PMID- 30794657
OWN - NLM
STAT- MEDLINE
DCOM- 20191121
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 2
DP  - 2019
TI  - Wnt traffic from endoplasmic reticulum to filopodia.
PG  - e0212711
LID - 10.1371/journal.pone.0212711 [doi]
LID - e0212711
AB  - Wnts are a family of secreted palmitoleated glycoproteins that play key roles in 
      cell to cell communication during development and regulate stem cell compartments 
      in adults. Wnt receptors, downstream signaling cascades and target pathways have 
      been extensively studied while less is known about how Wnts are secreted and move 
      from producing cells to receiving cells. We used the synchronization system 
      called Retention Using Selective Hook (RUSH) to study Wnt trafficking from 
      endoplasmic reticulum to Golgi and then to plasma membrane and filopodia in real 
      time. Inhibition of porcupine (PORCN) or knockout of Wntless (WLS) blocked Wnt 
      exit from the ER. Wnt-containing vesicles paused at sub-cortical regions of the 
      plasma membrane before exiting the cell. Wnt-containing vesicles were associated 
      with filopodia extending to adjacent cells. These data visualize and confirm the 
      role of WLS and PORCN in ER exit of Wnts and support the role of filopodia in Wnt 
      signaling.
FAU - Moti, Naushad
AU  - Moti N
AD  - Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore.
FAU - Yu, Jia
AU  - Yu J
AUID- ORCID: 0000-0002-7004-9104
AD  - Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore.
FAU - Boncompain, Gaelle
AU  - Boncompain G
AD  - Institut Curie, PSL Research University, Sorbonne Universite, CNRS UMR144 "Cell 
      Biology and Cancer", Paris, France.
FAU - Perez, Franck
AU  - Perez F
AD  - Institut Curie, PSL Research University, Sorbonne Universite, CNRS UMR144 "Cell 
      Biology and Cancer", Paris, France.
FAU - Virshup, David M
AU  - Virshup DM
AUID- ORCID: 0000-0001-6976-850X
AD  - Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore.
AD  - Department of Pediatrics, Duke University, Durham, North Carolina, United States 
      of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190222
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Wnt Proteins)
SB  - IM
MH  - Cell Membrane/genetics/*metabolism
MH  - Endoplasmic Reticulum/genetics/*metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Protein Transport/genetics
MH  - Pseudopodia/genetics/*metabolism
MH  - Secretory Vesicles/genetics/*metabolism
MH  - Wnt Proteins/genetics/*metabolism
PMC - PMC6386245
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/02/23 06:00
MHDA- 2019/11/22 06:00
PMCR- 2019/02/22
CRDT- 2019/02/23 06:00
PHST- 2018/11/26 00:00 [received]
PHST- 2019/02/07 00:00 [accepted]
PHST- 2019/02/23 06:00 [entrez]
PHST- 2019/02/23 06:00 [pubmed]
PHST- 2019/11/22 06:00 [medline]
PHST- 2019/02/22 00:00 [pmc-release]
AID - PONE-D-18-33812 [pii]
AID - 10.1371/journal.pone.0212711 [doi]
PST - epublish
SO  - PLoS One. 2019 Feb 22;14(2):e0212711. doi: 10.1371/journal.pone.0212711. 
      eCollection 2019.