PMID- 30796669
OWN - NLM
STAT- MEDLINE
DCOM- 20190806
LR  - 20200225
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 26
IP  - 11
DP  - 2019 Apr
TI  - Intergenerational reproductive toxicity of chlordecone in male Caenorhabditis 
      elegans.
PG  - 11279-11287
LID - 10.1007/s11356-019-04519-1 [doi]
AB  - Chlordecone (CLD), also named Kepone, is a synthetic organochlorine pesticide. As 
      one of the common persistent organic pollutants (POPs) in nature, CLD has a 
      profound impact on the environment and human health. The study aims to 
      investigate the reproductive toxicity effects of CLD on male Caenorhabditis 
      elegans and on progeny. L1-stage male nematodes were exposed to the control group 
      (M9 solution) and four dose groups (0.02, 0.2, 2, and 20 mug/L). After exposure 
      for 48 h, the male nematodes were picked to mating experiment and progeny 
      experiment that the number of progeny and the time of observation in male parent 
      and in F1 generation were counted; the number of germ cells and the number of 
      sperm in the meiotic division of male nematodes were counted by staining with 
      dimercaptophenyl hydrazine (DAPI), and the nematode gland area was observed under 
      the bright field of the microscope. In male nematodes, the results showed that a 
      number of progeny were 351.20 +/- 31.40, 321.60 +/- 24.70, 307.30 +/- 19.30, 
      240.10 +/- 27.60, and 227.90 +/- 22.70 (P < 0.05); the generation times were 
      55.80 +/- 1.95 h, 56.40 +/- 1.60 h, 56.70 +/- 0.92 h, 60.80 +/- 0.95 h, and 
      69.60 +/- 1.97 h (P < 0.05); relative areas of gonad were (99.80 +/- 6.27)%, 
      (93.00 +/- 1.70)%, (85.00 +/- 1.70)%, (70.70 +/- 9.81)%, and (60.00 +/- 5.23)% 
      (P < 0.05); DAPI staining results showed the number of germ cells in meiosis area 
      were 191.00 +/- 10.97, 181.10 +/- 15.56, 177.00 +/- 9.20, 147.50 +/- 10.56, and 
      139.30 +/- 23.79 (P < 0.05); the sperm numbers were 335.60 +/- 21.31, 308.60 +/- 19.60, 
      306.00 +/- 11.23, 260.10 +/- 27.41, and 255.00 +/- 3.72 (P < 0.05). In the F1 
      generation, the progeny numbers were 328.10 +/- 22.28, 167.50 +/- 15.30, 
      150.00 +/- 13.65, 131.30 +/- 18.40, and 130.20 +/- 16.17 (P < 0.05); the generation 
      times were 55.50 +/- 2.36, 71.10 +/- 0.97, 70.90 +/- 0.52, 74.10 +/- 2.07, and 
      73.90 +/- 1.35 h (P < 0.05). The groups are grouped in order as M9 solution, 0.02, 
      0.2, 2, and 20 mug/L. The results revealed that CLD caused decrease in progeny 
      number, relative area of gonad, number of germ cells, and sperm number and 
      prolonged the generation time in the male nematode. In offspring grown up without 
      CLD, the effect of CLD on generation time and sperm number can still be observed 
      on offspring. In conclusion, CLD induces male nematode reproductive toxicity and 
      causes defects in offspring.
FAU - Dai, Shuhao
AU  - Dai S
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China.
FAU - Miao, Yan
AU  - Miao Y
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China.
FAU - Liu, Ran
AU  - Liu R
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China.
FAU - Pu, Yuepu
AU  - Pu Y
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China.
FAU - Yin, Lihong
AU  - Yin L
AUID- ORCID: 0000-0002-0444-7980
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, Nanjing, 210009, China. 
      lhyin@seu.edu.cn.
LA  - eng
GR  - 81273123/National Natural Science Foundation of China/
GR  - 81872588/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190222
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Pesticides)
RN  - RG5XJ88UDF (Chlordecone)
MH  - Animals
MH  - Caenorhabditis elegans/*drug effects/*physiology
MH  - Chlordecone/*toxicity
MH  - Female
MH  - Male
MH  - Pesticides/*toxicity
MH  - Reproduction/drug effects
MH  - Sexual Behavior, Animal/drug effects
MH  - Sperm Count
OTO - NOTNLM
OT  - Caenorhabditis elegans
OT  - Chlordecone
OT  - Intergenerational
OT  - Offspring
OT  - Reproductive toxicity
OT  - Sperm
EDAT- 2019/02/24 06:00
MHDA- 2019/08/07 06:00
CRDT- 2019/02/24 06:00
PHST- 2018/09/26 00:00 [received]
PHST- 2019/02/06 00:00 [accepted]
PHST- 2019/02/24 06:00 [pubmed]
PHST- 2019/08/07 06:00 [medline]
PHST- 2019/02/24 06:00 [entrez]
AID - 10.1007/s11356-019-04519-1 [pii]
AID - 10.1007/s11356-019-04519-1 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2019 Apr;26(11):11279-11287. doi: 
      10.1007/s11356-019-04519-1. Epub 2019 Feb 22.