PMID- 30795311
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 88
IP  - 11
DP  - 2004 Nov
TI  - Detection and Characterization of a New Strain of Citrus Canker Bacteria from 
      Key/Mexican Lime and Alemow in South Florida.
PG  - 1179-1188
LID - 10.1094/PDIS.2004.88.11.1179 [doi]
AB  - In the Wellington and Lake Worth areas of Palm Beach County, FL, citrus canker 
      appeared on Key/Mexican lime (Citrus aurantiifolia) and alemow (C. macrophylla) 
      trees over a period of about 6 to 7 years before detection, but nearby 
      canker-susceptible citrus, such as grapefruit (C. x paradisi) and sweet orange 
      (C. sinensis), were unaffected. Colonies of the causal bacterium, isolated from 
      leaf, stem, and fruit lesions, appeared similar to the Asiatic group of strains 
      of Xanthomonas axonopodis pv. citri (Xac-A) on the nutrient agar plate, but the 
      growth on lima bean agar slants was less mucoid. The bacterium produced erumpent, 
      pustule-like lesions of typical Asiatic citrus canker syndrome after inoculation 
      into Key/Mexican lime, but brownish, flat, and necrotic lesions on the leaves of 
      Duncan grapefruit, Madame Vinous sweet orange, sour orange (C. aurantium), citron 
      (C. medica), Orlando tangelo (C. reticulata x C. x paradisi), and trifoliate 
      orange (Poncirus trifoliata). The bacterium did not react with the Xac-A specific 
      monoclonal antibody A1 using enzyme-linked immunosorbent assay (ELISA) and could 
      not be detected by polymerase chain reaction (PCR)-based assays using primers 
      selected for Xac-A. DNA reassociation analysis confirmed that the pathogen, 
      designated as Xac-A(W), was more closely related to Xac-A and Xac-A* strains than 
      X. axonopodis pv. aurantifolii or the citrus bacterial spot pathogen (X. 
      axonopodis pv. citrumelo). The strain can be easily differentiated from Xac-A and 
      Xac-A* using ELISA, PCR-based tests, fatty acid analysis, pulsed-field gel 
      electrophoresis of genomic DNA, and host specificity.
FAU - Sun, Xiaoan
AU  - Sun X
AD  - Florida Department of Agriculture & Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
FAU - Stall, Robert E
AU  - Stall RE
AD  - Florida Department of Agriculture & Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
FAU - Jones, Jeffrey B
AU  - Jones JB
AD  - University of Florida, Department of Plant Pathology, Gainesville 32611.
FAU - Cubero, Jaime
AU  - Cubero J
AD  - University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred 
      33850.
FAU - Gottwald, Tim R
AU  - Gottwald TR
AD  - USDA, Agricultural Research Service, U.S. Horticultural Research Laboratory, Ft. 
      Pierce, FL 34945.
FAU - Graham, James H
AU  - Graham JH
AD  - University of Florida, IFAS, Citrus Research and Education Center, Lake Alfred 
      33850.
FAU - Dixon, Wayne N
AU  - Dixon WN
AD  - Florida Department of Agriculture & Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
FAU - Schubert, Tim S
AU  - Schubert TS
AD  - Florida Department of Agriculture & Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
FAU - Chaloux, Paul H
AU  - Chaloux PH
AD  - USDA Citrus Canker Eradication Program, Plantation, FL 33313.
FAU - Stromberg, Verlyn K
AU  - Stromberg VK
AD  - Virginia Polytechnic Institute and State University, Department of Plant 
      Pathology, Physiology, and Weed Science, Blacksburg 24061.
FAU - Lacy, George H
AU  - Lacy GH
AD  - Virginia Polytechnic Institute and State University, Department of Plant 
      Pathology, Physiology, and Weed Science, Blacksburg 24061.
FAU - Sutton, Bruce D
AU  - Sutton BD
AD  - Florida Department of Agriculture & Consumer Services, Division of Plant 
      Industry, Gainesville 32614.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
OTO - NOTNLM
OT  - canker eradication
OT  - disease diagnosis
OT  - taxonomy of xanthomonads
EDAT- 2004/11/01 00:00
MHDA- 2004/11/01 00:01
CRDT- 2019/02/24 06:00
PHST- 2019/02/24 06:00 [entrez]
PHST- 2004/11/01 00:00 [pubmed]
PHST- 2004/11/01 00:01 [medline]
AID - 10.1094/PDIS.2004.88.11.1179 [doi]
PST - ppublish
SO  - Plant Dis. 2004 Nov;88(11):1179-1188. doi: 10.1094/PDIS.2004.88.11.1179.