *Umbilical Cord
OTO - NOTNLM
OT  - Hedgehog signaling pathway
OT  - Sufu
OT  - hUCMSCs
OT  - miRNA-342-3p
OT  - osteogenesis
EDAT- 2019/02/23 06:00
MHDA- 2019/12/31 06:00
CRDT- 2019/02/22 06:00
PHST- 2018/10/19 00:00 [received]
PHST- 2019/02/03 00:00 [revised]
PHST- 2019/02/15 00:00 [accepted]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2019/02/22 06:00 [entrez]
AID - 10.1111/odi.13068 [doi]
PST - ppublish
SO  - Oral Dis. 2019 May;25(4):1147-1157. doi: 10.1111/odi.13068. Epub 2019 Mar 11.