PMID- 30802499
OWN - NLM
STAT- MEDLINE
DCOM- 20200109
LR  - 20200109
IS  - 1095-953X (Electronic)
IS  - 0969-9961 (Linking)
VI  - 127
DP  - 2019 Jul
TI  - Manipulation of microbiota reveals altered callosal myelination and white matter 
      plasticity in a model of Huntington disease.
PG  - 65-75
LID - S0969-9961(18)30551-5 [pii]
LID - 10.1016/j.nbd.2019.02.011 [doi]
AB  - Structural and molecular myelination deficits represent early pathological 
      features of Huntington disease (HD). Recent evidence from germ-free (GF) animals 
      suggests a role for microbiota-gut-brain bidirectional communication in the 
      regulation of myelination. In this study, we aimed to investigate the impact of 
      microbiota on myelin plasticity and oligodendroglial population dynamics in the 
      mixed-sex BACHD mouse model of HD. Ultrastructural analysis of myelin in the 
      corpus callosum revealed alterations of myelin thickness in BACHD GF compared to 
      specific-pathogen free (SPF) mice, whereas no differences were observed between 
      wild-type (WT) groups. In contrast, myelin compaction was altered in all groups 
      when compared to WT SPF animals. Levels of myelin-related proteins were generally 
      reduced, and the number of mature oligodendrocytes was decreased in the 
      prefrontal cortex under GF compared to SPF conditions, regardless of genotype. 
      Minor differences in commensal bacteria at the family and genera levels were 
      found in the gut microbiota of BACHD and WT animals housed in standard living 
      conditions. Our findings indicate complex effects of a germ-free status on 
      myelin-related characteristics, and highlight the adaptive properties of 
      myelination as a result of environmental manipulation.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Radulescu, Carola I
AU  - Radulescu CI
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore; Department of Psychology, 
      The University of Sheffield, S1 2LT, UK.
FAU - Garcia-Miralles, Marta
AU  - Garcia-Miralles M
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore.
FAU - Sidik, Harwin
AU  - Sidik H
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore.
FAU - Bardile, Costanza Ferrari
AU  - Bardile CF
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore.
FAU - Yusof, Nur Amirah Binte Mohammad
AU  - Yusof NABM
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore.
FAU - Lee, Hae Ung
AU  - Lee HU
AD  - Lee Kong Chian School of Medicine, Nanyang Technological University, 637551, 
      Singapore.
FAU - Ho, Eliza Xin Pei
AU  - Ho EXP
AD  - GIS Efficient Rapid Microbial Sequencing, Genome Institute of Singapore, A*STAR, 
      138672, Singapore.
FAU - Chu, Collins Wenhan
AU  - Chu CW
AD  - GIS Efficient Rapid Microbial Sequencing, Genome Institute of Singapore, A*STAR, 
      138672, Singapore.
FAU - Layton, Emma
AU  - Layton E
AD  - GIS Efficient Rapid Microbial Sequencing, Genome Institute of Singapore, A*STAR, 
      138672, Singapore.
FAU - Low, Donovan
AU  - Low D
AD  - Singapore Immunology Network, A*STAR, 138648, Singapore.
FAU - De Sessions, Paola Florez
AU  - De Sessions PF
AD  - GIS Efficient Rapid Microbial Sequencing, Genome Institute of Singapore, A*STAR, 
      138672, Singapore.
FAU - Pettersson, Sven
AU  - Pettersson S
AD  - Lee Kong Chian School of Medicine, Nanyang Technological University, 637551, 
      Singapore; Singapore Centre for Environmental Life Sciences Engineering, 60 
      Nanyang Drive, 637551, Singapore.
FAU - Ginhoux, Florent
AU  - Ginhoux F
AD  - Singapore Immunology Network, A*STAR, 138648, Singapore.
FAU - Pouladi, Mahmoud A
AU  - Pouladi MA
AD  - Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, 
      Technology and Research (A*STAR), 138648, Singapore; Department of Medicine, 
      National University of Singapore, 117597, Singapore; Department of Physiology, 
      National University of Singapore, 117597, Singapore. Electronic address: 
      map@pouladilab.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190223
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
SB  - IM
RPI - Neurobiol Dis. 2020 Feb;135:104744. PMID: 31931139
MH  - Animals
MH  - Corpus Callosum/*pathology
MH  - Disease Models, Animal
MH  - Gastrointestinal Microbiome/*physiology
MH  - Huntington Disease/*microbiology/pathology
MH  - Mice
MH  - Myelin Sheath/*pathology
MH  - Neuronal Plasticity/*physiology
MH  - White Matter/*pathology
OTO - NOTNLM
OT  - BACHD
OT  - Germ-free
OT  - Huntington disease
OT  - Microbiome
OT  - Microbiota
OT  - Myelination
OT  - Oligodendrocytes
OT  - White matter
EDAT- 2019/02/26 06:00
MHDA- 2020/01/10 06:00
CRDT- 2019/02/26 06:00
PHST- 2018/09/04 00:00 [received]
PHST- 2019/02/02 00:00 [revised]
PHST- 2019/02/20 00:00 [accepted]
PHST- 2019/02/26 06:00 [pubmed]
PHST- 2020/01/10 06:00 [medline]
PHST- 2019/02/26 06:00 [entrez]
AID - S0969-9961(18)30551-5 [pii]
AID - 10.1016/j.nbd.2019.02.011 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2019 Jul;127:65-75. doi: 10.1016/j.nbd.2019.02.011. Epub 2019 Feb 
      23.