PMID- 30796482
OWN - NLM
STAT- MEDLINE
DCOM- 20190718
LR  - 20190718
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 18
DP  - 2019 Jul
TI  - Graphene quantum dots enhanced ToF-SIMS for single-cell imaging.
PG  - 4025-4030
LID - 10.1007/s00216-019-01686-5 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has shown promising 
      applications in single-cell analysis owing to its high spatial resolution 
      molecular imaging capability. One of the main drawbacks hindering progress in 
      this field is the relatively low ionization efficiency for biological systems. 
      The complex chemical micro-environment in single cells typically causes severe 
      matrix effects, leading to significant signal suppression of biomolecules. In 
      this work, we investigated the signal enhancement effect of graphene quantum dots 
      (GE QDs) in ToF-SIMS analysis. A x 160 magnification of ToF-SIMS signal for 
      amiodarone casted on glass slide was observed by adding amino-functionalized GE 
      QDs (amino-GE QDs), which was significantly higher than adding previously 
      reported signal enhancement materials and hydroxyl group-functionalized GE QDs 
      (hydroxyl-GE QDs). A possible mechanism for GE QD-induced signal enhancement was 
      proposed. Further, effects of amino-GE QDs and hydroxyl-GE QDs on 
      amiodarone-treated breast cancer cells were compared. A significant signal 
      improvement for lipids and amiodarone was achieved using both types of GE QDs, 
      especially for amino-GE QDs. In addition, ToF-SIMS chemical mapping of single 
      cells with better quality was obtained after signal enhancement. Our strategy for 
      effective ToF-SIMS signal enhancement holds great potential for further 
      investigation of drug metabolism pathways and the interactions between the cell 
      and micro-environment.
FAU - Li, Hao-Wen
AU  - Li HW
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology, Shanghai, 200237, 
      China.
FAU - Hua, Xin
AU  - Hua X
AUID- ORCID: 0000-0003-1064-083X
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology, Shanghai, 200237, 
      China. xinhua@ecust.edu.cn.
FAU - Long, Yi-Tao
AU  - Long YT
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology, Shanghai, 200237, 
      China.
LA  - eng
GR  - 17ZR1407700/Natural Science Foundation of Shanghai/
GR  - 21834001/National Natural Science Foundation of China/
GR  - 2017-01-07-00-02-E00023/Innovation Program of Shanghai Municipal Education 
      Commission/
GR  - 17YF1403000/Shanghai Sailing Program/
GR  - 222201718001/Fundamental Research Funds for the Central University/
PT  - Journal Article
DEP - 20190222
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Breast Neoplasms/pathology
MH  - Female
MH  - Graphite/*chemistry
MH  - Humans
MH  - Quantum Dots/*chemistry
MH  - *Single-Cell Analysis
MH  - Spectrometry, Mass, Secondary Ion/*methods
OTO - NOTNLM
OT  - Graphene quantum dots
OT  - Signal enhancement
OT  - Single-cell analysis
OT  - Time-of-flight secondary ion mass spectrometry
EDAT- 2019/02/24 06:00
MHDA- 2019/07/19 06:00
CRDT- 2019/02/24 06:00
PHST- 2018/09/30 00:00 [received]
PHST- 2019/02/12 00:00 [accepted]
PHST- 2019/01/31 00:00 [revised]
PHST- 2019/02/24 06:00 [pubmed]
PHST- 2019/07/19 06:00 [medline]
PHST- 2019/02/24 06:00 [entrez]
AID - 10.1007/s00216-019-01686-5 [pii]
AID - 10.1007/s00216-019-01686-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Jul;411(18):4025-4030. doi: 10.1007/s00216-019-01686-5. 
      Epub 2019 Feb 22.