PMID- 30794352
OWN - NLM
STAT- MEDLINE
DCOM- 20191121
LR  - 20191210
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 47
IP  - 7
DP  - 2019 Jul
TI  - Utility of Sry-Related HMG-Box Gene 10 (SOX10) as a marker of melanoma in 
      effusion cytology.
PG  - 653-658
LID - 10.1002/dc.24162 [doi]
AB  - BACKGROUND: The cytodiagnosis of melanoma in effusions can be challenging. 
      Although immunohistochemical (IHC) stains such as HMB45, Melan A, and S-100 are 
      often utilized; the role of Sry-related HMG-box gene 10 (SOX10) in the diagnosis 
      of melanoma in effusions has not been previously reported. METHODS: A total of 14 
      confirmed melanoma cases diagnosed on effusion cytology (nine pleural and five 
      peritoneal) were collected from 2000 to 2016. IHC stain for SOX10 was performed 
      and compared with HMB45, Melan A, and S-100. To evaluate the specificity of 
      SOX10, we stained 47 previously diagnosed nonmelanocytic malignant effusions. A 
      cut-off of >1 positively staining cells was considered positive. The intensity of 
      staining was graded as weak, moderate, and strong. RESULTS: All 14 melanoma cases 
      were positive for SOX10 and HMB45 (100%), compared to 12 cases (86%) using Melan 
      A and S-100. Among 47 previously diagnosed nonmelanocytic malignant effusions (4 
      malignant mesotheliomas, 12 mullerian tumors, 9 breast carcinomas, 9 lung 
      adenocarcinomas, and 13 hematologic tumors), SOX10 and HMB45 showed a specificity 
      of 98%, whereas Melan A and S100 had a specificity of 100%. CONCLUSIONS: The 
      sensitivity of SOX10 for melanoma in effusions is comparable with HMB45 with a 
      100% sensitivity. In terms of staining intensity, HMB45 appeared to be superior 
      as it showed 100% moderate to strong intensity compared to 72% for SOX10. All 
      four markers showed near-100% specificity in differentiating melanoma from 
      nonmelanocytic malignant effusions. A combination of HMB45 and SOX10 might be 
      useful as the initial stains of choice in diagnosing melanoma in effusion 
      cytology.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Dermawan, Josephine Kam Tai
AU  - Dermawan JKT
AUID- ORCID: 0000-0002-1139-2914
AD  - Department of Pathology, Robert J. Tomsich Pathology and Laboratory Medicine 
      Institute, Cleveland Clinic, Cleveland, Ohio.
FAU - Underwood, Dawn
AU  - Underwood D
AD  - Department of Pathology, Robert J. Tomsich Pathology and Laboratory Medicine 
      Institute, Cleveland Clinic, Cleveland, Ohio.
FAU - Policarpio-Nicolas, Maria Luisa
AU  - Policarpio-Nicolas ML
AUID- ORCID: 0000-0002-0375-1964
AD  - Department of Pathology, Robert J. Tomsich Pathology and Laboratory Medicine 
      Institute, Cleveland Clinic, Cleveland, Ohio.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20190222
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (SOX10 protein, human)
RN  - 0 (SOXE Transcription Factors)
SB  - IM
MH  - Ascitic Fluid/metabolism/*pathology
MH  - Biomarkers, Tumor/genetics/*metabolism/standards
MH  - Humans
MH  - Melanoma/metabolism/*pathology
MH  - Neoplasm Metastasis
MH  - Pleural Effusion, Malignant/metabolism/*pathology
MH  - SOXE Transcription Factors/genetics/*metabolism/standards
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - SOX10
OT  - cytology
OT  - immunohistochemistry
OT  - malignant effusions
OT  - melanoma
EDAT- 2019/02/23 06:00
MHDA- 2019/11/22 06:00
CRDT- 2019/02/23 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2019/02/03 00:00 [revised]
PHST- 2019/02/08 00:00 [accepted]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/11/22 06:00 [medline]
PHST- 2019/02/23 06:00 [entrez]
AID - 10.1002/dc.24162 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2019 Jul;47(7):653-658. doi: 10.1002/dc.24162. Epub 2019 Feb 
      22.