PMID- 30801904
OWN - NLM
STAT- MEDLINE
DCOM- 20200805
LR  - 20200805
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 33
IP  - 6
DP  - 2019 Jun
TI  - The effect of N-acetylcysteine supplementation on the oxidative stress levels, 
      apoptosis, DNA damage, and hematopoietic effect in pesticide-exposed fish blood.
PG  - e22311
LID - 10.1002/jbt.22311 [doi]
AB  - Cysteine is important for protein synthesis, detoxification, and diverse 
      metabolic functions. However, cysteine metabolism has been poorly described in 
      fish, and the role of the therapeutic effect in pesticide toxicology on aquatic 
      organisms is unknown. The aim of this study was to determine the effects of 
      regular cysteine treatment on the hematology, biochemistry, apoptosis, oxidative 
      DNA damage, and antioxidant parameters in fish blood after chemical application. 
      Therefore, fish were exposed to cypermethrin for 2 weeks. Then two different 
      concentrations of N-acetylcysteine (NAC) were applied for a 4-day treatment 
      period and compared with the group of the self-healing process. At the end of the 
      treatment, the hematological index, blood biochemical parameters, paraoxonase 
      (PON), arylesterase (ARE), and myeloperoxidase (MPO) activities in the fish blood 
      samples were investigated. With regard to the hematological parameters, 
      statistical differences were obtained except for mean corpuscular hemoglobin 
      (MCH) and mean corpuscular hemoglobin concentration (MCHC) (P < 0.05). Enzyme 
      activities (ARE, PON, and MPO), as well as some biochemical parameters (creatinin 
      [Cre], alanine amino transferase, total glyceride, alkaline phosphatase, iron, 
      calcium, low density lipoprotein-cholesterol [LDL-C], sodium, and potassium), 
      were found to be importantly different among all groups at the P < 0.05 level, 
      while 8-hydroxydeoxyguanosine and caspase-3 levels were determined to be high in 
      the pesticide group but decreased significantly in NAC-treated groups ( 
      P < 0.05). According to the results of the study, acute cysteine treatment showed 
      an ameliorative effect on the hematological index, biochemical parameters, PON, 
      MPO, and ARE in the blood in the all the treatment group fish. The positive 
      effect of NAC on protein synthesis, detoxification, and diverse metabolic 
      functions against cypermethrin toxicity was more effective in 1.0 mM NAC. NAC has 
      an important therapeutic effect on pesticide-induced hematoxicity for fish in 
      terms of all the data.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Ucar, Arzu
AU  - Ucar A
AD  - Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, 
      Turkey.
FAU - Ozgeris, Fatma Betul
AU  - Ozgeris FB
AD  - Department of Nutrition and Dietetics, Faculty of Health Sciences, Ataturk 
      University, Erzurum, Turkey.
FAU - Yeltekin, Asli Cilingir
AU  - Yeltekin AC
AD  - Department of Chemistry, Faculty of Science, University of Yuzuncu Yil, Van, 
      Turkey.
FAU - Parlak, Veysel
AU  - Parlak V
AD  - Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, 
      Turkey.
FAU - Alak, Gonca
AU  - Alak G
AUID- ORCID: 0000-0002-7539-1152
AD  - Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, 
      Turkey.
FAU - Keles, Mevlut Sait
AU  - Keles MS
AD  - Department of Medical Biochemistry, Faculty of Medical, Ataturk University, 
      Erzurum, Turkey.
FAU - Atamanalp, Muhammed
AU  - Atamanalp M
AD  - Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20190224
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Fish Proteins)
RN  - 0 (Pesticides)
RN  - 0 (Pyrethrins)
RN  - 1TR49121NP (cypermethrin)
RN  - EC 1.- (Oxidoreductases)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - *DNA Damage
MH  - Fish Proteins/metabolism
MH  - Hematopoiesis/*drug effects
MH  - Oncorhynchus mykiss/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - Oxidoreductases/metabolism
MH  - Pesticides/*toxicity
MH  - Pyrethrins/*toxicity
OTO - NOTNLM
OT  - 8-OHdG
OT  - MPO
OT  - NAC
OT  - caspase-3
OT  - fish
OT  - hematology
EDAT- 2019/02/26 06:00
MHDA- 2020/08/06 06:00
CRDT- 2019/02/26 06:00
PHST- 2018/11/15 00:00 [received]
PHST- 2019/01/10 00:00 [revised]
PHST- 2019/01/29 00:00 [accepted]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2020/08/06 06:00 [medline]
PHST- 2019/02/26 06:00 [entrez]
AID - 10.1002/jbt.22311 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2019 Jun;33(6):e22311. doi: 10.1002/jbt.22311. Epub 2019 
      Feb 24.