PMID- 30797459
OWN - NLM
STAT- MEDLINE
DCOM- 20190320
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1056
DP  - 2019 May 16
TI  - Development and validation of a fully automated solid phase microextraction high 
      throughput method for quantitative analysis of multiresidue veterinary drugs in 
      chicken tissue.
PG  - 34-46
LID - S0003-2670(18)31493-4 [pii]
LID - 10.1016/j.aca.2018.12.044 [doi]
AB  - This paper presents the development and validation of a fully automated, 
      high-throughput multiclass, multiresidue method for quantitative analysis of 77 
      veterinary drugs in chicken muscle via direct immersion solid phase 
      microextraction (DI-SPME) and ultra-high pressure liquid 
      chromatography-electrospray ionization - tandem mass spectrometry 
      (UHPLC-ESI-MS/MS). The selected drugs represent more than 12 different classes of 
      drugs characterized by varying physical and chemical properties. A 
      Hydrophilic-lipophilic balance (HLB)/polyacrylonitrile (PAN) extraction phase, 
      prepared using HLB particles synthesized in-house, yielded the best 
      extraction/desorption performance among four different SPME extraction phases 
      evaluated in the current work. The developed SPME method was optimized in terms 
      of SPME coating and geometry, desorption solvent, extraction and rinsing 
      conditions, and extraction and desorption times. Multivariate analysis was 
      performed to determine the optimal desorption solvent for the proposed 
      application. The developed method was validated according to the Food and Drug 
      Administration (FDA) guidelines, taking into account Canadian maximum residue 
      limits (MRLs) and US maximum tolerance levels for veterinary drugs in meat. 
      Method accuracy ranged from 80 to 120% for at least 73 compounds, with relative 
      standard deviation of 1-15%. Inter-day precision ranged from 4 to 15% for 70 
      compounds. Determination coefficients values were higher than 0.991 for all 
      compounds under study with no significant lack of fit (p > 0.05) at the 5% level. 
      In terms of limits of quantitation, the method was able to meet both Canadian and 
      US regulatory levels for all compounds under study.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Khaled, Abir
AU  - Khaled A
AD  - Department of Chemistry, University of Waterloo, 200 University Avenue West, 
      Waterloo, Ontario, N2L 3G1, Canada.
FAU - Gionfriddo, Emanuela
AU  - Gionfriddo E
AD  - Department of Chemistry, University of Waterloo, 200 University Avenue West, 
      Waterloo, Ontario, N2L 3G1, Canada; Department of Chemistry and Biochemistry, The 
      University of Toledo, Toledo, OH, USA.
FAU - Acquaro, Vinicius Jr
AU  - Acquaro V Jr
AD  - Department of Chemistry, University of Waterloo, 200 University Avenue West, 
      Waterloo, Ontario, N2L 3G1, Canada; Departamento de Quimica, Faculdade de 
      Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, SP, 
      Brazil.
FAU - Singh, Varoon
AU  - Singh V
AD  - Department of Chemistry, University of Waterloo, 200 University Avenue West, 
      Waterloo, Ontario, N2L 3G1, Canada.
FAU - Pawliszyn, Janusz
AU  - Pawliszyn J
AD  - Department of Chemistry, University of Waterloo, 200 University Avenue West, 
      Waterloo, Ontario, N2L 3G1, Canada. Electronic address: janusz@uwaterloo.ca.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20181228
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Solvents)
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Animals
MH  - Automation
MH  - *Chickens
MH  - Drug Residues/*analysis/chemistry/*isolation & purification
MH  - Food Contamination/*analysis
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Solid Phase Microextraction/*methods
MH  - Solvents/chemistry
MH  - Time Factors
MH  - Veterinary Drugs/*analysis/chemistry/*isolation & purification
OTO - NOTNLM
OT  - Method development and validation
OT  - Multi-class multi-residue analysis
OT  - Solid phase microextraction
OT  - UHPLC-MS/MS
OT  - Veterinary drugs
EDAT- 2019/02/25 06:00
MHDA- 2019/03/21 06:00
CRDT- 2019/02/25 06:00
PHST- 2018/10/30 00:00 [received]
PHST- 2018/12/20 00:00 [revised]
PHST- 2018/12/21 00:00 [accepted]
PHST- 2019/02/25 06:00 [entrez]
PHST- 2019/02/25 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
AID - S0003-2670(18)31493-4 [pii]
AID - 10.1016/j.aca.2018.12.044 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2019 May 16;1056:34-46. doi: 10.1016/j.aca.2018.12.044. Epub 2018 
      Dec 28.