PMID- 30796780
OWN - NLM
STAT- MEDLINE
DCOM- 20191008
LR  - 20191210
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 57
IP  - 5
DP  - 2019 May 1
TI  - Development of a Rapid Method for the Determination of Phenolic Antioxidants in 
      Dark Chocolate Using Ultra Performance Liquid Chromatography Coupled to Orbitrap 
      Mass Spectrometry.
PG  - 434-442
LID - 10.1093/chromsci/bmz013 [doi]
AB  - Development of a new comprehensive method based on one-step liquid-liquid 
      extraction (LLE) combined with ultra-high-performance liquid 
      chromatography-Orbitrap high resolution mass spectrometry (UHPLC-Orbitrap-HRMS) 
      for the determination of eight phenolic compounds in dark chocolate is 
      demonstrated. The study focuses on the optimization of sample preparation, 
      chromatographic separation and Orbitrap-HRMS detection parameters to adjust 
      selectivity and sensitivity of the method. The LLE mixture of formic 
      acid-water-acetonitrile (2:58:40, v/v) and hexane at the component ratio of 1:1 
      (v/v) was applied for sample preparation. The inter-day and intra-day precision 
      expressed from the relative standard deviations (RSDs) ranged between 5.50-14.2% 
      and 5.60-16.2%. The quantification limits of the target analytes were between 0.1 
      and 1.0 mg/kg. The method was applied for the analysis of twenty-six samples of 
      dark chocolate with various cocoa solid contents (30-77%) collected from the 
      markets in Latvia. The total levels of the phenolic contents ranged between 330 
      and 2631 mg/kg. The study demonstrates advantages of the Orbitrap-HRMS method for 
      the study of phenolic compounds in dark chocolate.
CI  - (c) The Author(s) 2019. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Rusko, Janis
AU  - Rusko J
AD  - Laboratory of Food and Environmental Investigations, Institute of Food Safety, 
      Animal Health and Environment "BIOR", Lejupes iela 3, Riga, Latvia.
FAU - Pugajeva, Iveta
AU  - Pugajeva I
AD  - Laboratory of Food and Environmental Investigations, Institute of Food Safety, 
      Animal Health and Environment "BIOR", Lejupes iela 3, Riga, Latvia.
AD  - Department of Chemistry, The Academic Center for Natural Sciences of the 
      University of Latvia, Jelgavas iela 1, Riga, Latvia.
FAU - Perkons, Ingus
AU  - Perkons I
AD  - Laboratory of Food and Environmental Investigations, Institute of Food Safety, 
      Animal Health and Environment "BIOR", Lejupes iela 3, Riga, Latvia.
FAU - Reinholds, Ingars
AU  - Reinholds I
AD  - Laboratory of Food and Environmental Investigations, Institute of Food Safety, 
      Animal Health and Environment "BIOR", Lejupes iela 3, Riga, Latvia.
AD  - Department of Chemistry, The Academic Center for Natural Sciences of the 
      University of Latvia, Jelgavas iela 1, Riga, Latvia.
FAU - Bartkiene, Elena
AU  - Bartkiene E
AD  - Department of Food Safety and Quality, Lithuanian University of Health Sciences, 
      A. Mickeviciaus g. 9, Kaunas, Lithuania.
FAU - Bartkevics, Vadims
AU  - Bartkevics V
AD  - Laboratory of Food and Environmental Investigations, Institute of Food Safety, 
      Animal Health and Environment "BIOR", Lejupes iela 3, Riga, Latvia.
AD  - Department of Chemistry, The Academic Center for Natural Sciences of the 
      University of Latvia, Jelgavas iela 1, Riga, Latvia.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Antioxidants)
RN  - 0 (Phenols)
SB  - IM
MH  - Antioxidants/*analysis
MH  - Chocolate/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Mass Spectrometry/*methods
MH  - Phenols/*analysis
OTO - NOTNLM
OT  - Dark chocolate
OT  - liquid-liquid extraction
OT  - orbitrap high-resolution mass spectrometry
OT  - phenolic compounds
OT  - ultra-high-performance liquid chromatography
EDAT- 2019/02/24 06:00
MHDA- 2019/10/09 06:00
CRDT- 2019/02/24 06:00
PHST- 2017/12/12 00:00 [received]
PHST- 2019/01/08 00:00 [revised]
PHST- 2019/02/01 00:00 [accepted]
PHST- 2019/02/24 06:00 [pubmed]
PHST- 2019/10/09 06:00 [medline]
PHST- 2019/02/24 06:00 [entrez]
AID - 5363947 [pii]
AID - 10.1093/chromsci/bmz013 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2019 May 1;57(5):434-442. doi: 10.1093/chromsci/bmz013.