PMID- 30800583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 9
IP  - 3
DP  - 2019 Mar
TI  - Comparative de novo flower transcriptome analysis of polygamodioecious tree 
      Garcinia indica.
PG  - 72
LID - 10.1007/s13205-019-1601-3 [doi]
LID - 72
AB  - To extend our understanding of molecular mechanism of sex determination in 
      agro-economically important, polygamodioecious tree Garcinia indica (Kokum), 
      high-throughput, next-generation flower transcriptome sequencing (NGS), and 
      comparative analyses were performed to investigate differentially expressed gene 
      in bisexual, female, and male flowers. A total of 49414 unigenes in BS, 45944 
      unigenes in FL, and 49028 unigenes in ML flowers were annotated. KO annotations 
      revealed that 25 functional categories were large number of genes which were 
      annotated to 'signal transduction'. We identified 33 genes for 'auxin response 
      factor' and 50 for 'ethylene-responsive factor' whose expression changed 
      significantly in all the three paired library combinations. Furthermore, key 
      regulators of floral development such as FLC, SVP, AP1, AP2, AP3, AG, AGL2, AGL4, 
      AGL9, and PI were identified. A total of 327 differentially expressed MADS-box 
      genes were identified in G. indica transcriptome. Analysis of MADS-box genes 
      identified five genes such as MADS AGL11, CRS2-associated factor chloroplastic, 
      conserved hypothetical protein, uncharacterized protein LOC104422218, and 
      MADS-box JOINTLESS-like isoform X3 significantly expressed in only FL flower. In 
      addition, number of DEGs like dynamin 2A, auxin response factor, and spermidine 
      synthase involved in sex expression and reproduction were discovered. The 
      expression patterns of selected genes matched well with the expression levels of 
      unigenes by transcriptome sequencing. Our large-scale comparative analyses may 
      provide valuable hints for the next insights into the molecular mechanism of sex 
      determination in G. indica.
FAU - Patil, Reshma V
AU  - Patil RV
AD  - School of Nanoscience and Biotechnology, Shivaji University, Kolhapur, 
      Maharashtra India. ISNI: 0000 0001 0709 7763. GRID: grid.412574.1
FAU - Pawar, Kiran D
AU  - Pawar KD
AD  - School of Nanoscience and Biotechnology, Shivaji University, Kolhapur, 
      Maharashtra India. ISNI: 0000 0001 0709 7763. GRID: grid.412574.1
LA  - eng
PT  - Journal Article
DEP - 20190209
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC6368908
OTO - NOTNLM
OT  - Floral development
OT  - Garcinia indica
OT  - MADS-box gene
OT  - Polygamodioecious
OT  - Sex determination
OT  - Transcriptome
COIS- Compliance with ethical standardsThere is no conflict of interest.
EDAT- 2019/02/26 06:00
MHDA- 2019/02/26 06:01
PMCR- 2020/03/01
CRDT- 2019/02/26 06:00
PHST- 2018/09/28 00:00 [received]
PHST- 2019/01/31 00:00 [accepted]
PHST- 2019/02/26 06:00 [entrez]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2019/02/26 06:01 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - 1601 [pii]
AID - 10.1007/s13205-019-1601-3 [doi]
PST - ppublish
SO  - 3 Biotech. 2019 Mar;9(3):72. doi: 10.1007/s13205-019-1601-3. Epub 2019 Feb 9.